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Mechanism of tumor and liver concentration of /sup 67/Ga: /sup 67/Ga binding substances in tumor tissues and liver

Abstract

Tumor-bearing animals were administered with /sup 67/Ga citrate and tumor homogenates, from which nuclear fraction was removed, and mitochondrial fraction of the host livers were digested with protease (pronase P). After digestion, the supernatants of the reaction mixtures were applied to a Sephadex G-100 column. Resultant eluates were analyzed for radioactivity, protein, uronic acid and sialic acids. Three peaks of radioactivity were obtained by gel filtration. The first peak eluted in the void volume contained a species whose molecular weight exceeded 40,000. The second peak consisted of substances with molecular weights of 9400-40,000. Radioactivity in the third peak was from liberated gallium-67. /sup 67/Ga in the second peak was bound to acid mucopolysaccharide and/or the sulfated carbohydrate chain of sulfated glycoprotein. It was thought that /sup 67/Ga in the first peak might be bound to some acid mucopolysaccharides. Considering the results of cellulose acetate electrophoresis, /sup 67/Ga in the second peak seemed to be bound to acid mucopolysaccharide which contained no uronic acids, and/or to the sulfated carbohydrate chain of sulfated glycoprotein. It was concluded that /sup 67/Ga was bound to the acid mucopolysaccharides and/or the sulfated carbohydrate chain of sulfated glycoprotein in tumor tissues and liver lysosomes.
Publication Date:
Jan 01, 1983
Product Type:
Journal Article
Reference Number:
EDB-84-067140
Resource Relation:
Journal Name: Int. J. Nucl. Med. Biol.; (United Kingdom); Journal Volume: 10:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; GALLIUM 67; METABOLISM; LIVER; CHEMICAL ANALYSIS; NEOPLASMS; RADIOPHARMACEUTICALS; CITRATES; COMPARATIVE EVALUATIONS; GLUCOPROTEINS; LIQUID COLUMN CHROMATOGRAPHY; MOLECULAR WEIGHT; POLYSACCHARIDES; UPTAKE; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CARBOXYLIC ACID SALTS; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GALLIUM ISOTOPES; GLANDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; LABELLED COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; SACCHARIDES; SEPARATION PROCESSES; 560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987); 550501 - Metabolism- Tracer Techniques
OSTI ID:
5126005
Research Organizations:
Kanazawa Univ., Japan
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJNMC
Submitting Site:
HEDB
Size:
Pages: 1-9
Announcement Date:

Citation Formats

Ando, A, Ando, I, Hiraki, T, Takeshita, M, and Hisada, K. Mechanism of tumor and liver concentration of /sup 67/Ga: /sup 67/Ga binding substances in tumor tissues and liver. United Kingdom: N. p., 1983. Web. doi:10.1016/0047-0740(83)90026-8.
Ando, A, Ando, I, Hiraki, T, Takeshita, M, & Hisada, K. Mechanism of tumor and liver concentration of /sup 67/Ga: /sup 67/Ga binding substances in tumor tissues and liver. United Kingdom. doi:10.1016/0047-0740(83)90026-8.
Ando, A, Ando, I, Hiraki, T, Takeshita, M, and Hisada, K. 1983. "Mechanism of tumor and liver concentration of /sup 67/Ga: /sup 67/Ga binding substances in tumor tissues and liver." United Kingdom. doi:10.1016/0047-0740(83)90026-8. https://www.osti.gov/servlets/purl/10.1016/0047-0740(83)90026-8.
@misc{etde_5126005,
title = {Mechanism of tumor and liver concentration of /sup 67/Ga: /sup 67/Ga binding substances in tumor tissues and liver}
author = {Ando, A, Ando, I, Hiraki, T, Takeshita, M, and Hisada, K}
abstractNote = {Tumor-bearing animals were administered with /sup 67/Ga citrate and tumor homogenates, from which nuclear fraction was removed, and mitochondrial fraction of the host livers were digested with protease (pronase P). After digestion, the supernatants of the reaction mixtures were applied to a Sephadex G-100 column. Resultant eluates were analyzed for radioactivity, protein, uronic acid and sialic acids. Three peaks of radioactivity were obtained by gel filtration. The first peak eluted in the void volume contained a species whose molecular weight exceeded 40,000. The second peak consisted of substances with molecular weights of 9400-40,000. Radioactivity in the third peak was from liberated gallium-67. /sup 67/Ga in the second peak was bound to acid mucopolysaccharide and/or the sulfated carbohydrate chain of sulfated glycoprotein. It was thought that /sup 67/Ga in the first peak might be bound to some acid mucopolysaccharides. Considering the results of cellulose acetate electrophoresis, /sup 67/Ga in the second peak seemed to be bound to acid mucopolysaccharide which contained no uronic acids, and/or to the sulfated carbohydrate chain of sulfated glycoprotein. It was concluded that /sup 67/Ga was bound to the acid mucopolysaccharides and/or the sulfated carbohydrate chain of sulfated glycoprotein in tumor tissues and liver lysosomes.}
doi = {10.1016/0047-0740(83)90026-8}
journal = {Int. J. Nucl. Med. Biol.; (United Kingdom)}
volume = {10:1}
journal type = {AC}
place = {United Kingdom}
year = {1983}
month = {Jan}
}