You need JavaScript to view this

Somatic embryogenesis in cassava: A tool for mutation breeding

Abstract

Cassava is an important food and livestock feed crop. The effect of gamma radiation on somatic embryogenesis and plant regeneration in cassava clones of African origin was investigated. Explants from young leaves of cassava were cultured on MS medium, supplemented with 18.1 mM 2,4-D and 2 mM CuSO4, solidified with 0.3% Phytagel. Compact and friable calli were observed after 10-15 days of explant culture in dark, which produced somatic embryos in all but one clone. The somatic embryos showed morphological aberrations, such as fused cotyledons, lack of meristematic tip, epicotyl elongation, and had low germination rate; desiccation of embryos increased germination. Histological study showed that the somatic embryos were of multicellular origin. Leaf explants were irradiated with doses between 4 to 38 Gy of gamma rays, and cultured on somatic embryo induction medium. In addition, somatic embryos were irradiated with gamma ray doses from 10 to 18 Gy, and analyzed for germination. LD{sub 50} for embryogenic response of leaf-explants was at around 20 Gy, while that for somatic embryo germination was ca. 10 Gy. (author). 7 refs, 2 tabs.
Authors:
Lee, K S; Duren, M Van; Morpurgo, R [1] 
  1. Agriculture and Biotechnology Laboratory, International Atomic Energy Agency, Seibersdorf (Austria)
Publication Date:
Jul 01, 1997
Product Type:
Conference
Report Number:
IAEA-TECDOC-951; CONF-9510444-
Reference Number:
SCA: 553002; PA: AIX-28:054947; EDB-97:113821; SN: 97001834291
Resource Relation:
Conference: Final research coordination meeting on improvement of basic food crops ion Africa through plant breeding, including the use of induced mutations, Naples (Italy), 30 Oct 1995; Other Information: PBD: Jul 1997; Related Information: Is Part Of Improvement of basic food crops in Africa through plant breeding, including the use of induced mutations. Proceedings of a final research co-ordination meeting; PB: 145 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; CASSAVA; BIOLOGICAL REGENERATION; SOMATICALLY SIGNIFICANT DOSE; GERMINATION; MORPHOLOGICAL CHANGES; PLANT BREEDING; RADIATION DOSES; RADIATION INDUCED MUTANTS; SOMATIC MUTATIONS
OSTI ID:
511788
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE97637601; TRN: XA9744552054947
Availability:
INIS; OSTI as DE97637601
Submitting Site:
INIS
Size:
pp. 55-60
Announcement Date:

Citation Formats

Lee, K S, Duren, M Van, and Morpurgo, R. Somatic embryogenesis in cassava: A tool for mutation breeding. IAEA: N. p., 1997. Web.
Lee, K S, Duren, M Van, & Morpurgo, R. Somatic embryogenesis in cassava: A tool for mutation breeding. IAEA.
Lee, K S, Duren, M Van, and Morpurgo, R. 1997. "Somatic embryogenesis in cassava: A tool for mutation breeding." IAEA.
@misc{etde_511788,
title = {Somatic embryogenesis in cassava: A tool for mutation breeding}
author = {Lee, K S, Duren, M Van, and Morpurgo, R}
abstractNote = {Cassava is an important food and livestock feed crop. The effect of gamma radiation on somatic embryogenesis and plant regeneration in cassava clones of African origin was investigated. Explants from young leaves of cassava were cultured on MS medium, supplemented with 18.1 mM 2,4-D and 2 mM CuSO4, solidified with 0.3% Phytagel. Compact and friable calli were observed after 10-15 days of explant culture in dark, which produced somatic embryos in all but one clone. The somatic embryos showed morphological aberrations, such as fused cotyledons, lack of meristematic tip, epicotyl elongation, and had low germination rate; desiccation of embryos increased germination. Histological study showed that the somatic embryos were of multicellular origin. Leaf explants were irradiated with doses between 4 to 38 Gy of gamma rays, and cultured on somatic embryo induction medium. In addition, somatic embryos were irradiated with gamma ray doses from 10 to 18 Gy, and analyzed for germination. LD{sub 50} for embryogenic response of leaf-explants was at around 20 Gy, while that for somatic embryo germination was ca. 10 Gy. (author). 7 refs, 2 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}