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DNA analysis in three populations of African spinach (Basella spp.)

Abstract

African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - `Congo native`, `Cong domesticated`, and an introduced cultivar, `Sri Lanka` from Sri Lanka. Normal spinach (Spinacia oleracea) cv. `Prince F{sub 1} Hybrid` was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, `Cong domesticated` and `Sri Lanka` showed very low amount of genetic variation as revealed by RAPD analysis; the third population `Congo native` showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs.
Authors:
Grasso, G; Van Duren, M; Lee, K S; Morpurgo, R [1] 
  1. Agriculture and Biotechnology Lab., International Atomic Energy Agency, Seiberdorf (Austria)
Publication Date:
Jul 01, 1997
Product Type:
Conference
Report Number:
IAEA-TECDOC-951; CONF-9510444-
Reference Number:
SCA: 553002; PA: AIX-28:054945; EDB-97:113816; SN: 97001834289
Resource Relation:
Conference: Final research coordination meeting on improvement of basic food crops ion Africa through plant breeding, including the use of induced mutations, Naples (Italy), 30 Oct 1995; Other Information: PBD: Jul 1997; Related Information: Is Part Of Improvement of basic food crops in Africa through plant breeding, including the use of induced mutations. Proceedings of a final research co-ordination meeting; PB: 145 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; SPINACH; GENETIC VARIABILITY; AFRICA; BIOLOGICAL MARKERS; BROMIDES; CYTOLOGY; DNA SEQUENCING; PLOIDY
OSTI ID:
511786
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE97637601; TRN: XA9744550054945
Availability:
INIS; OSTI as DE97637601
Submitting Site:
INIS
Size:
pp. 39-43
Announcement Date:

Citation Formats

Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R. DNA analysis in three populations of African spinach (Basella spp.). IAEA: N. p., 1997. Web.
Grasso, G, Van Duren, M, Lee, K S, & Morpurgo, R. DNA analysis in three populations of African spinach (Basella spp.). IAEA.
Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R. 1997. "DNA analysis in three populations of African spinach (Basella spp.)." IAEA.
@misc{etde_511786,
title = {DNA analysis in three populations of African spinach (Basella spp.)}
author = {Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R}
abstractNote = {African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - `Congo native`, `Cong domesticated`, and an introduced cultivar, `Sri Lanka` from Sri Lanka. Normal spinach (Spinacia oleracea) cv. `Prince F{sub 1} Hybrid` was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, `Cong domesticated` and `Sri Lanka` showed very low amount of genetic variation as revealed by RAPD analysis; the third population `Congo native` showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}