Abstract
African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - `Congo native`, `Cong domesticated`, and an introduced cultivar, `Sri Lanka` from Sri Lanka. Normal spinach (Spinacia oleracea) cv. `Prince F{sub 1} Hybrid` was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, `Cong domesticated` and `Sri Lanka` showed very low amount of genetic variation as revealed by RAPD analysis; the third population `Congo native` showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs.
Grasso, G;
Van Duren, M;
Lee, K S;
Morpurgo, R
[1]
- Agriculture and Biotechnology Lab., International Atomic Energy Agency, Seiberdorf (Austria)
Citation Formats
Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R.
DNA analysis in three populations of African spinach (Basella spp.).
IAEA: N. p.,
1997.
Web.
Grasso, G, Van Duren, M, Lee, K S, & Morpurgo, R.
DNA analysis in three populations of African spinach (Basella spp.).
IAEA.
Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R.
1997.
"DNA analysis in three populations of African spinach (Basella spp.)."
IAEA.
@misc{etde_511786,
title = {DNA analysis in three populations of African spinach (Basella spp.)}
author = {Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R}
abstractNote = {African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - `Congo native`, `Cong domesticated`, and an introduced cultivar, `Sri Lanka` from Sri Lanka. Normal spinach (Spinacia oleracea) cv. `Prince F{sub 1} Hybrid` was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, `Cong domesticated` and `Sri Lanka` showed very low amount of genetic variation as revealed by RAPD analysis; the third population `Congo native` showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}
title = {DNA analysis in three populations of African spinach (Basella spp.)}
author = {Grasso, G, Van Duren, M, Lee, K S, and Morpurgo, R}
abstractNote = {African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - `Congo native`, `Cong domesticated`, and an introduced cultivar, `Sri Lanka` from Sri Lanka. Normal spinach (Spinacia oleracea) cv. `Prince F{sub 1} Hybrid` was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, `Cong domesticated` and `Sri Lanka` showed very low amount of genetic variation as revealed by RAPD analysis; the third population `Congo native` showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}