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Enzymic conversion of starch to glucose

Abstract

Corn is steeped in a SO/sub 2/ solution for 30 to 40 hours, coarsely ground, separated from the germ, and filtered. A 35% suspension of the germ-free corn, still containing fibers, hull, and gluten, is treated with Ca(OH)/sub 2/ to raise the pH to 6.5 to 7.0. A starch-liquifying enzyme is added and after a 2 hours treatment at 85/sup 0/ the liquefied starch is cooled to 60/sup 0/ and the pH is adjusted to 4.5 to 5.0 with H/sub 2/SO/sub 4/. A saccharifying enzyme is now added. After 40 to 81 hours, a raw glucose solution is obtained and is freed from fibers and gluten by filtration. The commercial starch-liquifying enzymes are designated HT-1000 and Neozyme 3 LC (liquid). The saccharifying enzymes are Diazyme or Diazyme L 30 (liquid). The solid enzymes are used at a level up to 0.1% by weight of the starch. Up to 100% conversion of starch into glucose is achieved.
Authors:
Publication Date:
Aug 19, 1964
Product Type:
Patent
Report Number:
NL 6401495
Reference Number:
EDB-80-116231
Resource Relation:
Patent File Date: Filed date 18 Feb 1963
Subject:
09 BIOMASS FUELS; GLUCOSE; BIOSYNTHESIS; MAIZE; ENZYMATIC HYDROLYSIS; STARCH; BIOCONVERSION; CALCIUM HYDROXIDES; COOLING; ENZYME ACTIVITY; ENZYMES; MEDIUM TEMPERATURE; PH VALUE; SULFUR DIOXIDE; SULFURIC ACID; SUSPENSIONS; ALDEHYDES; ALKALINE EARTH METAL COMPOUNDS; CALCIUM COMPOUNDS; CARBOHYDRATES; CEREALS; CHALCOGENIDES; CHEMICAL REACTIONS; DECOMPOSITION; DISPERSIONS; GRAMINEAE; GRASS; HEXOSES; HYDROGEN COMPOUNDS; HYDROLYSIS; HYDROXIDES; INORGANIC ACIDS; LYSIS; MONOSACCHARIDES; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SOLVOLYSIS; SULFUR COMPOUNDS; SULFUR OXIDES; SYNTHESIS; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
5113044
Country of Origin:
Netherlands
Language:
English
Submitting Site:
IEA
Size:
Pages: 13
Announcement Date:

Citation Formats

Not Available. Enzymic conversion of starch to glucose. Netherlands: N. p., 1964. Web.
Not Available. Enzymic conversion of starch to glucose. Netherlands.
Not Available. 1964. "Enzymic conversion of starch to glucose." Netherlands.
@misc{etde_5113044,
title = {Enzymic conversion of starch to glucose}
author = {Not Available}
abstractNote = {Corn is steeped in a SO/sub 2/ solution for 30 to 40 hours, coarsely ground, separated from the germ, and filtered. A 35% suspension of the germ-free corn, still containing fibers, hull, and gluten, is treated with Ca(OH)/sub 2/ to raise the pH to 6.5 to 7.0. A starch-liquifying enzyme is added and after a 2 hours treatment at 85/sup 0/ the liquefied starch is cooled to 60/sup 0/ and the pH is adjusted to 4.5 to 5.0 with H/sub 2/SO/sub 4/. A saccharifying enzyme is now added. After 40 to 81 hours, a raw glucose solution is obtained and is freed from fibers and gluten by filtration. The commercial starch-liquifying enzymes are designated HT-1000 and Neozyme 3 LC (liquid). The saccharifying enzymes are Diazyme or Diazyme L 30 (liquid). The solid enzymes are used at a level up to 0.1% by weight of the starch. Up to 100% conversion of starch into glucose is achieved.}
place = {Netherlands}
year = {1964}
month = {Aug}
}