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Increase of survival of x-irradiated mice by postirradiation injections of a splenic extract prepared from vaccine or endotoxin-treated syngeneic animals

Journal Article:

Abstract

Spleens of mice which had been treated with E. coli endotoxin or typhoid-paratyphoid vaccine were extracted with isotonic saline. The extract was filtered through an asbestos filter and chromatographed on a Sephadex G-200 column. The fraction which was excluded at around 2.5-void volume (molecular weight, about 20,000) was significantly effective in increasing survival of animals when it was repeatedly injected in mice after x irradiation (600 R). The injection caused an increase of the weight of spleen of the animals with an increased number of endogenous spleen colonies. The result suggests that the life-saving effect of the spleen extract is based on its effect of stimulating repopulation of autochthonous hematopoietic cells in the x-irradiated animals. A similar splenic extract prepared from normal mice had an ambiguous effect on the survival of x-irradiated animals.
Authors:
Tsuneoka, K; Takagi, Y; Shikita, M [1] 
  1. National Inst. of Radiological Sciences, Chiba (Japan)
Publication Date:
May 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-349755; EDB-78-070922
Resource Relation:
Journal Name: J. Radiat. Res.; (Japan); Journal Volume: 18:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MICE; SURVIVAL CURVES; TISSUE EXTRACTS; RADIOSENSITIVITY EFFECTS; CHEMICAL PREPARATION; ENDOTOXINS; POST-IRRADIATION THERAPY; RADIATION DOSES; RADIATION PROTECTION; SOLVENT EXTRACTION; SPLEEN; SPLEEN COLONY FORMATION; VACCINES; WHOLE-BODY IRRADIATION; X RADIATION; ANIMALS; ANTIGENS; BIOLOGICAL MATERIALS; BODY; COLONY FORMATION; DOSES; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; EXTRACTION; IONIZING RADIATIONS; IRRADIATION; MAMMALS; ORGANS; RADIATIONS; RODENTS; SEPARATION PROCESSES; SYNTHESIS; THERAPY; TISSUES; TOXIC MATERIALS; TOXINS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
5111840
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JRARA
Submitting Site:
INIS
Size:
Pages: 102-108
Announcement Date:
Jan 01, 1978

Journal Article:

Citation Formats

Tsuneoka, K, Takagi, Y, and Shikita, M. Increase of survival of x-irradiated mice by postirradiation injections of a splenic extract prepared from vaccine or endotoxin-treated syngeneic animals. Japan: N. p., 1977. Web.
Tsuneoka, K, Takagi, Y, & Shikita, M. Increase of survival of x-irradiated mice by postirradiation injections of a splenic extract prepared from vaccine or endotoxin-treated syngeneic animals. Japan.
Tsuneoka, K, Takagi, Y, and Shikita, M. 1977. "Increase of survival of x-irradiated mice by postirradiation injections of a splenic extract prepared from vaccine or endotoxin-treated syngeneic animals." Japan.
@misc{etde_5111840,
title = {Increase of survival of x-irradiated mice by postirradiation injections of a splenic extract prepared from vaccine or endotoxin-treated syngeneic animals}
author = {Tsuneoka, K, Takagi, Y, and Shikita, M}
abstractNote = {Spleens of mice which had been treated with E. coli endotoxin or typhoid-paratyphoid vaccine were extracted with isotonic saline. The extract was filtered through an asbestos filter and chromatographed on a Sephadex G-200 column. The fraction which was excluded at around 2.5-void volume (molecular weight, about 20,000) was significantly effective in increasing survival of animals when it was repeatedly injected in mice after x irradiation (600 R). The injection caused an increase of the weight of spleen of the animals with an increased number of endogenous spleen colonies. The result suggests that the life-saving effect of the spleen extract is based on its effect of stimulating repopulation of autochthonous hematopoietic cells in the x-irradiated animals. A similar splenic extract prepared from normal mice had an ambiguous effect on the survival of x-irradiated animals.}
journal = {J. Radiat. Res.; (Japan)}
volume = {18:2}
journal type = {AC}
place = {Japan}
year = {1977}
month = {May}
}