You need JavaScript to view this

Mechanism of mercuric chloride resistance in microorganisms. II. NADPH-dependent reduction of mercuric chloride and vaporization of mercury from mercuric chloride by a multiple drug resistant strain of Escherichia coli

Journal Article:

Abstract

The activity to vaporize a /sup 203/Hg compound from /sup 203/HgCl/sub 2/ was demonstrated in crude cell-free extracts of a strain of Escherichia coli W2252, which had acquired the multiple drug resistance. NADPH was essential for the vaporization, while NADH had only a slight stimulating effect and NADP/sup +/ had no effect. The oxidation of NADPH dependent on HgCl/sub 2/ was also demonstrated in the crude extracts, but the HgCl/sub 2/-dependent NADH oxidation could be demonstrated only when a partially purified enzyme preparation was used. The rate of NADH oxidation was much slower than that of NADPH oxidation. It was concluded that NADPH, and to a lesser extent NADH, act as electron donors for the enzymatic reduction of HgCl/sub 2/ and the vaporization occurs after this reduction. This reduction and subsequent vaporization seem to provide a mechanism of resistance to HgCl/sub 2/ in E. coli strains having the multiple drug resistance. 15 references, 4 figures, 4 tables.
Publication Date:
Jan 01, 1971
Product Type:
Journal Article
Reference Number:
EDB-85-177250
Resource Relation:
Journal Name: J. Biochem. (Tokyo); (Japan); Journal Volume: 70
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; SENSITIVITY; MERCURY; EVAPORATION; METABOLISM; NADH2; OXIDATION; NADP; EXPERIMENTAL DATA; LABELLED COMPOUNDS; MERCURY 203; TRACER TECHNIQUES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHEMICAL REACTIONS; COENZYMES; DATA; DAYS LIVING RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; HEAVY NUCLEI; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; MERCURY ISOTOPES; METALS; MICROORGANISMS; NUCLEI; NUCLEOTIDES; NUMERICAL DATA; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; RADIOISOTOPES; 550501* - Metabolism- Tracer Techniques; 560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
OSTI ID:
5097262
Research Organizations:
Tohoku Univ., Sendai, Japan
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JOBIA
Submitting Site:
HEDB
Size:
Pages: 895-901
Announcement Date:

Journal Article:

Citation Formats

Komura, I, Funaba, T, and Izaki, K. Mechanism of mercuric chloride resistance in microorganisms. II. NADPH-dependent reduction of mercuric chloride and vaporization of mercury from mercuric chloride by a multiple drug resistant strain of Escherichia coli. Japan: N. p., 1971. Web.
Komura, I, Funaba, T, & Izaki, K. Mechanism of mercuric chloride resistance in microorganisms. II. NADPH-dependent reduction of mercuric chloride and vaporization of mercury from mercuric chloride by a multiple drug resistant strain of Escherichia coli. Japan.
Komura, I, Funaba, T, and Izaki, K. 1971. "Mechanism of mercuric chloride resistance in microorganisms. II. NADPH-dependent reduction of mercuric chloride and vaporization of mercury from mercuric chloride by a multiple drug resistant strain of Escherichia coli." Japan.
@misc{etde_5097262,
title = {Mechanism of mercuric chloride resistance in microorganisms. II. NADPH-dependent reduction of mercuric chloride and vaporization of mercury from mercuric chloride by a multiple drug resistant strain of Escherichia coli}
author = {Komura, I, Funaba, T, and Izaki, K}
abstractNote = {The activity to vaporize a /sup 203/Hg compound from /sup 203/HgCl/sub 2/ was demonstrated in crude cell-free extracts of a strain of Escherichia coli W2252, which had acquired the multiple drug resistance. NADPH was essential for the vaporization, while NADH had only a slight stimulating effect and NADP/sup +/ had no effect. The oxidation of NADPH dependent on HgCl/sub 2/ was also demonstrated in the crude extracts, but the HgCl/sub 2/-dependent NADH oxidation could be demonstrated only when a partially purified enzyme preparation was used. The rate of NADH oxidation was much slower than that of NADPH oxidation. It was concluded that NADPH, and to a lesser extent NADH, act as electron donors for the enzymatic reduction of HgCl/sub 2/ and the vaporization occurs after this reduction. This reduction and subsequent vaporization seem to provide a mechanism of resistance to HgCl/sub 2/ in E. coli strains having the multiple drug resistance. 15 references, 4 figures, 4 tables.}
journal = {J. Biochem. (Tokyo); (Japan)}
volume = {70}
journal type = {AC}
place = {Japan}
year = {1971}
month = {Jan}
}