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Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects

Journal Article:

Abstract

We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.
Authors:
Pradalier, N; Canal, P; Pujol, A; Fregevu, Y; [1]  Soula, G [2] 
  1. Groupe de Recherches du Centre Claudius-Regaud, Toulouse (France)
  2. Faculte des Sciences Pharmaceutiques, Toulouse (France)
Publication Date:
Jan 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-13-687008; EDB-82-152834
Resource Relation:
Journal Name: Ann. Biol. Clin. (Paris); (France); Journal Volume: 40:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ACID PHOSPHATASE; RADIOIMMUNOASSAY; PROSTATE; NEOPLASMS; BLOOD SERUM; CALIBRATION STANDARDS; EXPERIMENTAL DATA; IMMUNE SERUMS; IODINE 125; BETA DECAY RADIOISOTOPES; BODY; DATA; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; ESTERASES; GLANDS; HYDROLASES; INFORMATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MALE GENITALS; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANS; PHOSPHATASES; RADIOASSAY; RADIOISOTOPES; STANDARDS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5077691
Country of Origin:
France
Language:
French
Other Identifying Numbers:
Journal ID: CODEN: ABCLA
Submitting Site:
HEDB
Size:
Pages: 45-52
Announcement Date:

Journal Article:

Citation Formats

Pradalier, N, Canal, P, Pujol, A, Fregevu, Y, and Soula, G. Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects. France: N. p., 1982. Web.
Pradalier, N, Canal, P, Pujol, A, Fregevu, Y, & Soula, G. Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects. France.
Pradalier, N, Canal, P, Pujol, A, Fregevu, Y, and Soula, G. 1982. "Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects." France.
@misc{etde_5077691,
title = {Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects}
author = {Pradalier, N, Canal, P, Pujol, A, Fregevu, Y, and Soula, G}
abstractNote = {We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.}
journal = {Ann. Biol. Clin. (Paris); (France)}
volume = {40:1}
journal type = {AC}
place = {France}
year = {1982}
month = {Jan}
}