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Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose

Journal Article:

Abstract

The enzymic conversion of cellulose is catalyzed by a multiple enzyme system. The Trichoderma enzyme system has insufficient ..beta..-glucosidase (EC 3.2.1.21) activity for the practical saccharification of cellulose. Aspergillus niger and A. phoenicis were superior producers of ..beta.. glucosidase and a method for production of this enzyme in liquid culture is presented. When Trichoderma cellulase preparations are supplemented with ..beta.. glucosidase from Aspergullus during practical saccharifications glucose is the predominant product and the rate of saccharification is significantly increased. The stimulatory effect of ..beta.. glucosidase appears to be due to the removal of inhibitory levels of cellobiose.
Authors:
Publication Date:
Jan 01, 1977
Product Type:
Journal Article
Reference Number:
EDB-80-124790
Resource Relation:
Journal Name: Can. J. Microbiol.; (Canada); Journal Volume: 23:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; CELLULOSE; ENZYMATIC HYDROLYSIS; SYNERGISM; GLUCOSE; BIOSYNTHESIS; ASPERGILLUS; CELLOBIOSE; CELLULASES; ENZYMES; TRICHODERMA; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DISACCHARIDES; FUNGI; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; SYNTHESIS; 550700* - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550200 - Biochemistry; 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
OSTI ID:
5072702
Research Organizations:
US Army Natick Res. Dev. Command, MA
Country of Origin:
Canada
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CJMIA
Submitting Site:
IEA
Size:
Pages: 139-147
Announcement Date:

Journal Article:

Citation Formats

Sternberg, D. Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose. Canada: N. p., 1977. Web.
Sternberg, D. Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose. Canada.
Sternberg, D. 1977. "Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose." Canada.
@misc{etde_5072702,
title = {Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose}
author = {Sternberg, D}
abstractNote = {The enzymic conversion of cellulose is catalyzed by a multiple enzyme system. The Trichoderma enzyme system has insufficient ..beta..-glucosidase (EC 3.2.1.21) activity for the practical saccharification of cellulose. Aspergillus niger and A. phoenicis were superior producers of ..beta.. glucosidase and a method for production of this enzyme in liquid culture is presented. When Trichoderma cellulase preparations are supplemented with ..beta.. glucosidase from Aspergullus during practical saccharifications glucose is the predominant product and the rate of saccharification is significantly increased. The stimulatory effect of ..beta.. glucosidase appears to be due to the removal of inhibitory levels of cellobiose.}
journal = {Can. J. Microbiol.; (Canada)}
volume = {23:2}
journal type = {AC}
place = {Canada}
year = {1977}
month = {Jan}
}