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Dendritic and axonic fields of Purkinje cells in developing and X-irradiated rat cerebellum. A comparative study using intracellular staining with horseradish peroxidase

Abstract

Intracellular staining of cerebellar Purkinje cells with horseradish peroxidase was achieved in normal developing rats (8-13 days old), in normal adult rats and in adult rats in which the cerebellum had been degranulated by X-ray treatment. The mono- and multiple innervation of Purkinje cells by climbing fibres was electrophysiologically determined and correlated with their dendritic pattern and axonal field. In immature rats, considerable variations in dendritic arborization were observed between cells at the same age, according to their position in the vermis. In adult X-irradiated animals, a large variety of dendritic shapes was found, confirming previous anatomical data, but no obvious correlation was found between the morphology of the dendrites of Purkinje cells and their synaptic investment by climbing fibres. As regards the axonal field, the adult branching pattern of recurrent axon collaterals was almost established by postnatal day 8, except for some cells which exhibited richer recurrent collaterals. On the other hand, in X-irradiated animals, profuse plexuses were the rule and they originated either from one collateral stem, or from several collaterals, also independently of the number of afferent climbing fibres. The existence of these enlarged recurrent collateral plexuses can be explained by the persistence of an immature stage,  More>>
Authors:
Crepel, F; Delhaye-Bouchaud, N; Dupont, J L; [1]  Sotelo, C [2] 
  1. Paris-5 Univ., 75 (France)
  2. Hopital Foch, 92 - Suresnes (France). Centre Medico-Chirurgical
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-541051; EDB-80-124851
Resource Relation:
Journal Name: Neuroscience; (United Kingdom); Journal Volume: 5:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; NERVE CELLS; GROWTH; X RADIATION; BIOLOGICAL RADIATION EFFECTS; ADULTS; AGE DEPENDENCE; CELL CONSTITUENTS; CEREBELLUM; FIBERS; IRRADIATION; MICROSCOPY; MORPHOLOGICAL CHANGES; NEONATES; PEROXIDASES; RATS; STAINS; AGE GROUPS; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BODY; BRAIN; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IONIZING RADIATIONS; MAMMALS; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXIDOREDUCTASES; PORPHYRINS; RADIATION EFFECTS; RADIATIONS; RODENTS; SOMATIC CELLS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5072492
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NRSCD
Submitting Site:
INIS
Size:
Pages: 333-347
Announcement Date:

Citation Formats

Crepel, F, Delhaye-Bouchaud, N, Dupont, J L, and Sotelo, C. Dendritic and axonic fields of Purkinje cells in developing and X-irradiated rat cerebellum. A comparative study using intracellular staining with horseradish peroxidase. United Kingdom: N. p., 1980. Web. doi:10.1016/0306-4522(80)90109-8.
Crepel, F, Delhaye-Bouchaud, N, Dupont, J L, & Sotelo, C. Dendritic and axonic fields of Purkinje cells in developing and X-irradiated rat cerebellum. A comparative study using intracellular staining with horseradish peroxidase. United Kingdom. doi:10.1016/0306-4522(80)90109-8.
Crepel, F, Delhaye-Bouchaud, N, Dupont, J L, and Sotelo, C. 1980. "Dendritic and axonic fields of Purkinje cells in developing and X-irradiated rat cerebellum. A comparative study using intracellular staining with horseradish peroxidase." United Kingdom. doi:10.1016/0306-4522(80)90109-8. https://www.osti.gov/servlets/purl/10.1016/0306-4522(80)90109-8.
@misc{etde_5072492,
title = {Dendritic and axonic fields of Purkinje cells in developing and X-irradiated rat cerebellum. A comparative study using intracellular staining with horseradish peroxidase}
author = {Crepel, F, Delhaye-Bouchaud, N, Dupont, J L, and Sotelo, C}
abstractNote = {Intracellular staining of cerebellar Purkinje cells with horseradish peroxidase was achieved in normal developing rats (8-13 days old), in normal adult rats and in adult rats in which the cerebellum had been degranulated by X-ray treatment. The mono- and multiple innervation of Purkinje cells by climbing fibres was electrophysiologically determined and correlated with their dendritic pattern and axonal field. In immature rats, considerable variations in dendritic arborization were observed between cells at the same age, according to their position in the vermis. In adult X-irradiated animals, a large variety of dendritic shapes was found, confirming previous anatomical data, but no obvious correlation was found between the morphology of the dendrites of Purkinje cells and their synaptic investment by climbing fibres. As regards the axonal field, the adult branching pattern of recurrent axon collaterals was almost established by postnatal day 8, except for some cells which exhibited richer recurrent collaterals. On the other hand, in X-irradiated animals, profuse plexuses were the rule and they originated either from one collateral stem, or from several collaterals, also independently of the number of afferent climbing fibres. The existence of these enlarged recurrent collateral plexuses can be explained by the persistence of an immature stage, and certainly also by the collateral sprouting following the largely impaired innervation of the terminal field during development. These results emphasize the role of the cellular interactions that occur during Purkinje cell growth in the formation of both its axonal and dendritic fields.}
doi = {10.1016/0306-4522(80)90109-8}
journal = {Neuroscience; (United Kingdom)}
volume = {5:2}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {Jan}
}