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Acquisition of useful and high ability genes for acidophilic bacteria; Kosansei saikin ni takai noryoku wo fuyosuru idenshi no kakutoku

Abstract

This effort aims at the development of high-performance bacteria usable in bio-leaching in metal smelting by acquiring genes capable of realizing such. A method is used of choosing some isolated strains exhibiting high-performance traits and acquiring target genes therefrom by use of genetic engineering. Approximately 200 kinds in the aggregate of acidophilic bacteria are currently available for the study, including isolated iron-oxidizing and sulfur-oxidizing bacteria, standard species acquired for the study, and strains previously isolated by the laboratory. The bacteria are tested with respect to their Fe{sup 2+}-oxidizing rates, sulfur-oxidizing capabilities, and strength to withstand inhibiting substances (Ag{sup +}, Cl{sup -}, Mo{sup 6+}, etc.), which results in the nomination of 8 strains. The study planned to follow includes processes involving the extraction of chromosome DNAs from the 8 strains and their refinement, gene cloning by the Southern hybridization method, determination of their base sequences, determination of the difference between the strains in point of gene expression, and investigations of the relations that the results of these processes bear toward the said high-performance traits. Also under way is a study about the infuence-exerting factors revealed during the evaluation of the abilities of acidphlic bacteria. 2 refs., 2 tabs.
Authors:
Senda, T; Inoue, C; Shinbori, Y [1] 
  1. Tohoku University, Sendai (Japan)
Publication Date:
Feb 01, 1997
Product Type:
Conference
Report Number:
CONF-960283-
Reference Number:
SCA: 360101; 550400; 550700; PA: NEDO-97:820046; EDB-97:107301; SN: 97001821140
Resource Relation:
Conference: Group of scientific experts (GSE) working group, 1995 nendo teian kobogata saisentan (juten) bun`ya kenkyu kaihatsu seika hokokukai, Geneva (Switzerland), 12-23 Feb 1996; Other Information: PBD: Feb 1997; Related Information: Is Part Of Preprint of the results of `the publicly applied proposal type and hi-tech (emphasized) field research and development in fiscal 1995`; PB: 469 p.; `1995 nendo teian kobogata saisentan (juten) bun`ya kenkyu kaihatsu` seika hokokukai yokoshu
Subject:
36 MATERIALS SCIENCE; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; THIOBACILLUS FERROXIDANS; GENES; METALLURGY; LEACHING; BIOTECHNOLOGY; SULFUR-OXIDIZING BACTERIA; HAZARDOUS MATERIALS; CHROMOSOMES; DNA; HYBRIDIZATION; DNA-CLONING
OSTI ID:
506684
Research Organizations:
New Energy and Industrial Technology Development Organization, Tokyo (Japan)
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Other: ON: DE97757283; TRN: 97:820046
Availability:
Available from New Energy and Industrial Technology Development Organization, Sunshine 60, 30F 1-1, 3-chome, Higashi-Ikebukuro, Toshima-ku, Tokyo, Japan; OSTI as DE97757283
Submitting Site:
NEDO
Size:
pp. 410-411
Announcement Date:

Citation Formats

Senda, T, Inoue, C, and Shinbori, Y. Acquisition of useful and high ability genes for acidophilic bacteria; Kosansei saikin ni takai noryoku wo fuyosuru idenshi no kakutoku. Japan: N. p., 1997. Web.
Senda, T, Inoue, C, & Shinbori, Y. Acquisition of useful and high ability genes for acidophilic bacteria; Kosansei saikin ni takai noryoku wo fuyosuru idenshi no kakutoku. Japan.
Senda, T, Inoue, C, and Shinbori, Y. 1997. "Acquisition of useful and high ability genes for acidophilic bacteria; Kosansei saikin ni takai noryoku wo fuyosuru idenshi no kakutoku." Japan.
@misc{etde_506684,
title = {Acquisition of useful and high ability genes for acidophilic bacteria; Kosansei saikin ni takai noryoku wo fuyosuru idenshi no kakutoku}
author = {Senda, T, Inoue, C, and Shinbori, Y}
abstractNote = {This effort aims at the development of high-performance bacteria usable in bio-leaching in metal smelting by acquiring genes capable of realizing such. A method is used of choosing some isolated strains exhibiting high-performance traits and acquiring target genes therefrom by use of genetic engineering. Approximately 200 kinds in the aggregate of acidophilic bacteria are currently available for the study, including isolated iron-oxidizing and sulfur-oxidizing bacteria, standard species acquired for the study, and strains previously isolated by the laboratory. The bacteria are tested with respect to their Fe{sup 2+}-oxidizing rates, sulfur-oxidizing capabilities, and strength to withstand inhibiting substances (Ag{sup +}, Cl{sup -}, Mo{sup 6+}, etc.), which results in the nomination of 8 strains. The study planned to follow includes processes involving the extraction of chromosome DNAs from the 8 strains and their refinement, gene cloning by the Southern hybridization method, determination of their base sequences, determination of the difference between the strains in point of gene expression, and investigations of the relations that the results of these processes bear toward the said high-performance traits. Also under way is a study about the infuence-exerting factors revealed during the evaluation of the abilities of acidphlic bacteria. 2 refs., 2 tabs.}
place = {Japan}
year = {1997}
month = {Feb}
}