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Quantification of methanogenic biomass by enzyme-linked immunosorbent assay and by analysis of specific methanogenic cofactors

Abstract

The reliability and accuracy with which enzyme-linked immunosorbent assay (ELISA) and an assay of methanogenic cofactors detect and quantify methanogenic species were investigated. Both assays required standardization with laboratory cultures of methanogenic bacteria and were applied to mixtures of pure cultures and samples from anaerobic digesters. ELISA was shown to be a simple method for detecting and quantifying individual methanogenic species. The range of species which can be assayed is limited by the range of antisera available but, potentially, ELISA can be applied to all methanogens. Although the cofactor assay is not species-specific it can distinguish hydrogenotrophic and acetotrophic methanogens and is quantitative.
Publication Date:
Jan 01, 1987
Product Type:
Journal Article
Reference Number:
GB-88-000679; EDB-88-112022
Resource Relation:
Journal Name: Biomass; (United Kingdom); Journal Volume: 14:3
Subject:
09 BIOMASS FUELS; BIOGAS PROCESS; ENZYME IMMUNOASSAY; QUANTITATIVE CHEMICAL ANALYSIS; ACCURACY; METHANOGENIC BACTERIA; PROCESS CONTROL; PURIFICATION; RELIABILITY; WASTE WATER; ANAEROBIC DIGESTION; BACTERIA; BIOCONVERSION; CHEMICAL ANALYSIS; CONTROL; DIGESTION; HYDROGEN COMPOUNDS; IMMUNOASSAY; LIQUID WASTES; MANAGEMENT; MICROORGANISMS; OXYGEN COMPOUNDS; PROCESSING; WASTE MANAGEMENT; WASTE PROCESSING; WASTES; WATER; 090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)
OSTI ID:
5047627
Research Organizations:
Nijmegen Univ. (NL). Dept. of Microbiology; AFRC Inst. of Food Research, Norwich Lab. (GB)
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BIOME
Submitting Site:
GB
Size:
Pages: 195-208
Announcement Date:

Citation Formats

Gorris, L G.M., Kemp, H A, and Archer, D B. Quantification of methanogenic biomass by enzyme-linked immunosorbent assay and by analysis of specific methanogenic cofactors. United Kingdom: N. p., 1987. Web. doi:10.1016/0144-4565(87)90047-3.
Gorris, L G.M., Kemp, H A, & Archer, D B. Quantification of methanogenic biomass by enzyme-linked immunosorbent assay and by analysis of specific methanogenic cofactors. United Kingdom. doi:10.1016/0144-4565(87)90047-3.
Gorris, L G.M., Kemp, H A, and Archer, D B. 1987. "Quantification of methanogenic biomass by enzyme-linked immunosorbent assay and by analysis of specific methanogenic cofactors." United Kingdom. doi:10.1016/0144-4565(87)90047-3. https://www.osti.gov/servlets/purl/10.1016/0144-4565(87)90047-3.
@misc{etde_5047627,
title = {Quantification of methanogenic biomass by enzyme-linked immunosorbent assay and by analysis of specific methanogenic cofactors}
author = {Gorris, L G.M., Kemp, H A, and Archer, D B}
abstractNote = {The reliability and accuracy with which enzyme-linked immunosorbent assay (ELISA) and an assay of methanogenic cofactors detect and quantify methanogenic species were investigated. Both assays required standardization with laboratory cultures of methanogenic bacteria and were applied to mixtures of pure cultures and samples from anaerobic digesters. ELISA was shown to be a simple method for detecting and quantifying individual methanogenic species. The range of species which can be assayed is limited by the range of antisera available but, potentially, ELISA can be applied to all methanogens. Although the cofactor assay is not species-specific it can distinguish hydrogenotrophic and acetotrophic methanogens and is quantitative.}
doi = {10.1016/0144-4565(87)90047-3}
journal = {Biomass; (United Kingdom)}
volume = {14:3}
journal type = {AC}
place = {United Kingdom}
year = {1987}
month = {Jan}
}