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Localization of lead within leaf cells of Rhytidiadelphus squarrosus (Hedw. ) Warnst. by means of transmission electron microscopy and X-ray microanalysis

Abstract

Results of ultrastructural studies and transmission electron microscope microanalysis of leaves of the bryophyte Rhytidiadelphus squarrosus collected from a park in Trondheim are presented. The lead content of these leaves primarily derives from motor traffic exhaust gases. A fine structural examination of the leaf cells revealed that detectable amounts of lead had entered the cytoplasm and could be recognized as electron-dense precipitates localized inside the plasma membrane, within vesicles or vacuoles, chloroplasts, mitochondria, microbodies and plasmodesmata. Control material, fixed only in glutaraledhyde and not post-stained, showed that these precipitates must be due to metallic elements having great electron-scattering properties. TEM-X-ray microanalysis indicated the definite presence of lead and phosphorus within both the nuclear and chloroplast inclusions. The possible presence of some other metals is also discussed.
Publication Date:
Jan 01, 1974
Product Type:
Journal Article
Reference Number:
EDB-85-185538
Resource Relation:
Journal Name: Cytobios; (United Kingdom); Journal Volume: 10:37/38
Subject:
54 ENVIRONMENTAL SCIENCES; LEAD; BIOLOGICAL LOCALIZATION; PHOSPHORUS; AIR POLLUTION; AUTOMOBILES; BIOLOGICAL ACCUMULATION; CELL MEMBRANES; CHLOROPLASTS; ELECTRON MICROSCOPY; EXHAUST GASES; LEAVES; MITOCHONDRIA; MOSSES; NORWAY; X-RAY SPECTROSCOPY; CELL CONSTITUENTS; ELEMENTS; EUROPE; FLUIDS; GASEOUS WASTES; GASES; MEMBRANES; METALS; MICROSCOPY; NONMETALS; ORGANOIDS; PLANTS; POLLUTION; SCANDINAVIA; SPECTROSCOPY; VEHICLES; WASTES; WESTERN EUROPE; 510200* - Environment, Terrestrial- Chemicals Monitoring & Transport- (-1989); 500200 - Environment, Atmospheric- Chemicals Monitoring & Transport- (-1989)
OSTI ID:
5032233
Research Organizations:
Trondheim Univ., Norway
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CYTBA
Submitting Site:
HEDB
Size:
Pages: 45-48
Announcement Date:
Dec 01, 1985

Citation Formats

Ophus, E M, and Gullvag, B M. Localization of lead within leaf cells of Rhytidiadelphus squarrosus (Hedw. ) Warnst. by means of transmission electron microscopy and X-ray microanalysis. United Kingdom: N. p., 1974. Web.
Ophus, E M, & Gullvag, B M. Localization of lead within leaf cells of Rhytidiadelphus squarrosus (Hedw. ) Warnst. by means of transmission electron microscopy and X-ray microanalysis. United Kingdom.
Ophus, E M, and Gullvag, B M. 1974. "Localization of lead within leaf cells of Rhytidiadelphus squarrosus (Hedw. ) Warnst. by means of transmission electron microscopy and X-ray microanalysis." United Kingdom.
@misc{etde_5032233,
title = {Localization of lead within leaf cells of Rhytidiadelphus squarrosus (Hedw. ) Warnst. by means of transmission electron microscopy and X-ray microanalysis}
author = {Ophus, E M, and Gullvag, B M}
abstractNote = {Results of ultrastructural studies and transmission electron microscope microanalysis of leaves of the bryophyte Rhytidiadelphus squarrosus collected from a park in Trondheim are presented. The lead content of these leaves primarily derives from motor traffic exhaust gases. A fine structural examination of the leaf cells revealed that detectable amounts of lead had entered the cytoplasm and could be recognized as electron-dense precipitates localized inside the plasma membrane, within vesicles or vacuoles, chloroplasts, mitochondria, microbodies and plasmodesmata. Control material, fixed only in glutaraledhyde and not post-stained, showed that these precipitates must be due to metallic elements having great electron-scattering properties. TEM-X-ray microanalysis indicated the definite presence of lead and phosphorus within both the nuclear and chloroplast inclusions. The possible presence of some other metals is also discussed.}
journal = []
volume = {10:37/38}
journal type = {AC}
place = {United Kingdom}
year = {1974}
month = {Jan}
}