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Antigen spot test (AST): a highly sensitive assay for the detection of antibodies

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Abstract

A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.
Authors:
Herbrink, P; van Bussel, F J; Warnaar, S O [1] 
  1. Rijksuniversiteit Leiden (Netherlands)
Publication Date:
Feb 12, 1982
Product Type:
Journal Article
Reference Number:
AIX-13-677867; EDB-82-146282
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 48:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; RADIOASSAY; ANTIGENS; DIAZO COMPOUNDS; IODINE 125; LABELLED COMPOUNDS; NITROCELLULOSE; PROTEINS; SENSITIVITY; SPECIFICITY; BETA DECAY RADIOISOTOPES; CARBOHYDRATES; CELLULOSE ESTERS; CHEMICAL EXPLOSIVES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ESTERS; EXPLOSIVES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NITRIC ACID ESTERS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYSACCHARIDES; RADIOISOTOPES; SACCHARIDES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5031437
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 293-298
Announcement Date:
May 01, 1982

Journal Article:

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Citation Formats

Herbrink, P, van Bussel, F J, and Warnaar, S O. Antigen spot test (AST): a highly sensitive assay for the detection of antibodies. Netherlands: N. p., 1982. Web.
Herbrink, P, van Bussel, F J, & Warnaar, S O. Antigen spot test (AST): a highly sensitive assay for the detection of antibodies. Netherlands.
Herbrink, P, van Bussel, F J, and Warnaar, S O. 1982. "Antigen spot test (AST): a highly sensitive assay for the detection of antibodies." Netherlands.
@misc{etde_5031437,
title = {Antigen spot test (AST): a highly sensitive assay for the detection of antibodies}
 author = {Herbrink, P, van Bussel, F J, and Warnaar, S O}
 abstractNote = {A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.}
 journal = []
 volume = {48:3}
 journal type = {AC}
 place = {Netherlands}
 year = {1982}
 month = {Feb}
 }