Abstract
A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.
Citation Formats
Herbrink, P, van Bussel, F J, and Warnaar, S O.
Antigen spot test (AST): a highly sensitive assay for the detection of antibodies.
Netherlands: N. p.,
1982.
Web.
Herbrink, P, van Bussel, F J, & Warnaar, S O.
Antigen spot test (AST): a highly sensitive assay for the detection of antibodies.
Netherlands.
Herbrink, P, van Bussel, F J, and Warnaar, S O.
1982.
"Antigen spot test (AST): a highly sensitive assay for the detection of antibodies."
Netherlands.
@misc{etde_5031437,
title = {Antigen spot test (AST): a highly sensitive assay for the detection of antibodies}
author = {Herbrink, P, van Bussel, F J, and Warnaar, S O}
abstractNote = {A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {48:3}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Feb}
}
title = {Antigen spot test (AST): a highly sensitive assay for the detection of antibodies}
author = {Herbrink, P, van Bussel, F J, and Warnaar, S O}
abstractNote = {A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {48:3}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Feb}
}