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Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide

Journal Article:

Abstract

The effects of /sup 60/Co irradiation performed with various doses on the biological and chemical properties of the endotoxin of the Salmonella minnesota R595 were compared with those of unirradiated ones. The biological activity was measured using the lethal toxicity test, the local Schwartzman reaction and by activating the complementary system. Increasing the irradiation dose from 50 to 200 kGy the preparation became less active in the biological tests but the protective activity against the lethal action of the endotoxin remained uneffected. The irradiation resulted in a dose-dependent decrease of the amounts of 2-keto-3-deoxy-octonate, glucosamine, fatty acids, but did not affect all the degradation products identified. Therefore, no correlation between the chemical composition and the absence of endotoxin activity was found.
Authors:
El Sabbagh, M; Galanos, C; Luederitz, O; [1]  Bertok, L; [2]  Fuest, Gy [3] 
  1. Max-Planck-Institut fuer Immunbiologie, Freiburg (Germany, F.R.)
  2. Orszagos Frederic Joliot-Curie Sugarbiologiai es Sugaregeszseguegyi Kutato Intezet, Budapest (Hungary)
  3. Orszagos Haema--tologiai es Vertranszfuzios Intezet, Budapest (Hungary)
Publication Date:
Jan 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-15-005770; EDB-84-066969
Resource Relation:
Journal Name: Acta Microbiol. Acad. Sci. Hung.; (Hungary); Journal Volume: 29:4; Other Information: 24 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ENDOTOXINS; BIOLOGICAL RADIATION EFFECTS; LIPOPOLYSACCHARIDES; RADIOLYSIS; COBALT 60; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; SALMONELLA; ANTIGENS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CARBOHYDRATES; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; CHEMISTRY; COBALT ISOTOPES; DECOMPOSITION; ELECTROMAGNETIC RADIATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LIPIDS; MATERIALS; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; POLYSACCHARIDES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SACCHARIDES; TOXIC MATERIALS; TOXINS; YEARS LIVING RADIOISOTOPES; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
5020174
Country of Origin:
Hungary
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AMAHA
Submitting Site:
HEDB
Size:
Pages: 255-261
Announcement Date:

Journal Article:

Citation Formats

El Sabbagh, M, Galanos, C, Luederitz, O, Bertok, L, and Fuest, Gy. Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide. Hungary: N. p., 1982. Web.
El Sabbagh, M, Galanos, C, Luederitz, O, Bertok, L, & Fuest, Gy. Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide. Hungary.
El Sabbagh, M, Galanos, C, Luederitz, O, Bertok, L, and Fuest, Gy. 1982. "Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide." Hungary.
@misc{etde_5020174,
title = {Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide}
author = {El Sabbagh, M, Galanos, C, Luederitz, O, Bertok, L, and Fuest, Gy}
abstractNote = {The effects of /sup 60/Co irradiation performed with various doses on the biological and chemical properties of the endotoxin of the Salmonella minnesota R595 were compared with those of unirradiated ones. The biological activity was measured using the lethal toxicity test, the local Schwartzman reaction and by activating the complementary system. Increasing the irradiation dose from 50 to 200 kGy the preparation became less active in the biological tests but the protective activity against the lethal action of the endotoxin remained uneffected. The irradiation resulted in a dose-dependent decrease of the amounts of 2-keto-3-deoxy-octonate, glucosamine, fatty acids, but did not affect all the degradation products identified. Therefore, no correlation between the chemical composition and the absence of endotoxin activity was found.}
journal = {Acta Microbiol. Acad. Sci. Hung.; (Hungary)}
volume = {29:4}
journal type = {AC}
place = {Hungary}
year = {1982}
month = {Jan}
}