Abstract
Blood and serum samples from cattle originating from tsetse free and tsetse infested areas were analyzed using the Buffy Coat technique and an ELISA to detect trypanosomes and trypanosomal antigens, respectively. The results of the two tests were compared and apparent sensitivity and trypanosomal prevalence were calculated. The BCT seemed to be the most suitable test to detect acute infections, while the antigen capture ELISA (Ag-ELISA) was able to detect more chronic infections. The specificity of the Ag-ELISA was found to be very good, but the sensitivity of the test should be improved. One way to detect more T. congolense and T. vivax infections was to lower the cut-off point of percent positivity from 10 to 5%. (author). 1 ref., 1 fig., 7 tabs.
Citation Formats
Ries, R, and Nqindi, J.
The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe.
IAEA: N. p.,
1997.
Web.
Ries, R, & Nqindi, J.
The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe.
IAEA.
Ries, R, and Nqindi, J.
1997.
"The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe."
IAEA.
@misc{etde_480990,
title = {The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe}
author = {Ries, R, and Nqindi, J}
abstractNote = {Blood and serum samples from cattle originating from tsetse free and tsetse infested areas were analyzed using the Buffy Coat technique and an ELISA to detect trypanosomes and trypanosomal antigens, respectively. The results of the two tests were compared and apparent sensitivity and trypanosomal prevalence were calculated. The BCT seemed to be the most suitable test to detect acute infections, while the antigen capture ELISA (Ag-ELISA) was able to detect more chronic infections. The specificity of the Ag-ELISA was found to be very good, but the sensitivity of the test should be improved. One way to detect more T. congolense and T. vivax infections was to lower the cut-off point of percent positivity from 10 to 5%. (author). 1 ref., 1 fig., 7 tabs.}
place = {IAEA}
year = {1997}
month = {Feb}
}
title = {The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe}
author = {Ries, R, and Nqindi, J}
abstractNote = {Blood and serum samples from cattle originating from tsetse free and tsetse infested areas were analyzed using the Buffy Coat technique and an ELISA to detect trypanosomes and trypanosomal antigens, respectively. The results of the two tests were compared and apparent sensitivity and trypanosomal prevalence were calculated. The BCT seemed to be the most suitable test to detect acute infections, while the antigen capture ELISA (Ag-ELISA) was able to detect more chronic infections. The specificity of the Ag-ELISA was found to be very good, but the sensitivity of the test should be improved. One way to detect more T. congolense and T. vivax infections was to lower the cut-off point of percent positivity from 10 to 5%. (author). 1 ref., 1 fig., 7 tabs.}
place = {IAEA}
year = {1997}
month = {Feb}
}