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The use of antigen-detection ELISA for the diagnosis of bovine trypanosomosis in Cote d`Ivoire

Abstract

An Antigen ELISA (Ag-ELISA) detecting circulating antigens of trypanosomes was evaluated in the central region of Cote d`Ivoire for the serodiagnosis of cattle trypanosomosis. Of 1423 sera examined, only 43 were positive in the MHCT/BCT, 105 (7%) were detected using stained blood smears, and 74 (5%) were found positive using the Ag-ELISA. The predominant trypanosome species was T. brucei, being present in 84% of the positive samples as detected by the BCT, in 96% using stained bloodsmears, and in 72% by Ag-ELISA. T.vivax was detected less frequently. The serological (ELISA) test did not detect all positive animals as found by the haematological techniques. However, the two techniques should be used in a complementary way to improve the diagnosis of the disease. The results confirm that the prevalence of trypanosomes in cattle is low in the study area. The low prevalence can be due to prophylaxis and therapy of livestock in combination with successful tsetse trapping. (author). 4 refs, 2 figs, 4 tabs.
Authors:
Kone, P; [1]  Komoin-Oka, C; N`Depo, A [2] 
  1. Laboratoire Regional de Pathologie Animale de Bouake (LANADA), Bouake (Cote d`Ivoire)
  2. Laboratoire Central de Pathologie Animale de Bingerville (LANADA), Bingerville (Cote d`Ivoire)
Publication Date:
Feb 01, 1997
Product Type:
Conference
Report Number:
IAEA-TECDOC-925; CONF-9504287-
Reference Number:
SCA: 553005; 553003; PA: AIX-28:036700; EDB-97:082359; SN: 97001795603
Resource Relation:
Conference: Workshop on epidemiological tools for monitoring trypanosomosis and tsetse control programmes, Addis Ababa (Ethiopia), 17-28 Apr 1995; Other Information: PBD: Feb 1997; Related Information: Is Part Of Application of an immunoassay method to improve the diagnosis and control of African trypanosomosis. Proceedings of the workshop; PB: 116 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DIAGNOSIS; ENZYME IMMUNOASSAY; TRYPANOSOMIASIS; ANTIGENS; BLOOD SERUM; COTE D`IVOIRE; GLOSSINA; MONITORING; TRYPANOSOMA
OSTI ID:
480984
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE97624611; TRN: XA9743589036700
Availability:
INIS; OSTI as DE97624611
Submitting Site:
INIS
Size:
pp. 31-37
Announcement Date:
Jun 20, 1997

Citation Formats

Kone, P, Komoin-Oka, C, and N`Depo, A. The use of antigen-detection ELISA for the diagnosis of bovine trypanosomosis in Cote d`Ivoire. IAEA: N. p., 1997. Web.
Kone, P, Komoin-Oka, C, & N`Depo, A. The use of antigen-detection ELISA for the diagnosis of bovine trypanosomosis in Cote d`Ivoire. IAEA.
Kone, P, Komoin-Oka, C, and N`Depo, A. 1997. "The use of antigen-detection ELISA for the diagnosis of bovine trypanosomosis in Cote d`Ivoire." IAEA.
@misc{etde_480984,
title = {The use of antigen-detection ELISA for the diagnosis of bovine trypanosomosis in Cote d`Ivoire}
author = {Kone, P, Komoin-Oka, C, and N`Depo, A}
abstractNote = {An Antigen ELISA (Ag-ELISA) detecting circulating antigens of trypanosomes was evaluated in the central region of Cote d`Ivoire for the serodiagnosis of cattle trypanosomosis. Of 1423 sera examined, only 43 were positive in the MHCT/BCT, 105 (7%) were detected using stained blood smears, and 74 (5%) were found positive using the Ag-ELISA. The predominant trypanosome species was T. brucei, being present in 84% of the positive samples as detected by the BCT, in 96% using stained bloodsmears, and in 72% by Ag-ELISA. T.vivax was detected less frequently. The serological (ELISA) test did not detect all positive animals as found by the haematological techniques. However, the two techniques should be used in a complementary way to improve the diagnosis of the disease. The results confirm that the prevalence of trypanosomes in cattle is low in the study area. The low prevalence can be due to prophylaxis and therapy of livestock in combination with successful tsetse trapping. (author). 4 refs, 2 figs, 4 tabs.}
place = {IAEA}
year = {1997}
month = {Feb}
}