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Trypanosomosis surveillance on Zanzibar island, using the trypanosomal antigen detection ELISA (enzyme-linked immunosorbent assay) technique

Abstract

The effectiveness of trypanosomosis control programs depends greatly on prior knowledge of basic data of the epidemiological situation of the disease, which in turns depends, among others, on the use of techniques that give a fairly quick and accurate diagnosis. An antigen-detection (Ag) ELISA was first introduced into Tanzania and validated at the Animal Disease Research Institute (ADRI) through the FAO/IAEA Research Contract (RC) No. 5030/NL. Incorporation of the Ag-ELISA technique into a FAO animal disease control project (1986-1993) on Unguja island, in 1992, revealed useful information of high trypanosomosis prevalence in an area previously declared free of the disease using just stained blood smears and buffy coat examinations. This triggered further efforts into more intensive surveys of the tsetse and trypanosomosis situation on Unguja island. The present study is a continuation of previous work in an effort to confirm the practical application of Ag-ELISA in trypanosomosis control operations. Results obtained from a known tsetse and trypanosomosis-free area, on Pemba island, showed a high specificity of the test for Trypanosoma congolense, T. vivax and T. brucei. A preliminary cut-off value of 10% (Percent Positivity = PP) was used. When the PP of 10 was used on sera of trypanosomosis-endemic areas  More>>
Authors:
Mbwambo, H A [1] 
  1. Animal Disease Research Inst. (ADRI), Dar-es-Salaam (Tanzania, United Republic of)
Publication Date:
Feb 01, 1997
Product Type:
Conference
Report Number:
IAEA-TECDOC-925; CONF-9504287-
Reference Number:
SCA: 553003; 553005; PA: AIX-28:036662; EDB-97:082332; SN: 97001795565
Resource Relation:
Conference: Workshop on epidemiological tools for monitoring trypanosomosis and tsetse control programmes, Addis Ababa (Ethiopia), 17-28 Apr 1995; Other Information: PBD: Feb 1997; Related Information: Is Part Of Application of an immunoassay method to improve the diagnosis and control of African trypanosomosis. Proceedings of the workshop; PB: 116 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DIAGNOSIS; ENZYME IMMUNOASSAY; TRYPANOSOMIASIS; ANTIGENS; BLOOD SERUM; CATTLE; GLOSSINA; MONITORING; STERILE INSECT RELEASE; STERILE MALE TECHNIQUE; TANZANIA; TRYPANOSOMA
OSTI ID:
480980
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE97624611; TRN: XA9743597036662
Availability:
INIS; OSTI as DE97624611
Submitting Site:
INIS
Size:
pp. 87-93
Announcement Date:

Citation Formats

Mbwambo, H A. Trypanosomosis surveillance on Zanzibar island, using the trypanosomal antigen detection ELISA (enzyme-linked immunosorbent assay) technique. IAEA: N. p., 1997. Web.
Mbwambo, H A. Trypanosomosis surveillance on Zanzibar island, using the trypanosomal antigen detection ELISA (enzyme-linked immunosorbent assay) technique. IAEA.
Mbwambo, H A. 1997. "Trypanosomosis surveillance on Zanzibar island, using the trypanosomal antigen detection ELISA (enzyme-linked immunosorbent assay) technique." IAEA.
@misc{etde_480980,
title = {Trypanosomosis surveillance on Zanzibar island, using the trypanosomal antigen detection ELISA (enzyme-linked immunosorbent assay) technique}
author = {Mbwambo, H A}
abstractNote = {The effectiveness of trypanosomosis control programs depends greatly on prior knowledge of basic data of the epidemiological situation of the disease, which in turns depends, among others, on the use of techniques that give a fairly quick and accurate diagnosis. An antigen-detection (Ag) ELISA was first introduced into Tanzania and validated at the Animal Disease Research Institute (ADRI) through the FAO/IAEA Research Contract (RC) No. 5030/NL. Incorporation of the Ag-ELISA technique into a FAO animal disease control project (1986-1993) on Unguja island, in 1992, revealed useful information of high trypanosomosis prevalence in an area previously declared free of the disease using just stained blood smears and buffy coat examinations. This triggered further efforts into more intensive surveys of the tsetse and trypanosomosis situation on Unguja island. The present study is a continuation of previous work in an effort to confirm the practical application of Ag-ELISA in trypanosomosis control operations. Results obtained from a known tsetse and trypanosomosis-free area, on Pemba island, showed a high specificity of the test for Trypanosoma congolense, T. vivax and T. brucei. A preliminary cut-off value of 10% (Percent Positivity = PP) was used. When the PP of 10 was used on sera of trypanosomosis-endemic areas (Mangapwani, Ndijani, Dunga and Kikungwi) on Unguja island, the results reflected the `real` trypanosomis situation in the affected area. This was most strongly felt in the Mangapwani area, where tsetse and trypanosomosis were considered under control by 1994 (no tsetse flies were caught and no samples were encountered positive by the buffy coat technique). However, it should be stressed that the buffy coat technique and the Ag-ELISA complement each other and should be used in conjunction. (author). 8 refs, 1 fig., 5 tabs.}
place = {IAEA}
year = {1997}
month = {Feb}
}