You need JavaScript to view this

Measurement of organic carbon quantity at chemoautorophic bacterium; Kagaku dokuritsu eiyo saikin ni okeru yuki tansoryo no sokutei ni tsuite

Abstract

Described herein is the method for analyzing quantity of organic carbon synthesized by chemoautotrophic bacterium. It is based on the combustion-infrared spectroscopy, which is normally adopted for quantitative analysis of organic carbon. The problems involved in the measurement of organic compounds synthesized by iron-oxidizing bacteria are noise by culture medium components, aging of gas analyzer and contamination with organic compounds from a silicon plug. The measures taken in this study against these problems include comparison of the results with a medium containing iron-oxidizing bacteria with those with a medium free of these bacteria, calibration with the standard solution for each measurement, and replacement of a silicone plug by a silicon cap. Organic carbon is measured by a TOC-5000 analyzer equipped with an automatic sample feeder ASI-5000. Biomass density is determined by the MPN method. It is confirmed that organic carbon quantity is almost in proportion to biomass density, a phenomenon which can be used to determine organic carbon quantity. 7 refs., 6 figs., 1 tab.
Authors:
Tsuda, I; Kato, K; Nozaki, K; [1]  Kurokawa, K [2] 
  1. Electrotechnical Laboratory, Tsukuba (Japan)
  2. Tokyo University of Agriculture and Technology, Tokyo (Japan)
Publication Date:
Oct 27, 1996
Product Type:
Conference
Report Number:
CONF-9610295-
Reference Number:
SCA: 440800; PA: NEDO-96:915520; EDB-97:075233; SN: 97001783064
Resource Relation:
Conference: JSES/JWEA joint conference (1996), 1996 nendo nihon taiyo energy gakkai nihon furyoku energy kyokai godo kenkyu happyokai, Yamagata (Japan), 31 Oct - 1 Nov 1996; Other Information: PBD: 27 Oct 1996; Related Information: Is Part Of Proceedings of JSES/JWEA Joint Conference (1996); PB: 406 p.; Taiyo/furyoku energy koen ronbunshu (1996)
Subject:
44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; MEASURING METHODS; INFRARED SPECTROMETERS; CARBON; BACTERIA; OXIDATION; IRON; CULTURE MEDIA; CONTAMINATION; CALIBRATION
OSTI ID:
472813
Research Organizations:
Japan Solar Energy Society, Tokyo (Japan)
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Other: ON: DE97744185; TRN: 96:915520
Availability:
Available from Japan Solar Energy Society, 44-14, Yoyogi 2-chome, Shibuya-ku, Tokyo, Japan; OSTI as DE97744185
Submitting Site:
NEDO
Size:
pp. 359-362
Announcement Date:

Citation Formats

Tsuda, I, Kato, K, Nozaki, K, and Kurokawa, K. Measurement of organic carbon quantity at chemoautorophic bacterium; Kagaku dokuritsu eiyo saikin ni okeru yuki tansoryo no sokutei ni tsuite. Japan: N. p., 1996. Web.
Tsuda, I, Kato, K, Nozaki, K, & Kurokawa, K. Measurement of organic carbon quantity at chemoautorophic bacterium; Kagaku dokuritsu eiyo saikin ni okeru yuki tansoryo no sokutei ni tsuite. Japan.
Tsuda, I, Kato, K, Nozaki, K, and Kurokawa, K. 1996. "Measurement of organic carbon quantity at chemoautorophic bacterium; Kagaku dokuritsu eiyo saikin ni okeru yuki tansoryo no sokutei ni tsuite." Japan.
@misc{etde_472813,
title = {Measurement of organic carbon quantity at chemoautorophic bacterium; Kagaku dokuritsu eiyo saikin ni okeru yuki tansoryo no sokutei ni tsuite}
author = {Tsuda, I, Kato, K, Nozaki, K, and Kurokawa, K}
abstractNote = {Described herein is the method for analyzing quantity of organic carbon synthesized by chemoautotrophic bacterium. It is based on the combustion-infrared spectroscopy, which is normally adopted for quantitative analysis of organic carbon. The problems involved in the measurement of organic compounds synthesized by iron-oxidizing bacteria are noise by culture medium components, aging of gas analyzer and contamination with organic compounds from a silicon plug. The measures taken in this study against these problems include comparison of the results with a medium containing iron-oxidizing bacteria with those with a medium free of these bacteria, calibration with the standard solution for each measurement, and replacement of a silicone plug by a silicon cap. Organic carbon is measured by a TOC-5000 analyzer equipped with an automatic sample feeder ASI-5000. Biomass density is determined by the MPN method. It is confirmed that organic carbon quantity is almost in proportion to biomass density, a phenomenon which can be used to determine organic carbon quantity. 7 refs., 6 figs., 1 tab.}
place = {Japan}
year = {1996}
month = {Oct}
}