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A modified direct insulin lodination for insulin radioreceptor assay in human erythrocytes

Abstract

A substitution of iodine in to the inulin molecule will easily lead to a decreased hormonal activity. Nevertheless it cannot be concluded without further investigation that one or several of the tyrosyl groups are directly involve in the degree of iodination and the loss activity has been demonstrated by many workers, (Frenkel-Conrat 1950, and de Zoeten and van Strik, 1961). On the basis of the Frenkel-Conrat experiments, lee 1957 suggested that a mono-substitution of iodine in two tyrosyl groups is possible without loss of biological activity. In general, iodination is easy to perform as usually carried out at room temperature at PH of 7.5 in a phosphate buffer medium (Megahed et al., 1976, 1979). Reaction between antigen and antibody in radioimmunoassay (RIA), immunoradiometric assay (IRMA) and radioreceptor assay (RRA), is detected by radiation emitted from a radioisotope incorporated into the antigen or antibody molecule. Ideally the radiolabelled molecule has an immunoreactivity identical to the natural molecule and this behaves in the same way as in the assay procedure. Oxidation of 125 I-iodide gives rise to the 125 I-iodination which in a mildly alkaline PH (7.5) reacts with the phenolic benzene ring of tyrosine and tyrosyl residues by a process of  More>>
Authors:
Elnabrawie, F S; Megahed, Y M; Fahim, F A; Ahmed, A M [1] 
  1. Middle Eastern Regional radioisotope center for Arab Countries biochemistry dept. Faculty of science Ain Shams university, cairo, (Egypt)
Publication Date:
Oct 01, 1995
Product Type:
Miscellaneous
Report Number:
INIS-EG-001; CONF-9411323-
Reference Number:
SCA: 550601; PA: AIX-28:030512; EDB-97:057684; SN: 97001765187
Resource Relation:
Conference: 2. Arab conference on the peaceful uses of atomic energy, Cairo (Egypt), 5-9 Nov 1994; Other Information: PBD: Oct 1995; Related Information: Is Part Of Proceedings of the second Arab conference on the peaceful uses of atomic energy. Part II: A and B; Barakat, M.F.; El-Mashri, S.M. [eds.]; PB: 1199 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ERYTHROCYTES; RADIORECEPTOR ASSAY; INSULIN; IODINATION; ANIMAL CELLS; EXPERIMENTAL DATA; IODINE 125; RADIOIMMUNOASSAY
OSTI ID:
456023
Research Organizations:
Atomic Energy Authority, Cairo (Egypt); Arab Atomic Energy Agency (AAEA), Tunis (Tunisia); Middle Eastern Regional Radioisotope Centre for the Arab Countries, Cairo (Egypt)
Country of Origin:
Egypt
Language:
English
Other Identifying Numbers:
Other: ON: DE97620040; ISBN 9973-9927-3-3; TRN: EG9601671030512
Availability:
INIS; OSTI as DE97620040
Submitting Site:
INIS
Size:
pp. 525
Announcement Date:

Citation Formats

Elnabrawie, F S, Megahed, Y M, Fahim, F A, and Ahmed, A M. A modified direct insulin lodination for insulin radioreceptor assay in human erythrocytes. Egypt: N. p., 1995. Web.
Elnabrawie, F S, Megahed, Y M, Fahim, F A, & Ahmed, A M. A modified direct insulin lodination for insulin radioreceptor assay in human erythrocytes. Egypt.
Elnabrawie, F S, Megahed, Y M, Fahim, F A, and Ahmed, A M. 1995. "A modified direct insulin lodination for insulin radioreceptor assay in human erythrocytes." Egypt.
@misc{etde_456023,
title = {A modified direct insulin lodination for insulin radioreceptor assay in human erythrocytes}
author = {Elnabrawie, F S, Megahed, Y M, Fahim, F A, and Ahmed, A M}
abstractNote = {A substitution of iodine in to the inulin molecule will easily lead to a decreased hormonal activity. Nevertheless it cannot be concluded without further investigation that one or several of the tyrosyl groups are directly involve in the degree of iodination and the loss activity has been demonstrated by many workers, (Frenkel-Conrat 1950, and de Zoeten and van Strik, 1961). On the basis of the Frenkel-Conrat experiments, lee 1957 suggested that a mono-substitution of iodine in two tyrosyl groups is possible without loss of biological activity. In general, iodination is easy to perform as usually carried out at room temperature at PH of 7.5 in a phosphate buffer medium (Megahed et al., 1976, 1979). Reaction between antigen and antibody in radioimmunoassay (RIA), immunoradiometric assay (IRMA) and radioreceptor assay (RRA), is detected by radiation emitted from a radioisotope incorporated into the antigen or antibody molecule. Ideally the radiolabelled molecule has an immunoreactivity identical to the natural molecule and this behaves in the same way as in the assay procedure. Oxidation of 125 I-iodide gives rise to the 125 I-iodination which in a mildly alkaline PH (7.5) reacts with the phenolic benzene ring of tyrosine and tyrosyl residues by a process of electrophilic attack.}
place = {Egypt}
year = {1995}
month = {Oct}
}