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Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity.

Abstract

The response to the extract of the egyptian wild herb Calendula Micrantha, with the Molluscicidal activity, was examined in the unicellular no bacterium Synechococcus sp. strain PCC 6301. growth and chlorophyll of the cells were only slightly affected by low plant extract concentrations but were drastically reduced by high concentration. the rate of protein synthesis progressively decreased by increasing extract concentration. the cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161 K (161.000), 96.7 K, 93.4 K, 69.9 K, 59 K, 49 K, 45 K, 35 K, 32.4 K, 28 K, 24 K, 21.7 K, 18 K and 16 K based on their mobilities in gel electrophoresis. these initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. some of induced polypeptides are similar to that known to occur in other stresses specially heat shock stress. 3 figs.
Authors:
Hammouda, O H.E.; [1]  Borbely, G [2] 
  1. Botany Department, Faculty of Science, Cairo University, Beni-Suef branch. Beni Suef (Egypt)
  2. Institute of plant physiology, Biological Research Center, Szeged H-6701, (Hungary)
Publication Date:
Oct 01, 1995
Product Type:
Miscellaneous
Report Number:
INIS-EG-001; CONF-9411323-
Reference Number:
SCA: 553002; PA: AIX-28:030253; EDB-97:057864; SN: 97001765072
Resource Relation:
Conference: 2. Arab conference on the peaceful uses of atomic energy, Cairo (Egypt), 5-9 Nov 1994; Other Information: PBD: Oct 1995; Related Information: Is Part Of Proceedings of the second Arab conference on the peaceful uses of atomic energy. Part II: A and B; Barakat, M.F.; El-Mashri, S.M. [eds.]; PB: 1199 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; UNICELLULAR ALGAE; LABELLING; PROTEINS; CHLOROPHYLL; EGYPTIAN ARAB REPUBLIC; EXPERIMENTAL DATA; METHIONINE; MOLLUSCS; POLYPEPTIDES
OSTI ID:
455986
Research Organizations:
Atomic Energy Authority, Cairo (Egypt); Arab Atomic Energy Agency (AAEA), Tunis (Tunisia); Middle Eastern Regional Radioisotope Centre for the Arab Countries, Cairo (Egypt)
Country of Origin:
Egypt
Language:
English
Other Identifying Numbers:
Other: ON: DE97620040; ISBN 9973-9927-3-3; TRN: EG9601726030253
Availability:
INIS; OSTI as DE97620040
Submitting Site:
INIS
Size:
pp. 1189
Announcement Date:
Jan 21, 2004

Citation Formats

Hammouda, O H.E., and Borbely, G. Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity.. Egypt: N. p., 1995. Web.
Hammouda, O H.E., & Borbely, G. Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity.. Egypt.
Hammouda, O H.E., and Borbely, G. 1995. "Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity." Egypt.
@misc{etde_455986,
title = {Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity.}
author = {Hammouda, O H.E., and Borbely, G}
abstractNote = {The response to the extract of the egyptian wild herb Calendula Micrantha, with the Molluscicidal activity, was examined in the unicellular no bacterium Synechococcus sp. strain PCC 6301. growth and chlorophyll of the cells were only slightly affected by low plant extract concentrations but were drastically reduced by high concentration. the rate of protein synthesis progressively decreased by increasing extract concentration. the cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161 K (161.000), 96.7 K, 93.4 K, 69.9 K, 59 K, 49 K, 45 K, 35 K, 32.4 K, 28 K, 24 K, 21.7 K, 18 K and 16 K based on their mobilities in gel electrophoresis. these initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. some of induced polypeptides are similar to that known to occur in other stresses specially heat shock stress. 3 figs.}
place = {Egypt}
year = {1995}
month = {Oct}
}