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A study of aluminium-exposed fish using a scanning proton microprobe

Abstract

A major problem has arisen in Europe with the depopulation of fresh water fish in lakes and streams collecting acid rain. The sensitivity to acidification is species specific and appears to be associated with metal levels. The Scanning Proton Microprobe (SPMP) at the Micro Analytical Research Centre of the University of Melbourne was used to study the subcellular distribution of aluminium and other elements in the gills of fish exposed to acidified water with elevated Al-levels. Experiments were performed on thin sections taken from fish exposed to media with different pH and aluminium concentration. Aluminium was found on the surface of the gill lamellae, but also inside the tissue. Bulk analysis of the gills showed much higher concentrations in the aluminium-exposed fish, compared to the control ones, but no information regarding the actual accumulation sites can be inferred. Extensive study of damage done to the sample by intense proton beams during elemental analysis was performed with scanning transmission ion microscopy. 3 refs., 3 figs.
Authors:
Cholewa, M; Legge, G L.F.; [1]  Eeckhaoudt, S; Van Grieken, R [2] 
  1. Melbourne Univ., Parkville, VIC (Australia). School of Physics
  2. Universitaire Instelling Antwerpen, Antwerp (Belgium)
Publication Date:
Dec 31, 1993
Product Type:
Miscellaneous
Report Number:
INIS-mf-15527; CONF-9311143-
Reference Number:
SCA: 400101; 553006; PA: AIX-28:024631; EDB-97:042264; SN: 97001752247
Resource Relation:
Conference: 8. Australian conference on nuclear techniques of analysis, Sydney (Australia), 17-19 Nov 1993; Other Information: PBD: 1993; Related Information: Is Part Of Proceedings of the 8. Australian conference on nuclear techniques of analysis; PB: 194 p.
Subject:
40 CHEMISTRY; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ALUMINIUM; PROTON MICROPROBE ANALYSIS; UPTAKE; FISHES; BIOLOGICAL AVAILABILITY; BIOLOGICAL MARKERS; EXPERIMENTAL DATA; QUANTITATIVE CHEMICAL ANALYSIS; WATER POLLUTION
OSTI ID:
445877
Country of Origin:
Australia
Language:
English
Other Identifying Numbers:
Other: ON: DE97616714; TRN: AU9715780024631
Availability:
INIS; OSTI as DE97616714
Submitting Site:
AUN
Size:
pp. 100-102
Announcement Date:
Mar 28, 1997

Citation Formats

Cholewa, M, Legge, G L.F., Eeckhaoudt, S, and Van Grieken, R. A study of aluminium-exposed fish using a scanning proton microprobe. Australia: N. p., 1993. Web.
Cholewa, M, Legge, G L.F., Eeckhaoudt, S, & Van Grieken, R. A study of aluminium-exposed fish using a scanning proton microprobe. Australia.
Cholewa, M, Legge, G L.F., Eeckhaoudt, S, and Van Grieken, R. 1993. "A study of aluminium-exposed fish using a scanning proton microprobe." Australia.
@misc{etde_445877,
title = {A study of aluminium-exposed fish using a scanning proton microprobe}
author = {Cholewa, M, Legge, G L.F., Eeckhaoudt, S, and Van Grieken, R}
abstractNote = {A major problem has arisen in Europe with the depopulation of fresh water fish in lakes and streams collecting acid rain. The sensitivity to acidification is species specific and appears to be associated with metal levels. The Scanning Proton Microprobe (SPMP) at the Micro Analytical Research Centre of the University of Melbourne was used to study the subcellular distribution of aluminium and other elements in the gills of fish exposed to acidified water with elevated Al-levels. Experiments were performed on thin sections taken from fish exposed to media with different pH and aluminium concentration. Aluminium was found on the surface of the gill lamellae, but also inside the tissue. Bulk analysis of the gills showed much higher concentrations in the aluminium-exposed fish, compared to the control ones, but no information regarding the actual accumulation sites can be inferred. Extensive study of damage done to the sample by intense proton beams during elemental analysis was performed with scanning transmission ion microscopy. 3 refs., 3 figs.}
place = {Australia}
year = {1993}
month = {Dec}
}