You need JavaScript to view this

Tissue culture of adult larch as a tool for breeding purposes

Abstract

Aimed at the identical reproduction of genotypes which are considered superior different methods were tested to establish and to propagate tissue cultures from old larch trees (L. decidua, L. kaempferi, L. sukaczewii, L. gmelinii, L. eurolepis). Serial subcultures without phytohormones (shoot tip propagation) led to the establishment of clone lines. After ten subcultures propagation velocity, shoot morphology and rooting behavior were similar to juvenile plant material. Serial subcultures which included a cytokinin induction led to the formation of adventitious shoot clusters (adventitious bud propagation). Adventitious shoots derived from male flowers of one L. kaempferi clone could be propagated via shoot tip propagation. Micrografting of meristems in vitro resulted in a regained rooting capacity of green cuttings from micrografts. Combining these in vitro techniques offers now the possibility to propagate selected mature larch trees for different breeding purposes. 23 refs, 5 figs, 2 tabs
Authors:
Ewald, D; Kretzschmar, U [1] 
  1. Federal Research Centre of Forestry and Forest Products, Waldsieversdorf (Germany). Inst. for Forest Tree Biology
Publication Date:
Dec 31, 1995
Product Type:
Conference
Report Number:
SLU-SSKTL-R-39; CONF-9507255-; CONF-9507256-
Reference Number:
SCA: 090700; PA: SWD-96:007673; EDB-96:169913; SN: 96001691685
Resource Relation:
Conference: Larch genetics and breeding. IUFRO Working Party S2.02-07, Remningstorp (Sweden); Siljansfors (Sweden), 31 Jul - 4 Aug 1995; 31 Jul - 4 Aug 1995; Other Information: PBD: 1995; Related Information: Is Part Of Proceedings. Larch genetics and breeding. Research findings and ecological-silvicultural demands; Martinsson, Owe [ed.]; PB: 214 p.
Subject:
09 BIOMASS FUELS; LARCHES; PLANT BREEDING; SILVICULTURE; TISSUE CULTURES; GRAFTS
OSTI ID:
396598
Research Organizations:
Swedish Univ. of Agricultural Sciences, Umeaa (Sweden). Dept. of Silviculture
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0348-8969; Other: ON: DE97711493; TRN: SE9607673
Availability:
OSTI as DE97711493
Submitting Site:
SWD
Size:
pp. 153-163
Announcement Date:

Citation Formats

Ewald, D, and Kretzschmar, U. Tissue culture of adult larch as a tool for breeding purposes. Sweden: N. p., 1995. Web.
Ewald, D, & Kretzschmar, U. Tissue culture of adult larch as a tool for breeding purposes. Sweden.
Ewald, D, and Kretzschmar, U. 1995. "Tissue culture of adult larch as a tool for breeding purposes." Sweden.
@misc{etde_396598,
title = {Tissue culture of adult larch as a tool for breeding purposes}
author = {Ewald, D, and Kretzschmar, U}
abstractNote = {Aimed at the identical reproduction of genotypes which are considered superior different methods were tested to establish and to propagate tissue cultures from old larch trees (L. decidua, L. kaempferi, L. sukaczewii, L. gmelinii, L. eurolepis). Serial subcultures without phytohormones (shoot tip propagation) led to the establishment of clone lines. After ten subcultures propagation velocity, shoot morphology and rooting behavior were similar to juvenile plant material. Serial subcultures which included a cytokinin induction led to the formation of adventitious shoot clusters (adventitious bud propagation). Adventitious shoots derived from male flowers of one L. kaempferi clone could be propagated via shoot tip propagation. Micrografting of meristems in vitro resulted in a regained rooting capacity of green cuttings from micrografts. Combining these in vitro techniques offers now the possibility to propagate selected mature larch trees for different breeding purposes. 23 refs, 5 figs, 2 tabs}
place = {Sweden}
year = {1995}
month = {Dec}
}