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Effects of maternal exposure to estrogen and PCB on different life stages of Zebrafish (Danio rerio)

Journal Article:

Abstract

PCBs have been found to impair both reproduction and development in fish. We have investigated the effects of 3 PCB congeners, 2,3,3`,4,4`,5,6-HpCB (PCB-190); 2,3,4,4`-TeCB (PCB-60); and 2,2`,4,6,6`-PeCB (PCB-104), and the estrogenic hormone 17{beta}-estradiol on fecundity, early life-stage mortality, gross morphology and histology of zebrafish (Danio rerio). While none of the studied substances reduced fecundity, they increased embryo and larval mortality. The most severe effects on viability were observed following treatment with 17{beta}-estradiol or the weakly estrogenic PCB-104. Following 17{beta}-estradiol or PCB-104 exposure, mortality continued through the yolksac absorption phase. PCB-60, on the other hand, resulted in mortality between the 30% epiboly stage and 75% epiboly stage. At the same time as embryos started to die, embryo development and hatching were delayed. PCB-190 showed only moderate effects on early-life stage mortality. The fish were reared until sexual maturation where after they were subjected to gross morphological and histological analyses. Changes in morphology were observed following PCB-104 and PCB-190 treatment. Both substances gave rise to craniofacial malformations while PCB-104 also led to lordosis in females and scoliosis in fish of both sexes. From histological analysis it was found that PCB-104 and 17{beta}-estradiol resulted in karyorrhexis and karyolysis in the kidney. Possible signs  More>>
Authors:
Olsson, Per-Erik; Westerlund, L; Billsson, K; Berg, A H; [1]  Teh, S J; Hinton, D E; [2]  Tysklind, M; [3]  Nilsson, Jan; Eriksson, Lars-Ove [4] 
  1. Umeaa Univ. (Sweden). Dept. of Cellular and Developmental Biology
  2. California Univ., Davis, CA (United States). Dept. of Anatomy, Physiology and Cell Biology
  3. Umeaa Univ., (Sweden). Dept. of Environmental Chemistry
  4. Swedish Univ. of Agricultural Sciences, Umeaa (Sweden). Dept. of Aquaculture
Publication Date:
Feb 01, 1999
Product Type:
Journal Article
Reference Number:
SCA: 560300; PA: SWD-99:007158; EDB-99:057145; SN: 99002075289
Resource Relation:
Journal Name: Ambio; Journal Volume: 28; Journal Issue: 1; Other Information: PBD: Feb 1999
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; BIOLOGICAL EFFECTS; POLYCHLORINATED BIPHENYLS; ESTROGENS; FISHES; MORTALITY; MORPHOLOGICAL CHANGES; REPRODUCTIVE DISORDERS
OSTI ID:
344548
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Journal ID: AMBOCX; ISSN 0044-7447; TRN: SE9907158
Submitting Site:
SWD
Size:
pp. 100-106
Announcement Date:

Journal Article:

Citation Formats

Olsson, Per-Erik, Westerlund, L, Billsson, K, Berg, A H, Teh, S J, Hinton, D E, Tysklind, M, Nilsson, Jan, and Eriksson, Lars-Ove. Effects of maternal exposure to estrogen and PCB on different life stages of Zebrafish (Danio rerio). Sweden: N. p., 1999. Web.
Olsson, Per-Erik, Westerlund, L, Billsson, K, Berg, A H, Teh, S J, Hinton, D E, Tysklind, M, Nilsson, Jan, & Eriksson, Lars-Ove. Effects of maternal exposure to estrogen and PCB on different life stages of Zebrafish (Danio rerio). Sweden.
Olsson, Per-Erik, Westerlund, L, Billsson, K, Berg, A H, Teh, S J, Hinton, D E, Tysklind, M, Nilsson, Jan, and Eriksson, Lars-Ove. 1999. "Effects of maternal exposure to estrogen and PCB on different life stages of Zebrafish (Danio rerio)." Sweden.
@misc{etde_344548,
title = {Effects of maternal exposure to estrogen and PCB on different life stages of Zebrafish (Danio rerio)}
author = {Olsson, Per-Erik, Westerlund, L, Billsson, K, Berg, A H, Teh, S J, Hinton, D E, Tysklind, M, Nilsson, Jan, and Eriksson, Lars-Ove}
abstractNote = {PCBs have been found to impair both reproduction and development in fish. We have investigated the effects of 3 PCB congeners, 2,3,3`,4,4`,5,6-HpCB (PCB-190); 2,3,4,4`-TeCB (PCB-60); and 2,2`,4,6,6`-PeCB (PCB-104), and the estrogenic hormone 17{beta}-estradiol on fecundity, early life-stage mortality, gross morphology and histology of zebrafish (Danio rerio). While none of the studied substances reduced fecundity, they increased embryo and larval mortality. The most severe effects on viability were observed following treatment with 17{beta}-estradiol or the weakly estrogenic PCB-104. Following 17{beta}-estradiol or PCB-104 exposure, mortality continued through the yolksac absorption phase. PCB-60, on the other hand, resulted in mortality between the 30% epiboly stage and 75% epiboly stage. At the same time as embryos started to die, embryo development and hatching were delayed. PCB-190 showed only moderate effects on early-life stage mortality. The fish were reared until sexual maturation where after they were subjected to gross morphological and histological analyses. Changes in morphology were observed following PCB-104 and PCB-190 treatment. Both substances gave rise to craniofacial malformations while PCB-104 also led to lordosis in females and scoliosis in fish of both sexes. From histological analysis it was found that PCB-104 and 17{beta}-estradiol resulted in karyorrhexis and karyolysis in the kidney. Possible signs of bile stasis were observed following 17{beta}-estradiol and PCB-190 treatment. Some effects were observed on the gonads, including areas in the ovary showing atresia and limited failure of testicular spermatogenesis in 17{beta}-estradiol, PCB-104, and PCB-60 treated fish. While all studied substances resulted in effects on offspring, the observation that estrogenic substances are highly embryotoxic, raises concern that endocrine disrupting substances may severely reduce fish populations in polluted areas}
journal = {Ambio}
issue = {1}
volume = {28}
journal type = {AC}
place = {Sweden}
year = {1999}
month = {Feb}
}