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Study of the effects of the irradiation on pancreatic cells `in vivo` and `in vitro`; Estudio de los efectos de la irradiacion de celulas pancreaticas `in vivo` e `in vitro`

Abstract

Full text: The bone marrow, gastrointestinal epithelium, gonads, lymphocytes and skin suffer the major damage after whole body irradiation. In rodents, dose ranging from 2 to 10 Gy produce death between 10 and 30 days post-irradiation, being the pancreas one of the most resistant organs to the ionizing radiation. In our laboratory we irradiated batches of adult Sprague-Dawley rats weighting between 360 and 420 g with a source of {sup 137}Cs 1.1 x 10{sup 16} Bq. Doses of 2, 5, 6, 7, 8, 10, 12 and 15 Gy using 8 animals per dose were assayed. The resultant {sup 30}LD{sub 50} was 7.14 Gy. Pancreas were removed immediately after spontaneous death or when surviving animals were sacrificed 60 days post-irradiation. Specimens of 3-5 mm were fixed in formol-buffer, slices of 3-4 {mu}m were stained with hematoxylin-eosin e and microscopically observed. At 2-5 Gy dose no histological damage was observed. At higher dose capillary congestion was observed in animals died on day 4-5{sup th} post irradiation. In the surviving rats, fluency of lymphocytes in the periphery of the Langerhans` islets was seen. The radio sensibility parameters D{sub 0} and N were characterized `in vitro` using the human cell line PANC-1, derived from  More>>
Authors:
Rivera, E; Cricco, G; Martin, G; Cocca, C; Bergoc, R M; [1]  Croci, M [2] 
  1. Buenos Aires Univ. (Argentina). Facultad de Farmacia y Bioquimica
  2. Fundacion Dr. Ernesto Crescenti, Buenos Aires (Argentina)
Publication Date:
Dec 31, 1998
Product Type:
Conference
Report Number:
INIS-AR-137; CONF-9809134-
Reference Number:
SCA: 560152; PA: AIX-30:023969; EDB-99:057091; SN: 99002097816
Resource Relation:
Conference: 6. Argentine congress on radiation protection and nuclear safety, 6. Congreso argentino de proteccion radiologica y seguridad nuclear, Buenos Aires (Argentina), 22-24 Sep 1998; Other Information: PBD: 1998; Related Information: Is Part Of Proceedings of the 6. Argentine congress on radiation protection and nuclear safety; PB: 75 p.; Actas del 6. congreso argentino de proteccion radiologica y seguridad nuclear
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; IN VITRO; IN VIVO; IONIZING RADIATIONS; IRRADIATION; PANCREAS; RADIATION EFFECTS; RATS
OSTI ID:
342923
Research Organizations:
Sociedad Argentina de Radioproteccion, Buenos Aires (Argentina)
Country of Origin:
Argentina
Language:
Spanish
Other Identifying Numbers:
Other: ON: DE99623524; TRN: AR9900018023969
Availability:
INIS; OSTI as DE99623524
Submitting Site:
INIS
Size:
pp. 47
Announcement Date:

Citation Formats

Rivera, E, Cricco, G, Martin, G, Cocca, C, Bergoc, R M, and Croci, M. Study of the effects of the irradiation on pancreatic cells `in vivo` and `in vitro`; Estudio de los efectos de la irradiacion de celulas pancreaticas `in vivo` e `in vitro`. Argentina: N. p., 1998. Web.
Rivera, E, Cricco, G, Martin, G, Cocca, C, Bergoc, R M, & Croci, M. Study of the effects of the irradiation on pancreatic cells `in vivo` and `in vitro`; Estudio de los efectos de la irradiacion de celulas pancreaticas `in vivo` e `in vitro`. Argentina.
Rivera, E, Cricco, G, Martin, G, Cocca, C, Bergoc, R M, and Croci, M. 1998. "Study of the effects of the irradiation on pancreatic cells `in vivo` and `in vitro`; Estudio de los efectos de la irradiacion de celulas pancreaticas `in vivo` e `in vitro`." Argentina.
@misc{etde_342923,
title = {Study of the effects of the irradiation on pancreatic cells `in vivo` and `in vitro`; Estudio de los efectos de la irradiacion de celulas pancreaticas `in vivo` e `in vitro`}
author = {Rivera, E, Cricco, G, Martin, G, Cocca, C, Bergoc, R M, and Croci, M}
abstractNote = {Full text: The bone marrow, gastrointestinal epithelium, gonads, lymphocytes and skin suffer the major damage after whole body irradiation. In rodents, dose ranging from 2 to 10 Gy produce death between 10 and 30 days post-irradiation, being the pancreas one of the most resistant organs to the ionizing radiation. In our laboratory we irradiated batches of adult Sprague-Dawley rats weighting between 360 and 420 g with a source of {sup 137}Cs 1.1 x 10{sup 16} Bq. Doses of 2, 5, 6, 7, 8, 10, 12 and 15 Gy using 8 animals per dose were assayed. The resultant {sup 30}LD{sub 50} was 7.14 Gy. Pancreas were removed immediately after spontaneous death or when surviving animals were sacrificed 60 days post-irradiation. Specimens of 3-5 mm were fixed in formol-buffer, slices of 3-4 {mu}m were stained with hematoxylin-eosin e and microscopically observed. At 2-5 Gy dose no histological damage was observed. At higher dose capillary congestion was observed in animals died on day 4-5{sup th} post irradiation. In the surviving rats, fluency of lymphocytes in the periphery of the Langerhans` islets was seen. The radio sensibility parameters D{sub 0} and N were characterized `in vitro` using the human cell line PANC-1, derived from a pancreatic carcinoma, that maintain the characteristic of ductal differentiated cells. Cells were cultured in Rmi 1640, 10% FCS at 37 degree C, 5% of CO{sub 2} atmosphere. Cell monolayers in stationary phase were irradiated using the same {sup 137}Cs source with doses ranging from 0.5 to 18 Gy. Immediately after cells were tripsined and a single cell suspension was seeded in fresh medium. Colonies formed by 50 or more cells were counted 10 days later. Results were: N=2 and D{sub 0}=0.75 {+-}0.12 Gy. The obtained results allowed to characterize the type of histological lesions at high dose and the radio sensibility of pancreatic cells PANC-1. (author) [Espanol] Texto completo: La medula osea, epitelio gastrointestinal, gonadas, linfocitos y piel sufren los mayores danos despues de la irradiacion de cuerpo entero. En roedores, dosis de 2 a 10 Gy, producen la muerte entre 10 y 30 dias post-irradiacion, siendo el pancreas uno de los organos mas tolerantes a la radiacion ionizante. En nuestro laboratorio hemos irradiado lotes de ratas Sprague-Dawley adultas, de entre 360 y 420 g de peso, con una fuente de cesio 137 de 1,1 x10{sup 16} Bq con 2, 5, 6, 7, 8, 10, 12 y 15 Gy, empleandose 8 animales por dosis. La {sup 30}DL{sub 50} resultante fue de 7,14 Gy. Todos los pancreas fueron removidos cuando los animales murieron o bien cuando los sobrevivientes fueron sacrificados a los 60 dias post-irradiacion. Especimenes de 3-5 mm fueron fijados en formol-buffer, cortes de 3-4 {mu}m fueron tenidos con hematoxilina-eosina y observados microscopicamente. A dosis de 2-5 Gy no se observaron danios histologicos. A dosis mas altas, en los animales muertos a los 4-5 dias post-irradiacion se evidencio congestion capilar y en los sobrevivientes, afluencia de linfocitos en la periferia de los islotes de Langerhans. Por otra parte, se caracterizaron `in vitro` los parametros de radiosensiblidad D{sub 0} y N de celulas malignas PANC-1 derivadas de pancreas humanos, que retienen las caracteristicas tipicas de celulas ductales diferenciadas; las celulas se mantuvieron en medio RPMI 1640 suplementado con suero fetal bovino al 10%, en estufa a 37 grados C, 5% de CO{sub 2} y humedad adecuada. Las celulas se dejaron crecer a 70-80% de confluencia y en esas condiciones se las irradio con la misma fuente de cesio 137 con: 0,5; 1; 1,5; 2; 2,5; 3; 5; 8; 10; 12; 15 y 18 Gy. Los resultados obtenidos fueron: N=2 y D{sub 0}=0,75 {+-}0,12 Gy. Los resultados obtenidos permitieron caracterizar el tipo de lesiones histologicas a altas dosis y la radiosensibilidad de las celulas PANC-1. (autor)}
place = {Argentina}
year = {1998}
month = {Dec}
}