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Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina

Abstract

An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to Babesia bovis was evaluated by using sera of 874 cattle carrying B. bovis antibodies, 700 sera of uninfected cattle, and 357 sera from calves from 16 herds subjected to different B. bovis inoculation rates. The seropositive/ seronegative cut-off point set as double the mean percent positivity of negative cattle sera (= 16%). The sensitivity of the ELISA (four trials) ranged from 97.1% to 100% and the specificity (three trials) varied from 92.0% to 97.0%. The agreement between ELISA and immunofluorescent antibody test was {>=} 90.0% in 18 of 23 evaluations and it ranged from 86.0% to 88.0% in the remainder. The correlation coefficient between percentage of sera positive to ELISA and IFA test in 16 herds was 0.9958 (P<0.001). The ELISA has the advantages of a high sensitivity, objectivity and capacity to test large number of samples in short period of time and could replace the IFA test specially for epidemiological studies. (author) 11 refs, 1 fig., 2 tabs
Authors:
Echaide, S; Echaide, I E; Mangold, A J; Lugaresi, C I; Guglielmone, A A; [1]  Gaido, A B [2] 
  1. Estacion Experimental Agropecuaria, Instituto Nacional de Tecnologia Agropecuaria, Rafaela, Santa Fe (Argentina)
  2. Estacion Experimental Agropecuaria Salta, Salta (Argentina)
Publication Date:
Nov 01, 1998
Product Type:
Conference
Report Number:
IAEA-TECDOC-1055; CONF-9406444-
Reference Number:
SCA: 553005; PA: AIX-29:067704; EDB-99:011370; SN: 99002051808
Resource Relation:
Conference: Final research co-ordination meeting on immunoassay methods for the diagnosis and epidemiology of animal diseases in Latin America, Guadeloupe (France), 13-17 Jun 1994; Other Information: DN: 11 refs, 1 fig., 2 tabs; PBD: Nov 1998; Related Information: Is Part Of Diagnosis and epidemiology of animal diseases in Latin America. Proceedings of the final research co-ordination meetings of FAO/IAEA/SIDA co-ordinated research projects; PB: 302 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ARGENTINA; BABESIDAE; BLOOD SERUM; CATTLE; DIAGNOSIS; DIAGNOSTIC TECHNIQUES; ENZYME IMMUNOASSAY; EPIDEMIOLOGY; PARASITIC DISEASES; SENSITIVITY; VETERINARY MEDICINE
OSTI ID:
298115
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE99611277; TRN: XA9848674067704
Availability:
INIS; OSTI as DE99611277
Submitting Site:
INIS
Size:
pp. 267-272
Announcement Date:

Citation Formats

Echaide, S, Echaide, I E, Mangold, A J, Lugaresi, C I, Guglielmone, A A, and Gaido, A B. Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina. IAEA: N. p., 1998. Web.
Echaide, S, Echaide, I E, Mangold, A J, Lugaresi, C I, Guglielmone, A A, & Gaido, A B. Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina. IAEA.
Echaide, S, Echaide, I E, Mangold, A J, Lugaresi, C I, Guglielmone, A A, and Gaido, A B. 1998. "Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina." IAEA.
@misc{etde_298115,
title = {Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina}
author = {Echaide, S, Echaide, I E, Mangold, A J, Lugaresi, C I, Guglielmone, A A, and Gaido, A B}
abstractNote = {An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to Babesia bovis was evaluated by using sera of 874 cattle carrying B. bovis antibodies, 700 sera of uninfected cattle, and 357 sera from calves from 16 herds subjected to different B. bovis inoculation rates. The seropositive/ seronegative cut-off point set as double the mean percent positivity of negative cattle sera (= 16%). The sensitivity of the ELISA (four trials) ranged from 97.1% to 100% and the specificity (three trials) varied from 92.0% to 97.0%. The agreement between ELISA and immunofluorescent antibody test was {>=} 90.0% in 18 of 23 evaluations and it ranged from 86.0% to 88.0% in the remainder. The correlation coefficient between percentage of sera positive to ELISA and IFA test in 16 herds was 0.9958 (P<0.001). The ELISA has the advantages of a high sensitivity, objectivity and capacity to test large number of samples in short period of time and could replace the IFA test specially for epidemiological studies. (author) 11 refs, 1 fig., 2 tabs}
place = {IAEA}
year = {1998}
month = {Nov}
}