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Pathogen detection by the polymerase chain reaction

Technical Report:

Abstract

In recent years, significant advances in the knowledge of DNA and its make up have led to the development of a powerful technique called polymerase chain reaction (PCR). Since the advent of PCR, laboratories around the globe have been exploiting this technology to bridge limitations or to overcome common problems encountered in molecular biology techniques. In addition, this technology has been employed successfully in diagnostic and basic scientific research and development. The true potentials of this technology is realized in early detection of pathogens and genetic abnormalities. In this paper two PCR protocols are described. The first is for detection of HIV-1 DNA in blood, the other for detection of rabies virus RNA in brain cells. 6 refs, 3 figs, 1 tab.
Authors:
Chitpatima, S T; Settachan, D; Pornsilpatip, J; Visawapoka, U; [1]  Dvorak, D R [2] 
  1. Pramongkutklao College of Medicine, Bangkok (Thailand). Molecular Biology Lab.
  2. Amersham International Ltd., Singapore (Singapore)
Publication Date:
May 01, 1994
Product Type:
Technical Report
Report Number:
IAEA-TECDOC-748
Reference Number:
SCA: 550601; 553003; PA: AIX-26:033151; EDB-95:059863; ERA-20:013895; SN: 95001365330
Resource Relation:
Other Information: PBD: May 1994; Related Information: Is Part Of Radionuclides in molecular technology for diagnosis of communicable diseases; PB: 131 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; AIDS VIRUS; DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; RABIES; ANIMAL CELLS; BRAIN; DNA; DNA HYBRIDIZATION; DNA REPLICATION; ELECTROPHORESIS; LABELLING; OLIGONUCLEOTIDES; PATHOGENS; PHOSPHORUS 32; RNA; TISSUE CULTURES; TISSUES
OSTI ID:
25286
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE95624121; TRN: XA9437529033151
Availability:
INIS; OSTI as DE95624121
Submitting Site:
INIS
Size:
pp. 41-65
Announcement Date:
Jan 15, 2004

Technical Report:

Citation Formats

Chitpatima, S T, Settachan, D, Pornsilpatip, J, Visawapoka, U, and Dvorak, D R. Pathogen detection by the polymerase chain reaction. IAEA: N. p., 1994. Web.
Chitpatima, S T, Settachan, D, Pornsilpatip, J, Visawapoka, U, & Dvorak, D R. Pathogen detection by the polymerase chain reaction. IAEA.
Chitpatima, S T, Settachan, D, Pornsilpatip, J, Visawapoka, U, and Dvorak, D R. 1994. "Pathogen detection by the polymerase chain reaction." IAEA.
@misc{etde_25286,
title = {Pathogen detection by the polymerase chain reaction}
author = {Chitpatima, S T, Settachan, D, Pornsilpatip, J, Visawapoka, U, and Dvorak, D R}
abstractNote = {In recent years, significant advances in the knowledge of DNA and its make up have led to the development of a powerful technique called polymerase chain reaction (PCR). Since the advent of PCR, laboratories around the globe have been exploiting this technology to bridge limitations or to overcome common problems encountered in molecular biology techniques. In addition, this technology has been employed successfully in diagnostic and basic scientific research and development. The true potentials of this technology is realized in early detection of pathogens and genetic abnormalities. In this paper two PCR protocols are described. The first is for detection of HIV-1 DNA in blood, the other for detection of rabies virus RNA in brain cells. 6 refs, 3 figs, 1 tab.}
place = {IAEA}
year = {1994}
month = {May}
}