Abstract
The use of synthetic non-coding RNAs for post-transcriptional regulation of gene expression has not only become a standard laboratory tool for gene functional studies but it has also opened up new perspectives in the design of new and potentially promising therapeutic strategies. Bioinformatics has provided researchers with a variety of tools for the design, the analysis, and the evaluation of RNAi agents such as small-interfering RNA (siRNA), short-hairpin RNA (shRNA), artificial microRNA (a-miR), and microRNA sponges. More recently, a new system for genome engineering based on the bacterial CRISPR-Cas9 system (Clustered Regularly Interspaced Short Palindromic Repeats), was shown to have the potential to also regulate gene expression at both transcriptional and post-transcriptional level in a more specific way. In this mini review, we present RNAi and CRISPRi design principles and discuss the advantages and limitations of the current design approaches.
Laganà, Alessandro;
[1]
Shasha, Dennis;
[2]
Croce, Carlo Maria
[1]
- Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH (United States)
- Courant Institute of Mathematical Sciences, New York University, New York, NY (United States)
Citation Formats
Laganà, Alessandro, Shasha, Dennis, and Croce, Carlo Maria.
Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools.
Switzerland: N. p.,
2014.
Web.
doi:10.3389/FBIOE.2014.00065.
Laganà, Alessandro, Shasha, Dennis, & Croce, Carlo Maria.
Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools.
Switzerland.
https://doi.org/10.3389/FBIOE.2014.00065
Laganà, Alessandro, Shasha, Dennis, and Croce, Carlo Maria.
2014.
"Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools."
Switzerland.
https://doi.org/10.3389/FBIOE.2014.00065.
@misc{etde_22688023,
title = {Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools}
author = {Laganà, Alessandro, Shasha, Dennis, and Croce, Carlo Maria}
abstractNote = {The use of synthetic non-coding RNAs for post-transcriptional regulation of gene expression has not only become a standard laboratory tool for gene functional studies but it has also opened up new perspectives in the design of new and potentially promising therapeutic strategies. Bioinformatics has provided researchers with a variety of tools for the design, the analysis, and the evaluation of RNAi agents such as small-interfering RNA (siRNA), short-hairpin RNA (shRNA), artificial microRNA (a-miR), and microRNA sponges. More recently, a new system for genome engineering based on the bacterial CRISPR-Cas9 system (Clustered Regularly Interspaced Short Palindromic Repeats), was shown to have the potential to also regulate gene expression at both transcriptional and post-transcriptional level in a more specific way. In this mini review, we present RNAi and CRISPRi design principles and discuss the advantages and limitations of the current design approaches.}
doi = {10.3389/FBIOE.2014.00065}
journal = []
volume = {2}
journal type = {AC}
place = {Switzerland}
year = {2014}
month = {Dec}
}
title = {Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools}
author = {Laganà, Alessandro, Shasha, Dennis, and Croce, Carlo Maria}
abstractNote = {The use of synthetic non-coding RNAs for post-transcriptional regulation of gene expression has not only become a standard laboratory tool for gene functional studies but it has also opened up new perspectives in the design of new and potentially promising therapeutic strategies. Bioinformatics has provided researchers with a variety of tools for the design, the analysis, and the evaluation of RNAi agents such as small-interfering RNA (siRNA), short-hairpin RNA (shRNA), artificial microRNA (a-miR), and microRNA sponges. More recently, a new system for genome engineering based on the bacterial CRISPR-Cas9 system (Clustered Regularly Interspaced Short Palindromic Repeats), was shown to have the potential to also regulate gene expression at both transcriptional and post-transcriptional level in a more specific way. In this mini review, we present RNAi and CRISPRi design principles and discuss the advantages and limitations of the current design approaches.}
doi = {10.3389/FBIOE.2014.00065}
journal = []
volume = {2}
journal type = {AC}
place = {Switzerland}
year = {2014}
month = {Dec}
}