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Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation

Abstract

In this study, Bacillus natto was irradiated by {sup 60}Co γ-ray, and activity was determined by Casein plate method in order to get high activity and thermostability strains. 60 strains with high activity were obtained through irradiation by 800 Gy {sup 60}Co γ-ray. In this dose, the positive mutation rate was 45%. Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃. (authors)
Authors:
Shuying, Li; Ying, Nie; Huan, Du; Xuanming, Tang; [1]  Zhonglin, Zhao; [2]  Xin, Ma; [3]  Yan, Li [4] 
  1. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing (China)
  2. College of Sciences, Henan Agricultural University, Zhengzhou (China)
  3. Agricultural Information Institute, Chinese Academic of Agricultural Sciences, Beijing (China)
  4. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou (China)
Publication Date:
Jun 15, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Nuclear Agricultural Sciences; Journal Volume: 27; Journal Issue: 6; Other Information: 4 figs., 11 refs.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BACILLUS; BACILLUS SUBTILIS; CASEIN; COBALT 60; GAMMA RADIATION; IRRADIATION; MUTATIONS; PHOSPHOTRANSFERASES; RADIATION DOSES; RADIOINDUCTION; SCREENING; THERMAL DEGRADATION
OSTI ID:
22600846
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 1000-8551; TRN: CN1700403045539
Submitting Site:
INIS
Size:
page(s) 782-785
Announcement Date:
Jun 13, 2017

Citation Formats

Shuying, Li, Ying, Nie, Huan, Du, Xuanming, Tang, Zhonglin, Zhao, Xin, Ma, and Yan, Li. Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation. China: N. p., 2013. Web.
Shuying, Li, Ying, Nie, Huan, Du, Xuanming, Tang, Zhonglin, Zhao, Xin, Ma, & Yan, Li. Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation. China.
Shuying, Li, Ying, Nie, Huan, Du, Xuanming, Tang, Zhonglin, Zhao, Xin, Ma, and Yan, Li. 2013. "Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation." China.
@misc{etde_22600846,
title = {Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation}
author = {Shuying, Li, Ying, Nie, Huan, Du, Xuanming, Tang, Zhonglin, Zhao, Xin, Ma, and Yan, Li}
abstractNote = {In this study, Bacillus natto was irradiated by {sup 60}Co γ-ray, and activity was determined by Casein plate method in order to get high activity and thermostability strains. 60 strains with high activity were obtained through irradiation by 800 Gy {sup 60}Co γ-ray. In this dose, the positive mutation rate was 45%. Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃. (authors)}
journal = []
issue = {6}
volume = {27}
journal type = {AC}
place = {China}
year = {2013}
month = {Jun}
}