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An approach to automated chromosome analysis; Etudes pour une methode d'automatisation des analyses chromosomiques

Thesis/Dissertation:

Abstract

The methods of approach developed with a view to automatic processing of the different stages of chromosome analysis are described in this study divided into three parts. Part 1 relates the study of automated selection of metaphase spreads, which operates a decision process in order to reject ail the non-pertinent images and keep the good ones. This approach has been achieved by Computing a simulation program that has allowed to establish the proper selection algorithms in order to design a kit of electronic logical units. Part 2 deals with the automatic processing of the morphological study of the chromosome complements in a metaphase: the metaphase photographs are processed by an optical-to-digital converter which extracts the image information and writes it out as a digital data set on a magnetic tape. For one metaphase image this data set includes some 200 000 grey values, encoded according to a 16, 32 or 64 grey-level scale, and is processed by a pattern recognition program isolating the chromosomes and investigating their characteristic features (arm tips, centromere areas), in order to get measurements equivalent to the lengths of the four arms. Part 3 studies a program of automated karyotyping by optimized pairing of human chromosomes.  More>>
Authors:
Publication Date:
May 03, 1972
Product Type:
Thesis/Dissertation
Report Number:
FRCEA-TH-6879
Resource Relation:
Other Information: 67 refs.; Available from the INIS Liaison Officer for France, see the 'INIS contacts' section of the INIS website for current contact and E-mail addresses: http://www.iaea.org/inis/Contacts/; These de Doctorat en Biologie Humaine
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ALGORITHMS; AUTOMATION; CENTROMERES; CHROMOSOMAL ABERRATIONS; CHROMOSOME SORTING; COMPUTERIZED SIMULATION; DATA ACQUISITION SYSTEMS; DATA ANALYSIS; DECISION MAKING; FORTRAN; FOURIER ANALYSIS; IMAGE PROCESSING; KARYOTYPE; MITOSIS; OPACITY; PATTERN RECOGNITION; PHOTOTRANSISTORS; R CODES; S CODES
OSTI ID:
22444009
Research Organizations:
Universite de PARIS VI, FACULTE DE MEDECINE PITIE-SALPETRIERE, U.E.R. 65 (France); Commissariat a l'Energie Atomique - CEA, Centre d'Etudes Nucleaires de Fontenay-aux-Roses, Departement de Protection, Section de Radiopathologie (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
TRN: FR1600101013550
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
360 page(s)
Announcement Date:
Mar 10, 2016

Thesis/Dissertation:

Citation Formats

Le Go, Roland. An approach to automated chromosome analysis; Etudes pour une methode d'automatisation des analyses chromosomiques. France: N. p., 1972. Web.
Le Go, Roland. An approach to automated chromosome analysis; Etudes pour une methode d'automatisation des analyses chromosomiques. France.
Le Go, Roland. 1972. "An approach to automated chromosome analysis; Etudes pour une methode d'automatisation des analyses chromosomiques." France.
@misc{etde_22444009,
title = {An approach to automated chromosome analysis; Etudes pour une methode d'automatisation des analyses chromosomiques}
author = {Le Go, Roland}
abstractNote = {The methods of approach developed with a view to automatic processing of the different stages of chromosome analysis are described in this study divided into three parts. Part 1 relates the study of automated selection of metaphase spreads, which operates a decision process in order to reject ail the non-pertinent images and keep the good ones. This approach has been achieved by Computing a simulation program that has allowed to establish the proper selection algorithms in order to design a kit of electronic logical units. Part 2 deals with the automatic processing of the morphological study of the chromosome complements in a metaphase: the metaphase photographs are processed by an optical-to-digital converter which extracts the image information and writes it out as a digital data set on a magnetic tape. For one metaphase image this data set includes some 200 000 grey values, encoded according to a 16, 32 or 64 grey-level scale, and is processed by a pattern recognition program isolating the chromosomes and investigating their characteristic features (arm tips, centromere areas), in order to get measurements equivalent to the lengths of the four arms. Part 3 studies a program of automated karyotyping by optimized pairing of human chromosomes. The data are derived from direct digitizing of the arm lengths by means of a BENSON digital reader. The program supplies' 1/ a list of the pairs, 2/ a graphic representation of the pairs so constituted according to their respective lengths and centromeric indexes, and 3/ another BENSON graphic drawing according to the author's own representation of the chromosomes, i.e. crosses with orthogonal arms, each branch being the accurate measurement of the corresponding chromosome arm. This conventionalized karyotype indicates on the last line the really abnormal or non-standard images unpaired by the program, which are of special interest for the biologist. (author) [French] Ce travail expose les methodes d'approche etudiees en vue d'une automatisation des differentes etapes de l'analyse chromosomique. Il est divise en trois tomes dont la matiere est decrite ci-apres. Dans le tome 1 est decrite l'etude d'un selecteur automatique de metaphases. Cet appareil doit realiser au cours du scanning d'une preparation, un processus de decision aboutissant a rejeter toutes images ininteressantes et a conserver les images pertinentes (bonnes metaphases). Ce probleme a ete aborde au moyen d'une etude sur ordinateur par un programme de simulation. La realisation de ce programme a permis d'etablir des algorithmes de selection dont la replique pourra etre realisee dans des circuits logiques electroniques. Dans le tome 2 est traite le probleme de l'automatisation de l'etude morphologique des chromosomes presents dans une metaphase. Cette automatisation necessite le traitement des photographies de metaphases par un appareil convertisseur optique-numerique qui extrait l'information-image et la transcrit sous la forme d'un fichier numerique. Ce fichier comporte pour une image de metaphase, environ 200 000 valeurs de gris, codees selon une echelle a 16, 32 ou 64 ni veaux. Ce fichier numerique est traite par un programme de reconnaissance de forme, qui isole les chromosomes et en recherche les traits caracteristiques: sommets de bras et region centromerique, dans le but d'obtenir des mensurations equivalentes aux longueurs des quatre bras. Le tome 3 etudie un programme d'etablissement automatique du caryotype humain, par appariement optimise des chromosomes. Les donnees proviennent de la numerisation directe des longueurs de bras, effectuee au moyen d'un lecteur digital BENSON. Le programme etablit une liste des appariements, un document graphique imprime representant les paires constituees, dans l'espace: longueur - index centromerique; enfin un document graphique obtenu sur traceur BENSON dans lequel l'auteur donne une representation personnelle des chromosomes, sous forme de croix a bras orthogonaux, dont chaque branche est la mesure exacte du bras de chromosome correspondant. Ce caryotype stylise comporte sur sa derniere ligne, les images anormales ou simplement non standard, rencontrees au cours du processus d'appariement et que le programme a laissees non classees. C'est sur ces images que doit donc se porter plus precisement l'attention du biologiste. (auteur)}
place = {France}
year = {1972}
month = {May}
}