You need JavaScript to view this

Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant

Abstract

Hydroponic experiments were conducted with seedlings of barley (Hordeum vulgare L.), comparing the wild type and an NR-deficient mutant (AZ 12/70), to monitor the NO{sub 3}{sup -} influx/efflux relations and the response to tungstate treatment using {sup 13}N-labelling. Upon treatment with the arginyl-residue-binding inhibitor phenylglyoxal both genotypes responded with an immediate depression of NO{sub 3}{sup -} influx. At low external NO{sub 3}{sup -} concentrations efflux was small (5-15% of the influx) and did not differ substantially between the two genotypes. Although the mutant was distinguished from the wild type by a thinner root system and a reduced shoot length, the total N distribution between roots and shoots and the N concentrations in the root and shoot tissues were fairly comparable. Substantially higher extractable NO{sub 3}{sup -} concentration in the shoots (but not roots) of the mutant may indicate that the capacity to reduce NO{sub 3}{sup -} was restricted due to the very low but still detectable NR activity in the root and shoot tissue. Nevertheless, the mutant must have had supplementary means of assimilating considerable amounts of NO{sub 3}{sup -} over the experimental growth period. At the induced stage, both barley genotypes responded to tungstate treatments with a comparable but  More>>
Authors:
Publication Date:
Jul 01, 1994
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Plant Nutrition; Journal Volume: 17; Journal Issue: 1; Other Information: FAO/AGRIS record; ARN: US19950035313; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ABUNDANCE; ANIMAL TISSUES; BARLEY; COMPARATIVE EVALUATIONS; CONCENTRATION RATIO; ECOLOGICAL CONCENTRATION; GENOTYPE; METABOLISM; MOLYBDENUM; MUTANTS; NITRATES; NITROGEN; NITROGEN 13; NUTRIENTS; PHYSIOLOGY; PLANT TISSUES; RESIDUES; ROOTS; SEEDLINGS; TUNGSTATES; TUNGSTEN
OSTI ID:
22443732
Research Organizations:
Norges Landbrukshoegskole, Ås (Norway)
Country of Origin:
FAO
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0190-4167; TRN: XF15A4788013273
Submitting Site:
INIS
Size:
page(s) 127-146
Announcement Date:
Mar 10, 2016

Citation Formats

Wieneke, J. Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant. FAO: N. p., 1994. Web. doi:10.1080/01904169409364714.
Wieneke, J. Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant. FAO. doi:10.1080/01904169409364714.
Wieneke, J. 1994. "Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant." FAO. doi:10.1080/01904169409364714. https://www.osti.gov/servlets/purl/10.1080/01904169409364714.
@misc{etde_22443732,
title = {Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant}
author = {Wieneke, J.}
abstractNote = {Hydroponic experiments were conducted with seedlings of barley (Hordeum vulgare L.), comparing the wild type and an NR-deficient mutant (AZ 12/70), to monitor the NO{sub 3}{sup -} influx/efflux relations and the response to tungstate treatment using {sup 13}N-labelling. Upon treatment with the arginyl-residue-binding inhibitor phenylglyoxal both genotypes responded with an immediate depression of NO{sub 3}{sup -} influx. At low external NO{sub 3}{sup -} concentrations efflux was small (5-15% of the influx) and did not differ substantially between the two genotypes. Although the mutant was distinguished from the wild type by a thinner root system and a reduced shoot length, the total N distribution between roots and shoots and the N concentrations in the root and shoot tissues were fairly comparable. Substantially higher extractable NO{sub 3}{sup -} concentration in the shoots (but not roots) of the mutant may indicate that the capacity to reduce NO{sub 3}{sup -} was restricted due to the very low but still detectable NR activity in the root and shoot tissue. Nevertheless, the mutant must have had supplementary means of assimilating considerable amounts of NO{sub 3}{sup -} over the experimental growth period. At the induced stage, both barley genotypes responded to tungstate treatments with a comparable but not complete depression of the NO{sub 3}{sup -} influx. Part of the NO{sub 3}{sup -} influx appears to be independent of the function of NR since an acceleration of the NO{sub 3}{sup -} uptake capacity to almost half the level of the controls occurred in both cultivars upon induction in spite of pretreatment with 150 micromolar WO{sub 4}{sup 2-} in a molybdenum-free solution prior to NO{sub 3}{sup -} induction. However, 600 micromolar tungstate treatment during the induction phase reduced NO{sub 3}{sup -} influx further (below 20%), but the plants of both cultivars were still able to recover almost completely. (author)}
doi = {10.1080/01904169409364714}
journal = {Journal of Plant Nutrition}
issue = {1}
volume = {17}
journal type = {AC}
place = {FAO}
year = {1994}
month = {Jul}
}