Abstract
Andrographolide, a bioactive diterpenoid, is identified in Andrographis paniculata. In this study, we investigated the pharmacokinetics and bioavailability of andrographolide in rats and studied whether andrographolide enhances antioxidant defense in a variety of tissues and protects against carbon tetrachloride-induced oxidative damage. After a single 50-mg/kg administration, the maximum plasma concentration of andrographolide was 1 μM which peaked at 30 min. The bioavailability of andrographolide was 1.19%. In a hepatoprotection study, rats were intragastrically dosed with 30 or 50 mg/kg andrographolide for 5 consecutive days. The results showed that andrographolide up-regulated glutamate cysteine ligase (GCL) catalytic and modifier subunits, superoxide dismutase (SOD)-1, heme oxygenase (HO)-1, and glutathione (GSH) S-transferase (GST) Ya/Yb protein and mRNA expression in the liver, heart, and kidneys. The activity of SOD, GST, and GSH reductase was also increased in rats dosed with andrographolide (p < 0.05). Immunoblot analysis and EMSA revealed that andrographolide increased nuclear Nrf2 contents and Nrf2 binding to DNA, respectively. After the 5-day andrographolide treatment, one group of animals was intraperitoneally injected with carbon tetrachloride (CCl{sub 4}) at day 6. Andrographolide pretreatment suppressed CCl{sub 4}-induced plasma aminotransferase activity and hepatic lipid peroxidation (p < 0.05). These results suggest that andrographolide is quickly absorbed in
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Chen, Haw-Wen;
[1]
Huang, Chin-Shiu;
[2]
Li, Chien-Chun;
[3]
Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)];
Lin, Ai-Hsuan;
Huang, Yu-Ju;
[1]
Wang, Tsu-Shing;
[4]
Yao, Hsien-Tsung;
[1]
Lii, Chong-Kuei;
[1]
Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)]
- Department of Nutrition, China Medical University, Taichung, Taiwan (China)
- Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)
- School of Nutrition, Chung Shan Medical University, Taichung, Taiwan (China)
- Department of Biomedical Science, Chung Shan Medical University, Taichung, Taiwan (China)
Citation Formats
Chen, Haw-Wen, Huang, Chin-Shiu, Li, Chien-Chun, Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)], Lin, Ai-Hsuan, Huang, Yu-Ju, Wang, Tsu-Shing, Yao, Hsien-Tsung, Lii, Chong-Kuei, and Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)].
Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats.
United States: N. p.,
2014.
Web.
doi:10.1016/J.TAAP.2014.07.024.
Chen, Haw-Wen, Huang, Chin-Shiu, Li, Chien-Chun, Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)], Lin, Ai-Hsuan, Huang, Yu-Ju, Wang, Tsu-Shing, Yao, Hsien-Tsung, Lii, Chong-Kuei, & Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)].
Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats.
United States.
https://doi.org/10.1016/J.TAAP.2014.07.024
Chen, Haw-Wen, Huang, Chin-Shiu, Li, Chien-Chun, Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)], Lin, Ai-Hsuan, Huang, Yu-Ju, Wang, Tsu-Shing, Yao, Hsien-Tsung, Lii, Chong-Kuei, and Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)].
2014.
"Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats."
United States.
https://doi.org/10.1016/J.TAAP.2014.07.024.
@misc{etde_22439846,
title = {Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats}
author = {Chen, Haw-Wen, Huang, Chin-Shiu, Li, Chien-Chun, Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)], Lin, Ai-Hsuan, Huang, Yu-Ju, Wang, Tsu-Shing, Yao, Hsien-Tsung, Lii, Chong-Kuei, and Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)]}
abstractNote = {Andrographolide, a bioactive diterpenoid, is identified in Andrographis paniculata. In this study, we investigated the pharmacokinetics and bioavailability of andrographolide in rats and studied whether andrographolide enhances antioxidant defense in a variety of tissues and protects against carbon tetrachloride-induced oxidative damage. After a single 50-mg/kg administration, the maximum plasma concentration of andrographolide was 1 μM which peaked at 30 min. The bioavailability of andrographolide was 1.19%. In a hepatoprotection study, rats were intragastrically dosed with 30 or 50 mg/kg andrographolide for 5 consecutive days. The results showed that andrographolide up-regulated glutamate cysteine ligase (GCL) catalytic and modifier subunits, superoxide dismutase (SOD)-1, heme oxygenase (HO)-1, and glutathione (GSH) S-transferase (GST) Ya/Yb protein and mRNA expression in the liver, heart, and kidneys. The activity of SOD, GST, and GSH reductase was also increased in rats dosed with andrographolide (p < 0.05). Immunoblot analysis and EMSA revealed that andrographolide increased nuclear Nrf2 contents and Nrf2 binding to DNA, respectively. After the 5-day andrographolide treatment, one group of animals was intraperitoneally injected with carbon tetrachloride (CCl{sub 4}) at day 6. Andrographolide pretreatment suppressed CCl{sub 4}-induced plasma aminotransferase activity and hepatic lipid peroxidation (p < 0.05). These results suggest that andrographolide is quickly absorbed in the intestinal tract in rats with a bioavailability of 1.19%. Andrographolide protects against chemical-induced oxidative damage by up-regulating the gene transcription and activity of antioxidant enzymes in various tissues. - Highlights: • The bioavailability of andrographolide is 1.19% in rats. • Plasma concentration reaches 1 μM after giving 50 mg/kg andrographolide. • Andrographolide up-regulates Nrf2-dependent antioxidant genes. • Andrographolide increases antioxidant defense in various tissues. • Andrographolide ameliorates carbon tetrachloride-induced hepatotoxicity in rats.}
doi = {10.1016/J.TAAP.2014.07.024}
journal = []
issue = {1}
volume = {280}
journal type = {AC}
place = {United States}
year = {2014}
month = {Oct}
}
title = {Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats}
author = {Chen, Haw-Wen, Huang, Chin-Shiu, Li, Chien-Chun, Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan (China)], Lin, Ai-Hsuan, Huang, Yu-Ju, Wang, Tsu-Shing, Yao, Hsien-Tsung, Lii, Chong-Kuei, and Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China)]}
abstractNote = {Andrographolide, a bioactive diterpenoid, is identified in Andrographis paniculata. In this study, we investigated the pharmacokinetics and bioavailability of andrographolide in rats and studied whether andrographolide enhances antioxidant defense in a variety of tissues and protects against carbon tetrachloride-induced oxidative damage. After a single 50-mg/kg administration, the maximum plasma concentration of andrographolide was 1 μM which peaked at 30 min. The bioavailability of andrographolide was 1.19%. In a hepatoprotection study, rats were intragastrically dosed with 30 or 50 mg/kg andrographolide for 5 consecutive days. The results showed that andrographolide up-regulated glutamate cysteine ligase (GCL) catalytic and modifier subunits, superoxide dismutase (SOD)-1, heme oxygenase (HO)-1, and glutathione (GSH) S-transferase (GST) Ya/Yb protein and mRNA expression in the liver, heart, and kidneys. The activity of SOD, GST, and GSH reductase was also increased in rats dosed with andrographolide (p < 0.05). Immunoblot analysis and EMSA revealed that andrographolide increased nuclear Nrf2 contents and Nrf2 binding to DNA, respectively. After the 5-day andrographolide treatment, one group of animals was intraperitoneally injected with carbon tetrachloride (CCl{sub 4}) at day 6. Andrographolide pretreatment suppressed CCl{sub 4}-induced plasma aminotransferase activity and hepatic lipid peroxidation (p < 0.05). These results suggest that andrographolide is quickly absorbed in the intestinal tract in rats with a bioavailability of 1.19%. Andrographolide protects against chemical-induced oxidative damage by up-regulating the gene transcription and activity of antioxidant enzymes in various tissues. - Highlights: • The bioavailability of andrographolide is 1.19% in rats. • Plasma concentration reaches 1 μM after giving 50 mg/kg andrographolide. • Andrographolide up-regulates Nrf2-dependent antioxidant genes. • Andrographolide increases antioxidant defense in various tissues. • Andrographolide ameliorates carbon tetrachloride-induced hepatotoxicity in rats.}
doi = {10.1016/J.TAAP.2014.07.024}
journal = []
issue = {1}
volume = {280}
journal type = {AC}
place = {United States}
year = {2014}
month = {Oct}
}