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Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines

Abstract

Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also determined. Results showed that GalN/LPS induced marked elevations in serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Edaravone significantly inhibited elevation of serum AST and ALT, accompanied by an improvement in histological findings. Edaravone lowered the levels of TNF-α and IL-6 and reduced the number of TUNEL-positive cells. In addition, 24 h after edaravone treatment, caspase-3 activity and mortality were reduced. Edaravone may effectively ameliorate GalN/LPS-induced liver injury in mice by reducing proinflammatory cytokines and inhibiting apoptosis.
Authors:
Zong, L.; [1]  No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)]; Yu, Q. H.; [2]  Du, Y. X.; [3]  Deng, X. M. [1] 
  1. Second Military Medical University, Changhai Hospital, Department of Anesthesiology, Shanghai, China, Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai (China)
  2. Second Military Medical University, Changhai Hospital, Department of Gastroenterology, Shanghai, China, Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai (China)
  3. No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)
Publication Date:
Mar 03, 2014
Product Type:
Journal Article
Resource Relation:
Journal Name: Brazilian Journal of Medical and Biological Research; Journal Volume: 47; Journal Issue: 3; Other Information: PMCID: PMC3982944; PMID: 24554039; OAI: oai:pubmedcentral.nih.gov:3982944; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; ENDOTOXINS; ENZYME IMMUNOASSAY; INFLAMMATION; INJECTION; INJURIES; LEVELS; LIVER; LYMPHOKINES; MICE
OSTI ID:
22437070
Country of Origin:
Brazil
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0100-879X; TRN: BR15$0338006604
Availability:
Available from http://dx.doi.org/10.1590/1414-431X20133186; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982944
Submitting Site:
BRN
Size:
page(s) 231-236
Announcement Date:
Mar 07, 2016

Citation Formats

Zong, L., No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)], Yu, Q. H., Du, Y. X., and Deng, X. M. Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines. Brazil: N. p., 2014. Web. doi:10.1590/1414-431X20133186.
Zong, L., No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)], Yu, Q. H., Du, Y. X., & Deng, X. M. Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines. Brazil. doi:10.1590/1414-431X20133186.
Zong, L., No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)], Yu, Q. H., Du, Y. X., and Deng, X. M. 2014. "Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines." Brazil. doi:10.1590/1414-431X20133186. https://www.osti.gov/servlets/purl/10.1590/1414-431X20133186.
@misc{etde_22437070,
title = {Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines}
author = {Zong, L., No. 82 Hospital of People's Liberation Army, Department of Anesthesiology, Jiangsu, China, Department of Anesthesiology, No. 82 Hospital of People's Liberation Army, Jiangsu (China)], Yu, Q. H., Du, Y. X., and Deng, X. M.}
abstractNote = {Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also determined. Results showed that GalN/LPS induced marked elevations in serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Edaravone significantly inhibited elevation of serum AST and ALT, accompanied by an improvement in histological findings. Edaravone lowered the levels of TNF-α and IL-6 and reduced the number of TUNEL-positive cells. In addition, 24 h after edaravone treatment, caspase-3 activity and mortality were reduced. Edaravone may effectively ameliorate GalN/LPS-induced liver injury in mice by reducing proinflammatory cytokines and inhibiting apoptosis.}
doi = {10.1590/1414-431X20133186}
journal = {Brazilian Journal of Medical and Biological Research}
issue = {3}
volume = {47}
journal type = {AC}
place = {Brazil}
year = {2014}
month = {Mar}
}