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Sterilization of Normal Human Plasma and Some of its Fractions by Means of Gamma Rays; Esterilizacion del plasma normal humano y algunas de sus fracciones por medio de radiaciones gamma

Conference:

Abstract

Owing to the frequency with which normal human plasma transmits hepatitis, various methods of sterilization have been tried. The method most used, but which has been shown to be ineffective, is sterilization of the liquid plasma with ultraviolet rays. The other method is the use of ß-propiolactone, but this has also been discontinued because of the changes it produces in the structure of plasma proteins. The object of the present study, which should be regarded as a preliminary report, is to present the results obtained by sterilizing, by means of gamma rays ({sup 60}Co, 1.3316 MeV) at doses of 2, 2. 5 and 3 Mrad, a substance which, like plasma, contains highly labile proteins easily able to undergo structural changes under irradiation. Pure fibrinogen, pure gamma globulin, and albumin of human origin were subjected to the same doses; the results were very satisfactory, in that no appreciable change could be demonstrated as regards the structure, solubility or chemical characteristics of the substances concerned. It was shown by means of simple coagulation tests that some of the proteins involved in this mechanism, such as prothrombin, die Power-Stuart factor and the Hageman factor, were practically unchanged. All the plasma samples and the  More>>
Authors:
López Martínez de Alva, L.; Crespo, Y M. [1] 
  1. Cortina Comision Nacional de Energia Nuclear (Mexico)
Publication Date:
Sep 15, 1967
Product Type:
Conference
Report Number:
IAEA-SM-92/37
Resource Relation:
Conference: Symposium on Radiosterilization of Medical Products, Budapest (Hungary), 5-9 Jun 1967, Panel Meeting on a Code of Practice for the Radiosterilization of Medical Products, Vienna (Austria), 5-9 Dec 1966; Other Information: 6 refs., 7 figs., 2 tabs.; Related Information: In: Radiosterilization of Medical Products and Recommended Code of Practice. Proceedings of a Symposium| 481 p.
Subject:
60 APPLIED LIFE SCIENCES; ALBUMINS; COBALT 60; FIBRINOGEN; GAMMA RADIATION; HEPATITIS; HUMAN POPULATIONS; LIQUIDS; MEV RANGE 01-10; PACKAGING; PROTHROMBIN; RADIATION DOSES; RADIOSTERILIZATION; SOLUBILITY; ULTRAVIOLET RADIATION; VIRUSES
OSTI ID:
22423970
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
Spanish
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA15M0436129897
Submitting Site:
INIS
Size:
page(s) 125-135
Announcement Date:
Jan 11, 2016

Conference:

Citation Formats

López Martínez de Alva, L., and Crespo, Y M. Sterilization of Normal Human Plasma and Some of its Fractions by Means of Gamma Rays; Esterilizacion del plasma normal humano y algunas de sus fracciones por medio de radiaciones gamma. IAEA: N. p., 1967. Web.
López Martínez de Alva, L., & Crespo, Y M. Sterilization of Normal Human Plasma and Some of its Fractions by Means of Gamma Rays; Esterilizacion del plasma normal humano y algunas de sus fracciones por medio de radiaciones gamma. IAEA.
López Martínez de Alva, L., and Crespo, Y M. 1967. "Sterilization of Normal Human Plasma and Some of its Fractions by Means of Gamma Rays; Esterilizacion del plasma normal humano y algunas de sus fracciones por medio de radiaciones gamma." IAEA.
@misc{etde_22423970,
title = {Sterilization of Normal Human Plasma and Some of its Fractions by Means of Gamma Rays; Esterilizacion del plasma normal humano y algunas de sus fracciones por medio de radiaciones gamma}
author = {López Martínez de Alva, L., and Crespo, Y M.}
abstractNote = {Owing to the frequency with which normal human plasma transmits hepatitis, various methods of sterilization have been tried. The method most used, but which has been shown to be ineffective, is sterilization of the liquid plasma with ultraviolet rays. The other method is the use of ß-propiolactone, but this has also been discontinued because of the changes it produces in the structure of plasma proteins. The object of the present study, which should be regarded as a preliminary report, is to present the results obtained by sterilizing, by means of gamma rays ({sup 60}Co, 1.3316 MeV) at doses of 2, 2. 5 and 3 Mrad, a substance which, like plasma, contains highly labile proteins easily able to undergo structural changes under irradiation. Pure fibrinogen, pure gamma globulin, and albumin of human origin were subjected to the same doses; the results were very satisfactory, in that no appreciable change could be demonstrated as regards the structure, solubility or chemical characteristics of the substances concerned. It was shown by means of simple coagulation tests that some of the proteins involved in this mechanism, such as prothrombin, die Power-Stuart factor and the Hageman factor, were practically unchanged. All the plasma samples and the various proteins were previously lyophilized to give a maximum moisture content of 0.034%, in order to avoid ionizing the water content into oxygenated water, which would modify and oxidize the proteins. It was shown that lyophilized plasmas initially contaminated with different strains and viruses remained sterile with doses as low as 2 Mrad. Finally, it was shown that this method is simple and practical, since sterilization can be checked in the final packaging. (author) [Spanish] Debido a la frecuencia con que el plasma normal humano transmitia la hepatitis, se han intentado diferentes métodos de esterilizacion. El método más usado pero que ha demostrado ser ineficaz, es la esterilización del plasma liquido con radiaciones ultravioleta. El otro metodo es el uso de la ß-propiolactona que se ha desechado tambien рог las alteraciones que producía en la estructura de las protefnas plasmáticas. El objeto de este trabajo, que debe considerarse como una nota preliminar, es presentar los resultados obtenidos al esterilizar por medio de radiaciones gamma ({sup 60}Co, 1,3316 MeV) con dosis variables de 2, 2,5 у 3 Mrad un producto que, como el plasma que contiene proteínas muy lábiles, podría ser fácilmente alterado en su estructura por la radiacion. Tambien se sometieron a las mismas dosis: fibrogeno puro, gamma globulina pura у albúmina de origen humano; los resultados fueron muy satisfacforios, ya que quedo de manifiesto que no se pudieron demostrar alteraciones apreciables en cuanto a su estructura, solubilidad у características bioquímicas. Mediante pruebas simples de coagulacion se puso de manifiesto que algunas de las proteínas que intervienen en este mecanismo, como la protrombina, el factor Power- Stuart у el factor Hageman, prácticamente no se alteraban. Todas las muestras de plasma у de las diferentes proteínas fueron previamente liofilizadas, procurando que el contenido máximo de humedad fuera de 0,034%, con el objeto de evitar la ionizacion del contenido de agua que la tiansformaría en agua oxigenada que alteraría у oxidaría las proteínas. Los plasmas liofilizados previamente contaminados con diferentes cepas у virus demostraron wue con dosis tan bajas como 2 Mrad quedaban esteriles. Por último, se puso de manifiesto que este es un metodo sencillo у práctico, ya que la esterilizacion puede verificarse en el envase final. (author)}
place = {IAEA}
year = {1967}
month = {Sep}
}