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Translocation of {sup 3}H-DNA, {sup 131}I-ribonuclease and {sup 3}H-DNA {sup 131}I-ribonuclease complexes in germinated barley grains; Translocation des ADN{sup 3}H, RNase{sup 131}I et complexes ADN{sup 3}H - RNase {sup 131}I dans les orges en germination

Conference:

Abstract

Barley grains, after germinating for 11 hours in the presence of water, were cut into sections at the end opposite the embryo. They were incubated in solutions of {sup 3}H-DNA, {sup 13I}I-ribonuclease, and {sup 3}H-DNA {sup 131}I-ribonuclease complex for three hours. They were then placed in a water-saturated atmosphere for 24 hours. At this stage the different organs of the seedlings were separated and homogenized in a solution containing 0.15M sodium chloride and 0.1 M sodium ethylenediaminetetraacetate at pH 7. By measuring the radioactivity found in the homogenates one can estimate the penetration of the macromolecules under study. The results show that the quantity found varies from one case to the other and depends both on the nature of the macromolecule and of the organ studied. (author) [French] Des orges qui ont germé pendant 11 h en présence d’eau sont sectionnées au bout opposé à l’embryon. Elles sont incubées avec des solutions d’ADN{sup 3}H, de RNase{sup 131}1 et de complexe ADN{sup 3}H - RNase {sup 131}I, pendant 3 h. Elles sont ensuite placées dans une atmosphère saturée d'eau pendant 24 h. A ce moment, les différents organes des plantules sont séparés et homogénéisés en présence d'une solution 0,15M en NaCl  More>>
Authors:
Tshitenge, G.; [1]  Ledoux, L. [2] 
  1. Centre nucléaire TRICO, Kinshasa (Congo, The Democratic Republic of the)
  2. Centre d’étude de l'énergie nucléaire Mol (Belgium)
Publication Date:
Jan 15, 1970
Product Type:
Conference
Resource Relation:
Conference: Symposium on the peaceful uses of atomic energy in Africa, Kinshasa (Congo, The Democratic Republic of the), 28 Jul - 1 Aug 1969; Other Information: Refs., 2 figs., 5 tabs.; Related Information: In: Peaceful uses of atomic energy in Africa. Proceedings of a symposium| 595 p.
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; BARLEY; DNA; EMBRYOS; HOMOGENATES; IODINE 131; PH VALUE; RNA-ASE; SEEDLINGS; SODIUM; SODIUM CHLORIDES; SOLUTIONS; TRITIUM; WATER
OSTI ID:
22423925
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Nuclear Science Commission of the Democratic Republic of the Congo, Kinshasa (Congo, The Democratic Republic of the)
Country of Origin:
IAEA
Language:
French
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA15M0355129852
Submitting Site:
INIS
Size:
page(s) 297-304
Announcement Date:
Jan 11, 2016

Conference:

Citation Formats

Tshitenge, G., and Ledoux, L. Translocation of {sup 3}H-DNA, {sup 131}I-ribonuclease and {sup 3}H-DNA {sup 131}I-ribonuclease complexes in germinated barley grains; Translocation des ADN{sup 3}H, RNase{sup 131}I et complexes ADN{sup 3}H - RNase {sup 131}I dans les orges en germination. IAEA: N. p., 1970. Web.
Tshitenge, G., & Ledoux, L. Translocation of {sup 3}H-DNA, {sup 131}I-ribonuclease and {sup 3}H-DNA {sup 131}I-ribonuclease complexes in germinated barley grains; Translocation des ADN{sup 3}H, RNase{sup 131}I et complexes ADN{sup 3}H - RNase {sup 131}I dans les orges en germination. IAEA.
Tshitenge, G., and Ledoux, L. 1970. "Translocation of {sup 3}H-DNA, {sup 131}I-ribonuclease and {sup 3}H-DNA {sup 131}I-ribonuclease complexes in germinated barley grains; Translocation des ADN{sup 3}H, RNase{sup 131}I et complexes ADN{sup 3}H - RNase {sup 131}I dans les orges en germination." IAEA.
@misc{etde_22423925,
title = {Translocation of {sup 3}H-DNA, {sup 131}I-ribonuclease and {sup 3}H-DNA {sup 131}I-ribonuclease complexes in germinated barley grains; Translocation des ADN{sup 3}H, RNase{sup 131}I et complexes ADN{sup 3}H - RNase {sup 131}I dans les orges en germination}
author = {Tshitenge, G., and Ledoux, L.}
abstractNote = {Barley grains, after germinating for 11 hours in the presence of water, were cut into sections at the end opposite the embryo. They were incubated in solutions of {sup 3}H-DNA, {sup 13I}I-ribonuclease, and {sup 3}H-DNA {sup 131}I-ribonuclease complex for three hours. They were then placed in a water-saturated atmosphere for 24 hours. At this stage the different organs of the seedlings were separated and homogenized in a solution containing 0.15M sodium chloride and 0.1 M sodium ethylenediaminetetraacetate at pH 7. By measuring the radioactivity found in the homogenates one can estimate the penetration of the macromolecules under study. The results show that the quantity found varies from one case to the other and depends both on the nature of the macromolecule and of the organ studied. (author) [French] Des orges qui ont germé pendant 11 h en présence d’eau sont sectionnées au bout opposé à l’embryon. Elles sont incubées avec des solutions d’ADN{sup 3}H, de RNase{sup 131}1 et de complexe ADN{sup 3}H - RNase {sup 131}I, pendant 3 h. Elles sont ensuite placées dans une atmosphère saturée d'eau pendant 24 h. A ce moment, les différents organes des plantules sont séparés et homogénéisés en présence d'une solution 0,15M en NaCl et 0,1M en éthylènediamine-tétracétate de Na à pH 7. La mesure de la radioactivité retrouvée dans les homogénats permet d'évaluer la pénétration des macromolécules considérées. Les résultats montrent que la quantité retrouvée varie d'un cas â l'autre et dépend à la fois de la nature de la macromolécule et de l'organe considéré. (author)}
place = {IAEA}
year = {1970}
month = {Jan}
}