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Microbial communities in bentonite formations and their interactions with uranium

Abstract

Highlights: • Microbial diversity of Spanish bentonites was studied. • High number of aerobe and facultative anaerobe microbes were isolated from bentonites. • Natural bentonite microbes are able to tolerate high U concentrations. • U is immobilized by the cells of the strain Rhodotorula mucilaginosa BII-R8 as U(VI) phosphates. - Abstract: A reliable performance assessment of deep geological disposal of nuclear waste depends on better knowledge of radionuclide interactions with natural microbes of geological formations (granitic rock, clay, salts) used to host these disposal systems. In Spain, clay deposits from Cabo de Gata region, Almeria, are investigated for this purpose. The present work characterizes the culture-dependent microbial diversity of two bentonite samples (BI and BII) recovered from Spanish clay deposits. The evaluation of aerobe and facultative anaerobe microbial populations shows the presence of a high number of cultivable bacteria (e.g. Stenotrophomonas, Micrococcus, Arthrobacter, Kocuria, Sphingomonas, Bacillus, Pseudomonas, etc.) affiliated to three phyla Proteobacteria, Actinobacteria, and Firmicutes. In addition, a pigmented yeast strain BII-R8 related to Rhodotorula mucilaginosa was also recovered from these formations. The minimal inhibitory concentrations of uranium for the growth of these natural isolates were found to range from 4 to 10.0 mM. For instance, strain R. mucilaginosa  More>>
Publication Date:
Oct 15, 2014
Product Type:
Journal Article
Resource Relation:
Journal Name: Applied Geochemistry; Journal Volume: 49; Conference: Goldschmidt conference: Session on hazardous waste in the geosphere: geochemistry for risk assessment, Florence (Italy), 25-30 Aug 2013; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
54 ENVIRONMENTAL SCIENCES; 58 GEOSCIENCES; BACILLUS; BENTONITE; BIOGEOCHEMISTRY; BIOREMEDIATION; CELL WALL; CONCENTRATION RATIO; GEOLOGIC FORMATIONS; MICROCOCCUS; PHOSPHATE MINERALS; PHOSPHATES; PRECIPITATION; PSEUDOMONAS; RADIOISOTOPES; ROCKS; SALTS; SPAIN; STRAINS; TRANSMISSION ELECTRON MICROSCOPY; URANIUM; YEASTS
OSTI ID:
22400515
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0883-2927; CODEN: APPGEY; Other: PII: S0883-2927(14)00149-8; TRN: GB15R4319106272
Availability:
Available from http://dx.doi.org/10.1016/j.apgeochem.2014.06.022
Submitting Site:
INIS
Size:
page(s) 77-86
Announcement Date:
Nov 11, 2015

Citation Formats

López-Fernández, Margarita, Fernández-Sanfrancisco, Omar, Moreno-García, Alberto, Martín-Sánchez, Inés, Sánchez-Castro, Iván, and Merroun, Mohamed Larbi, E-mail: merroun@ugr.es. Microbial communities in bentonite formations and their interactions with uranium. United Kingdom: N. p., 2014. Web. doi:10.1016/J.APGEOCHEM.2014.06.022.
López-Fernández, Margarita, Fernández-Sanfrancisco, Omar, Moreno-García, Alberto, Martín-Sánchez, Inés, Sánchez-Castro, Iván, & Merroun, Mohamed Larbi, E-mail: merroun@ugr.es. Microbial communities in bentonite formations and their interactions with uranium. United Kingdom. doi:10.1016/J.APGEOCHEM.2014.06.022.
López-Fernández, Margarita, Fernández-Sanfrancisco, Omar, Moreno-García, Alberto, Martín-Sánchez, Inés, Sánchez-Castro, Iván, and Merroun, Mohamed Larbi, E-mail: merroun@ugr.es. 2014. "Microbial communities in bentonite formations and their interactions with uranium." United Kingdom. doi:10.1016/J.APGEOCHEM.2014.06.022. https://www.osti.gov/servlets/purl/10.1016/J.APGEOCHEM.2014.06.022.
@misc{etde_22400515,
title = {Microbial communities in bentonite formations and their interactions with uranium}
author = {López-Fernández, Margarita, Fernández-Sanfrancisco, Omar, Moreno-García, Alberto, Martín-Sánchez, Inés, Sánchez-Castro, Iván, and Merroun, Mohamed Larbi, E-mail: merroun@ugr.es}
abstractNote = {Highlights: • Microbial diversity of Spanish bentonites was studied. • High number of aerobe and facultative anaerobe microbes were isolated from bentonites. • Natural bentonite microbes are able to tolerate high U concentrations. • U is immobilized by the cells of the strain Rhodotorula mucilaginosa BII-R8 as U(VI) phosphates. - Abstract: A reliable performance assessment of deep geological disposal of nuclear waste depends on better knowledge of radionuclide interactions with natural microbes of geological formations (granitic rock, clay, salts) used to host these disposal systems. In Spain, clay deposits from Cabo de Gata region, Almeria, are investigated for this purpose. The present work characterizes the culture-dependent microbial diversity of two bentonite samples (BI and BII) recovered from Spanish clay deposits. The evaluation of aerobe and facultative anaerobe microbial populations shows the presence of a high number of cultivable bacteria (e.g. Stenotrophomonas, Micrococcus, Arthrobacter, Kocuria, Sphingomonas, Bacillus, Pseudomonas, etc.) affiliated to three phyla Proteobacteria, Actinobacteria, and Firmicutes. In addition, a pigmented yeast strain BII-R8 related to Rhodotorula mucilaginosa was also recovered from these formations. The minimal inhibitory concentrations of uranium for the growth of these natural isolates were found to range from 4 to 10.0 mM. For instance, strain R. mucilaginosa BII-R8 was shown to tolerate up to 8 mM of U. Flow cytometry studies indicated that the high U tolerance of this yeast isolate is a biologically mediated process. Microscopically dense intracellular and cell wall-bound precipitates were observed by Scanning Transmission Electron Microscopy-High-Angle Annular Dark-Field (STEM-HAADF). Energy Dispersive X-ray (EDX) element-distribution maps showed the presence of U and P within these accumulates, indicating the ability of cells to precipitate U as U(VI) phosphate minerals. Fundamental understanding of the microbial diversity of clays and microbial interaction with radionuclides will be useful in predicting the microbial impacts on the performance of the waste repositories, as well as in the development of bioremediation strategies for U contaminated sites.}
doi = {10.1016/J.APGEOCHEM.2014.06.022}
journal = {Applied Geochemistry}
volume = {49}
journal type = {AC}
place = {United Kingdom}
year = {2014}
month = {Oct}
}