Abstract
A genetic screen for mutants of Arabidopsis that are hypersensitive to UV light was developed and used to isolate a new mutant designated uvh1. UV hypersensitivity in uvh1 was due to a single recessive trait that is probably located on chromosome 3. Although isolated as hypersensitive to an acute exposure to UV-C light, uvh1 was also hypersensitive to UV-B wavelengths, which are present in sunlight that reaches the earth's surface. UV-B damage to both wild-type and uvh1 plants could be significantly reduced by subsequent exposure of UV-irradiated plants to photoreactivating light, showing that photoreactivation of UV-B damage is important for plant viability and that uvh1 plants are not defective in photoreactivation. A new assay for DNA damage, the Dral assay, was developed and used to show that exposure of wild-type and uvh1 plants to a given dose of UV light induces the same amount of damage in chloroplast and nuclear DNA. Thus, uvh1 is not defective in a UV protective mechanism. uvh1 plants were also found to be hypersensitive to ionizing radiation. These results suggest that uvh1 is defective in a repair or tolerance mechanism that normally provides plants with resistance to several types of DNA damage.
Citation Formats
Harlow, G. R., Jenkins, M. E., Pittalwala, T. S., and Mount, D. W.
Isolation of uvh1, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation.
FAO: N. p.,
1994.
Web.
doi:10.2307/3869641.
Harlow, G. R., Jenkins, M. E., Pittalwala, T. S., & Mount, D. W.
Isolation of uvh1, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation.
FAO.
https://doi.org/10.2307/3869641
Harlow, G. R., Jenkins, M. E., Pittalwala, T. S., and Mount, D. W.
1994.
"Isolation of uvh1, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation."
FAO.
https://doi.org/10.2307/3869641.
@misc{etde_22399556,
title = {Isolation of uvh1, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation}
author = {Harlow, G. R., Jenkins, M. E., Pittalwala, T. S., and Mount, D. W.}
abstractNote = {A genetic screen for mutants of Arabidopsis that are hypersensitive to UV light was developed and used to isolate a new mutant designated uvh1. UV hypersensitivity in uvh1 was due to a single recessive trait that is probably located on chromosome 3. Although isolated as hypersensitive to an acute exposure to UV-C light, uvh1 was also hypersensitive to UV-B wavelengths, which are present in sunlight that reaches the earth's surface. UV-B damage to both wild-type and uvh1 plants could be significantly reduced by subsequent exposure of UV-irradiated plants to photoreactivating light, showing that photoreactivation of UV-B damage is important for plant viability and that uvh1 plants are not defective in photoreactivation. A new assay for DNA damage, the Dral assay, was developed and used to show that exposure of wild-type and uvh1 plants to a given dose of UV light induces the same amount of damage in chloroplast and nuclear DNA. Thus, uvh1 is not defective in a UV protective mechanism. uvh1 plants were also found to be hypersensitive to ionizing radiation. These results suggest that uvh1 is defective in a repair or tolerance mechanism that normally provides plants with resistance to several types of DNA damage.}
doi = {10.2307/3869641}
journal = []
issue = {2}
volume = {6}
journal type = {AC}
place = {FAO}
year = {1994}
month = {Feb}
}
title = {Isolation of uvh1, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation}
author = {Harlow, G. R., Jenkins, M. E., Pittalwala, T. S., and Mount, D. W.}
abstractNote = {A genetic screen for mutants of Arabidopsis that are hypersensitive to UV light was developed and used to isolate a new mutant designated uvh1. UV hypersensitivity in uvh1 was due to a single recessive trait that is probably located on chromosome 3. Although isolated as hypersensitive to an acute exposure to UV-C light, uvh1 was also hypersensitive to UV-B wavelengths, which are present in sunlight that reaches the earth's surface. UV-B damage to both wild-type and uvh1 plants could be significantly reduced by subsequent exposure of UV-irradiated plants to photoreactivating light, showing that photoreactivation of UV-B damage is important for plant viability and that uvh1 plants are not defective in photoreactivation. A new assay for DNA damage, the Dral assay, was developed and used to show that exposure of wild-type and uvh1 plants to a given dose of UV light induces the same amount of damage in chloroplast and nuclear DNA. Thus, uvh1 is not defective in a UV protective mechanism. uvh1 plants were also found to be hypersensitive to ionizing radiation. These results suggest that uvh1 is defective in a repair or tolerance mechanism that normally provides plants with resistance to several types of DNA damage.}
doi = {10.2307/3869641}
journal = []
issue = {2}
volume = {6}
journal type = {AC}
place = {FAO}
year = {1994}
month = {Feb}
}