You need JavaScript to view this

Effect of extraction method and orientin content on radio-protective effect of tulsi extracts

Conference:

Abstract

Extract of tulsi leaves (Ocimum sanctum) has been reported for its radioprotective efficacy. In our initial studies we observed significant variation in the survival of irradiated mice with different batches of tulsi extracts and therefore we employed different extraction methods on leaves collected during various seasons from different localities to study any variation in the radioprotective efficacy. Orientin, a component of tulsi extract, was considered a marker. Mice whole body survival (at 10 Gy lethal whole body irradiation) study and day 11 endo-CFU-s assay (at 5 Gy WBI) were performed employing 3 treatment schedules, 50 mg/kg or 25 mg/kg b.w (single injection, 30 min irradiation), and 10 mg/kgb.w (one injection per day for 5 day, last injection being 30 min before irradiation). Single dose of 25 mg/kg b.w (both aqueous and alcoholic) did not provide any significant survival benefit. The orientin concentrations in the extracts tested varied from 3.3 to 9.91 mg/g extract as studied by HPLC method. With a single administration (i.p) of 50 mg/kg, the aqueous extract from leaves of monsoon season had an orientin content of 9.91 mg/g extract and gave a survival of 60% with a CFU-s count of 37, while extract of leaf summer leaves  More>>
Authors:
Tiwari, Mrinalini; Dwarakanath, B. S.; Agrawala, Paban K., E-mail: pkagrawal@gmail.com; [1]  Murugan, R.; Parimelazhagan, T.; [2]  Uma Devi, P.; [3]  Gota, V.; Sarin, R. K. [4] 
  1. Institute of Nuclear Medicine and Allied Sciences, Delhi (India)
  2. Department of Botany, Bharathiar University, Coimbatore (India)
  3. ARA-B-3SA, Plavilakonam,Trivandrum (India)
  4. Advanced Centre for Treatment Research and Education in Cancer, Navi Mumbai (India)
Publication Date:
Jul 01, 2014
Product Type:
Conference
Resource Relation:
Conference: ICRB-2014: international conference on radiation biology: frontiers in radiobiology - immunomodulation, countermeasures and therapeutics, New Delhi (India), 11-13 Nov 2014; Related Information: In: Proceedings of the international conference on radiation biology: frontiers in radiobiology - immunomodulation, countermeasures and therapeutics: abstract book, souvenir and scientific programme| 314 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; MEDICINAL PLANTS; MICE; RADIATION DOSES; RADIOPROTECTIVE SUBSTANCES; WHOLE-BODY IRRADIATION
OSTI ID:
22389906
Research Organizations:
Institute of Nuclear Medicine and Allied Sciences, Defence Research Development Organisation, Delhi (India)
Country of Origin:
India
Language:
English
Other Identifying Numbers:
TRN: IN1502584095567
Submitting Site:
INIS
Size:
page(s) 202
Announcement Date:
Oct 24, 2015

Conference:

Citation Formats

Tiwari, Mrinalini, Dwarakanath, B. S., Agrawala, Paban K., E-mail: pkagrawal@gmail.com, Murugan, R., Parimelazhagan, T., Uma Devi, P., Gota, V., and Sarin, R. K. Effect of extraction method and orientin content on radio-protective effect of tulsi extracts. India: N. p., 2014. Web.
Tiwari, Mrinalini, Dwarakanath, B. S., Agrawala, Paban K., E-mail: pkagrawal@gmail.com, Murugan, R., Parimelazhagan, T., Uma Devi, P., Gota, V., & Sarin, R. K. Effect of extraction method and orientin content on radio-protective effect of tulsi extracts. India.
Tiwari, Mrinalini, Dwarakanath, B. S., Agrawala, Paban K., E-mail: pkagrawal@gmail.com, Murugan, R., Parimelazhagan, T., Uma Devi, P., Gota, V., and Sarin, R. K. 2014. "Effect of extraction method and orientin content on radio-protective effect of tulsi extracts." India.
@misc{etde_22389906,
title = {Effect of extraction method and orientin content on radio-protective effect of tulsi extracts}
author = {Tiwari, Mrinalini, Dwarakanath, B. S., Agrawala, Paban K., E-mail: pkagrawal@gmail.com, Murugan, R., Parimelazhagan, T., Uma Devi, P., Gota, V., and Sarin, R. K.}
abstractNote = {Extract of tulsi leaves (Ocimum sanctum) has been reported for its radioprotective efficacy. In our initial studies we observed significant variation in the survival of irradiated mice with different batches of tulsi extracts and therefore we employed different extraction methods on leaves collected during various seasons from different localities to study any variation in the radioprotective efficacy. Orientin, a component of tulsi extract, was considered a marker. Mice whole body survival (at 10 Gy lethal whole body irradiation) study and day 11 endo-CFU-s assay (at 5 Gy WBI) were performed employing 3 treatment schedules, 50 mg/kg or 25 mg/kg b.w (single injection, 30 min irradiation), and 10 mg/kgb.w (one injection per day for 5 day, last injection being 30 min before irradiation). Single dose of 25 mg/kg b.w (both aqueous and alcoholic) did not provide any significant survival benefit. The orientin concentrations in the extracts tested varied from 3.3 to 9.91 mg/g extract as studied by HPLC method. With a single administration (i.p) of 50 mg/kg, the aqueous extract from leaves of monsoon season had an orientin content of 9.91 mg/g extract and gave a survival of 60% with a CFU-s count of 37, while extract of leaf summer leaves had an orientin content of 4.15 mg/g extract and gave a survival of 50% with a CFU-s count of 11.6. At the same dose (50 mg/kg), the aqueous extract from the winter season had an orientin content of 3.30 mg/g extract and gave 25% survival with a CFU-s count of 19, while the ethanolic extract had an orientin content of 7.70 mg/g extract and gave a survival of 50% with a CFU-s count of 13. These observations suggest that different climatic factors, orientin content and the doses of administration are important factors regulating radioprotection afforded by different extracts of tulsi. (author)}
place = {India}
year = {2014}
month = {Jul}
}