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Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats

Abstract

Cancer-cachexia induces a variety of metabolic disorders on protein turnorver, decreasing protein synthesis and increasing protein degradation. Controversly, insulin, other hormones, and branched-chain amino acids, especially leucine, stimulate protein synthesis and modulate the activity of translation initiation factors involved in protein synthesis. Since the tumour effects are more pronounced when associated with pregnancy, ehancing muscle-wasting proteolysis, in this study, the influence of a leucine-rich diet on the protein synthesis caused by cancer were investigated. Pregnant rats with or without Walker 256 tumour were distributed into six groups. During 20 days of experiment, three groups were fed with a control diet: C – pregnant control, W – tumour-bearing, and P – pair-fed, which received the same amount of food as ingested by the W group; three other groups of pregnant rats were fed a leucine-rich diet: L – pregnant leucine, WL – tumour-bearing, and PL – pair-fed, which received the same amount of food as ingested by the WL group. The gastrocnemius muscle of WL rats showed increased incorporation of leucine in protein compared to W rats; the leucine-rich diet also prevented the decrease in plasma insulin normally seen in W. The expression of translation initiation factors increased when tumour-bearing rats  More>>
Authors:
Ventrucci, Gislaine; [1]  Mello, Maria Alice R; [2]  Gomes-Marcondes, Maria Cristina C [1] 
  1. Laboratório de Nutrição e Câncer, Departamento de Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, 13083-970, São Paulo (Brazil)
  2. Departamento de Fisiologia e Biofísica, Instituto Biociências, Universidade Estadual de São Paulo, UNESP, Rio Claro, 13506-900, São Paulo (Brazil)
Publication Date:
Mar 06, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: BMC cancer (Online); Journal Volume: 7; Other Information: PMCID: PMC1832207; PUBLISHER-ID: 1471-2407-7-42; PMID: 17341295; OAI: oai:pubmedcentral.nih.gov:1832207; Copyright (c) 2007 Ventrucci et al; licensee BioMed Central Ltd.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; AUGMENTATION; DIET; INSULIN; LEUCINE; MUSCLES; NEOPLASMS; PREGNANCY; RATS; SYNTHESIS
OSTI ID:
22380541
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1471-2407; TRN: GB15$2448086055
Availability:
Available from http://dx.doi.org/10.1186/1471-2407-7-42; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1832207
Submitting Site:
INIS
Size:
page(s) 42
Announcement Date:
Aug 21, 2015

Citation Formats

Ventrucci, Gislaine, Mello, Maria Alice R, and Gomes-Marcondes, Maria Cristina C. Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats. United Kingdom: N. p., 2007. Web. doi:10.1186/1471-2407-7-42.
Ventrucci, Gislaine, Mello, Maria Alice R, & Gomes-Marcondes, Maria Cristina C. Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats. United Kingdom. doi:10.1186/1471-2407-7-42.
Ventrucci, Gislaine, Mello, Maria Alice R, and Gomes-Marcondes, Maria Cristina C. 2007. "Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats." United Kingdom. doi:10.1186/1471-2407-7-42. https://www.osti.gov/servlets/purl/10.1186/1471-2407-7-42.
@misc{etde_22380541,
title = {Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats}
author = {Ventrucci, Gislaine, Mello, Maria Alice R, and Gomes-Marcondes, Maria Cristina C}
abstractNote = {Cancer-cachexia induces a variety of metabolic disorders on protein turnorver, decreasing protein synthesis and increasing protein degradation. Controversly, insulin, other hormones, and branched-chain amino acids, especially leucine, stimulate protein synthesis and modulate the activity of translation initiation factors involved in protein synthesis. Since the tumour effects are more pronounced when associated with pregnancy, ehancing muscle-wasting proteolysis, in this study, the influence of a leucine-rich diet on the protein synthesis caused by cancer were investigated. Pregnant rats with or without Walker 256 tumour were distributed into six groups. During 20 days of experiment, three groups were fed with a control diet: C – pregnant control, W – tumour-bearing, and P – pair-fed, which received the same amount of food as ingested by the W group; three other groups of pregnant rats were fed a leucine-rich diet: L – pregnant leucine, WL – tumour-bearing, and PL – pair-fed, which received the same amount of food as ingested by the WL group. The gastrocnemius muscle of WL rats showed increased incorporation of leucine in protein compared to W rats; the leucine-rich diet also prevented the decrease in plasma insulin normally seen in W. The expression of translation initiation factors increased when tumour-bearing rats fed leucine-rich diet, with increase of ~35% for eIF2α and eIF5, ~17% for eIF4E and 20% for eIF4G; the expression of protein kinase S6K1 and protein kinase C was also highly enhanced. The results suggest that a leucine-rich diet increased the protein synthesis in skeletal muscle in tumour-bearing rats possibly through the activation of eIF factors and/or the S6kinase pathway.}
doi = {10.1186/1471-2407-7-42}
journal = {BMC cancer (Online)}
volume = {7}
journal type = {AC}
place = {United Kingdom}
year = {2007}
month = {Mar}
}