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ETDEWEB   /       /    Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism

Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism

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Abstract

The crystal structure of BT2081 from B. thetaiotaomicron reveals a two-domain protein with a putative carbohydrate-binding site in the C-terminal domain. BT2081 from Bacteroides thetaiotaomicron (GenBank accession code NP-810994.1) is a member of a novel protein family consisting of over 160 members, most of which are found in the different classes of Bacteroidetes. Genome-context analysis lends support to the involvement of this family in carbohydrate metabolism, which plays a key role in B. thetaiotaomicron as a predominant bacterial symbiont in the human distal gut microbiome. The crystal structure of BT2081 at 2.05 Å resolution represents the first structure from this new protein family. BT2081 consists of an N-terminal domain, which adopts a β-sandwich immunoglobulin-like fold, and a larger C-terminal domain with a β-sandwich jelly-roll fold. Structural analyses reveal that both domains are similar to those found in various carbohydrate-active enzymes. The C-terminal β-jelly-roll domain contains a potential carbohydrate-binding site that is highly conserved among BT2081 homologs and is situated in the same location as the carbohydrate-binding sites that are found in structurally similar glycoside hydrolases (GHs). However, in BT2081 this site is partially occluded by surrounding loops, which results in a deep solvent-accessible pocket rather than a shallower solvent-exposed cleft.
Authors:
Yeh, Andrew P.; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Abdubek, Polat; [1]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)]; Astakhova, Tamara; [1]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)]; Axelrod, Herbert L.; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Bakolitsa, Constantina; [1]  Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)]; Cai, Xiaohui; [1]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)]; Carlton, Dennis; [1]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)]; Chen, Connie; [1]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)]; Chiu, Hsiu-Ju; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Chiu, Michelle; [1]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)]; Clayton, Thomas; [1]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)]; Das, Debanu; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Deller, Marc C.; [1]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)]; Duan, Lian; Ellrott, Kyle; [1]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)]; Farr, Carol L.; [1]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)]; Feuerhelm, Julie; Grant, Joanna C.; [1]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)]; Grzechnik, Anna; Han, Gye Won; [1]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)]; Jaroszewski, Lukasz; [1]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States); Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)]; Jin, Kevin K.; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Klock, Heath E.; Knuth, Mark W.; [1]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)]; Kozbial, Piotr; [1]  Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)]; Krishna, S. Sri; [1]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States); Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)]; Kumar, Abhinav; Lam, Winnie W.; [1]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)]; Marciano, David; [2]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]; McMullan, Daniel; [2]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)]; Miller, Mitchell D.; [2]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Morse, Andrew T.; [2]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)]; Nigoghossian, Edward; [2]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)]; Nopakun, Amanda; [2]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]; Okach, Linda; Puckett, Christina; [2]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)]; Reyes, Ron; [2]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Tien, Henry J.; [2]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]; Trame, Christine B.; Bedem, Henry van den; [2]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Weekes, Dana; [2]  Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)]; Wooten, Tiffany; [2]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)]; Xu, Qingping; [2]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Hodgson, Keith O.; [2]  Photon Science, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Wooley, John; [2]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)]; Elsliger, Marc-André; [2]  Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]; Deacon, Ashley M.; [2]  Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)]; Godzik, Adam; [2]  Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US); Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)]; Lesley, Scott A.; [2]  Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US); Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]; Wilson, Ian A., E-mail: wilson@scripps.edu [Joint Center for Structural Genomics, http://www.jcsg.org (US); Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]
  1. Joint Center for Structural Genomics, http://www.jcsg.org (United States)
  2. Joint Center for Structural Genomics, http://www.jcsg.org (US)
Publication Date:
Oct 01, 2010
DOI:
https://doi.org/10.1107/S1744309110028228
Product Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 66; Journal Issue: Pt 10; Other Information: PMCID: PMC2954218; PMID: 20944224; PUBLISHER-ID: wd5132; OAI: oai:pubmedcentral.nih.gov:2954218; Copyright (c) Yeh et al. 2010; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTAL STRUCTURE; POTENTIALS; RESOLUTION; SOLVENTS
OSTI ID:
22367350
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1744-3091; CODEN: ACSFCL; Other: PII: S1744309110028228; TRN: GB15$1103072682
Availability:
Available from http://dx.doi.org/10.1107/S1744309110028228; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954218
Submitting Site:
INIS
Size:
page(s) 1287-1296
Announcement Date:
Jul 29, 2015

Journal Article:

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Citation Formats

Yeh, Andrew P., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Abdubek, Polat, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Astakhova, Tamara, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Axelrod, Herbert L., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Bakolitsa, Constantina, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Cai, Xiaohui, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Carlton, Dennis, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Chen, Connie, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Chiu, Hsiu-Ju, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Chiu, Michelle, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Clayton, Thomas, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Das, Debanu, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Deller, Marc C., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Duan, Lian, Ellrott, Kyle, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Farr, Carol L., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Feuerhelm, Julie, Grant, Joanna C., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Grzechnik, Anna, Han, Gye Won, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Jaroszewski, Lukasz, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Jin, Kevin K., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Klock, Heath E., Knuth, Mark W., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Kozbial, Piotr, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Krishna, S. Sri, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Kumar, Abhinav, Lam, Winnie W., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Marciano, David, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], McMullan, Daniel, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Miller, Mitchell D., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Morse, Andrew T., Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Nigoghossian, Edward, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Nopakun, Amanda, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Okach, Linda, Puckett, Christina, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Reyes, Ron, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Tien, Henry J., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Trame, Christine B., Bedem, Henry van den, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Weekes, Dana, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Wooten, Tiffany, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Xu, Qingping, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Hodgson, Keith O., Photon Science, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Wooley, John, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Elsliger, Marc-André, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Deacon, Ashley M., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Godzik, Adam, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Lesley, Scott A., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US), Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Wilson, Ian A., E-mail: wilson@scripps.edu [Joint Center for Structural Genomics, http://www.jcsg.org (US), and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]. Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism. United Kingdom: N. p., 2010. Web. doi:10.1107/S1744309110028228.
Yeh, Andrew P., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Abdubek, Polat, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Astakhova, Tamara, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Axelrod, Herbert L., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Bakolitsa, Constantina, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Cai, Xiaohui, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Carlton, Dennis, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Chen, Connie, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Chiu, Hsiu-Ju, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Chiu, Michelle, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Clayton, Thomas, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Das, Debanu, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Deller, Marc C., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Duan, Lian, Ellrott, Kyle, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Farr, Carol L., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Feuerhelm, Julie, Grant, Joanna C., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Grzechnik, Anna, Han, Gye Won, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Jaroszewski, Lukasz, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Jin, Kevin K., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Klock, Heath E., Knuth, Mark W., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Kozbial, Piotr, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Krishna, S. Sri, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Kumar, Abhinav, Lam, Winnie W., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Marciano, David, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], McMullan, Daniel, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Miller, Mitchell D., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Morse, Andrew T., Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Nigoghossian, Edward, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Nopakun, Amanda, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Okach, Linda, Puckett, Christina, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Reyes, Ron, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Tien, Henry J., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Trame, Christine B., Bedem, Henry van den, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Weekes, Dana, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Wooten, Tiffany, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Xu, Qingping, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Hodgson, Keith O., Photon Science, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Wooley, John, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Elsliger, Marc-André, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Deacon, Ashley M., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Godzik, Adam, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Lesley, Scott A., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US), Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Wilson, Ian A., E-mail: wilson@scripps.edu [Joint Center for Structural Genomics, http://www.jcsg.org (US), & Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]. Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism. United Kingdom. https://doi.org/10.1107/S1744309110028228
Yeh, Andrew P., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Abdubek, Polat, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Astakhova, Tamara, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Axelrod, Herbert L., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Bakolitsa, Constantina, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Cai, Xiaohui, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Carlton, Dennis, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Chen, Connie, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Chiu, Hsiu-Ju, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Chiu, Michelle, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Clayton, Thomas, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Das, Debanu, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Deller, Marc C., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Duan, Lian, Ellrott, Kyle, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Farr, Carol L., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Feuerhelm, Julie, Grant, Joanna C., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Grzechnik, Anna, Han, Gye Won, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Jaroszewski, Lukasz, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Jin, Kevin K., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Klock, Heath E., Knuth, Mark W., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Kozbial, Piotr, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Krishna, S. Sri, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Kumar, Abhinav, Lam, Winnie W., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Marciano, David, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], McMullan, Daniel, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Miller, Mitchell D., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Morse, Andrew T., Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Nigoghossian, Edward, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Nopakun, Amanda, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Okach, Linda, Puckett, Christina, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Reyes, Ron, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Tien, Henry J., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Trame, Christine B., Bedem, Henry van den, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Weekes, Dana, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Wooten, Tiffany, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Xu, Qingping, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Hodgson, Keith O., Photon Science, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Wooley, John, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Elsliger, Marc-André, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Deacon, Ashley M., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Godzik, Adam, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Lesley, Scott A., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US), Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Wilson, Ian A., E-mail: wilson@scripps.edu [Joint Center for Structural Genomics, http://www.jcsg.org (US), and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]. 2010. "Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism." United Kingdom. https://doi.org/10.1107/S1744309110028228.
@misc{etde_22367350,
title = {Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism}
 author = {Yeh, Andrew P., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Abdubek, Polat, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Astakhova, Tamara, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Axelrod, Herbert L., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Bakolitsa, Constantina, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Cai, Xiaohui, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Carlton, Dennis, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Chen, Connie, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Chiu, Hsiu-Ju, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Chiu, Michelle, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Clayton, Thomas, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Das, Debanu, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Deller, Marc C., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Duan, Lian, Ellrott, Kyle, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States)], Farr, Carol L., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Feuerhelm, Julie, Grant, Joanna C., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Grzechnik, Anna, Han, Gye Won, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (United States)], Jaroszewski, Lukasz, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Jin, Kevin K., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Klock, Heath E., Knuth, Mark W., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States)], Kozbial, Piotr, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Krishna, S. Sri, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (United States), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (United States)], Kumar, Abhinav, Lam, Winnie W., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (United States)], Marciano, David, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], McMullan, Daniel, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Miller, Mitchell D., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Morse, Andrew T., Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Nigoghossian, Edward, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Nopakun, Amanda, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Okach, Linda, Puckett, Christina, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Reyes, Ron, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Tien, Henry J., Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Trame, Christine B., Bedem, Henry van den, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Weekes, Dana, Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Wooten, Tiffany, Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US)], Xu, Qingping, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Hodgson, Keith O., Photon Science, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Wooley, John, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US)], Elsliger, Marc-André, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Deacon, Ashley M., Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA (US)], Godzik, Adam, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA (US), Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, CA (US)], Lesley, Scott A., Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, CA (US), Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)], Wilson, Ian A., E-mail: wilson@scripps.edu [Joint Center for Structural Genomics, http://www.jcsg.org (US), and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA (US)]}
 abstractNote = {The crystal structure of BT2081 from B. thetaiotaomicron reveals a two-domain protein with a putative carbohydrate-binding site in the C-terminal domain. BT2081 from Bacteroides thetaiotaomicron (GenBank accession code NP-810994.1) is a member of a novel protein family consisting of over 160 members, most of which are found in the different classes of Bacteroidetes. Genome-context analysis lends support to the involvement of this family in carbohydrate metabolism, which plays a key role in B. thetaiotaomicron as a predominant bacterial symbiont in the human distal gut microbiome. The crystal structure of BT2081 at 2.05 Å resolution represents the first structure from this new protein family. BT2081 consists of an N-terminal domain, which adopts a β-sandwich immunoglobulin-like fold, and a larger C-terminal domain with a β-sandwich jelly-roll fold. Structural analyses reveal that both domains are similar to those found in various carbohydrate-active enzymes. The C-terminal β-jelly-roll domain contains a potential carbohydrate-binding site that is highly conserved among BT2081 homologs and is situated in the same location as the carbohydrate-binding sites that are found in structurally similar glycoside hydrolases (GHs). However, in BT2081 this site is partially occluded by surrounding loops, which results in a deep solvent-accessible pocket rather than a shallower solvent-exposed cleft.}
 doi = {10.1107/S1744309110028228}
 journal = []
 issue = {Pt 10}
 volume = {66}
 journal type = {AC}
 place = {United Kingdom}
 year = {2010}
 month = {Oct}
 }