Abstract
Cisplatin [DDP, cis-dichlorodiammine platinum (II)], a strong cytostatic and antineoplastic agent, was tested on seedlings of cucumber Cucumis sativus L. for its general effect on root development and its particular effects on root cell division and cell growth. DDP was characterized as a radiomimetic compound since both DDP (1·3 × 10{sup -5} M) and γ-irradiation (2·5-10 kGy) drastically and irreversibly stopped development of embryonic lateral root primordia (LRPs) in the radicle by inhibiting both mitotic activity and cell growth. In 20% of the LRPs of DDP-treated roots, cells did not divide at all. Dividing cells completed no more than two cell cycles. These effects were specific because when DDP was available to the roots only at the onset of cell division, cell proliferation and cell growth were similar to that produced by constant incubation. Neither DDP nor γ-irradiation affected non-meristematic cell elongation. It was concluded that cell growth of meristematic cells is closely related to cell division. However, non-meristematic cell growth is independent of DNA damage. This suggests DDP as a tool to reveal these autonomous processes in plants development and to detect tissue compartments in mature plant embryos which contain potentially non-meristematic cells. (author)
Dubrovsky, J. G.
[1]
- Division of Experimental Biology, Center for Biological Research (CIB), PO Box 128, La Paz, BCS 23000 (Mexico)
Citation Formats
Dubrovsky, J. G.
Radiomimetic effect of cisplatin on cucumber root development: the relationship between cell division and cell growth.
FAO: N. p.,
1993.
Web.
doi:10.1006/anbo.1993.1092.
Dubrovsky, J. G.
Radiomimetic effect of cisplatin on cucumber root development: the relationship between cell division and cell growth.
FAO.
https://doi.org/10.1006/anbo.1993.1092
Dubrovsky, J. G.
1993.
"Radiomimetic effect of cisplatin on cucumber root development: the relationship between cell division and cell growth."
FAO.
https://doi.org/10.1006/anbo.1993.1092.
@misc{etde_22366010,
title = {Radiomimetic effect of cisplatin on cucumber root development: the relationship between cell division and cell growth}
author = {Dubrovsky, J. G.}
abstractNote = {Cisplatin [DDP, cis-dichlorodiammine platinum (II)], a strong cytostatic and antineoplastic agent, was tested on seedlings of cucumber Cucumis sativus L. for its general effect on root development and its particular effects on root cell division and cell growth. DDP was characterized as a radiomimetic compound since both DDP (1·3 × 10{sup -5} M) and γ-irradiation (2·5-10 kGy) drastically and irreversibly stopped development of embryonic lateral root primordia (LRPs) in the radicle by inhibiting both mitotic activity and cell growth. In 20% of the LRPs of DDP-treated roots, cells did not divide at all. Dividing cells completed no more than two cell cycles. These effects were specific because when DDP was available to the roots only at the onset of cell division, cell proliferation and cell growth were similar to that produced by constant incubation. Neither DDP nor γ-irradiation affected non-meristematic cell elongation. It was concluded that cell growth of meristematic cells is closely related to cell division. However, non-meristematic cell growth is independent of DNA damage. This suggests DDP as a tool to reveal these autonomous processes in plants development and to detect tissue compartments in mature plant embryos which contain potentially non-meristematic cells. (author)}
doi = {10.1006/anbo.1993.1092}
journal = []
issue = {2}
volume = {72}
journal type = {AC}
place = {FAO}
year = {1993}
month = {Jul}
}
title = {Radiomimetic effect of cisplatin on cucumber root development: the relationship between cell division and cell growth}
author = {Dubrovsky, J. G.}
abstractNote = {Cisplatin [DDP, cis-dichlorodiammine platinum (II)], a strong cytostatic and antineoplastic agent, was tested on seedlings of cucumber Cucumis sativus L. for its general effect on root development and its particular effects on root cell division and cell growth. DDP was characterized as a radiomimetic compound since both DDP (1·3 × 10{sup -5} M) and γ-irradiation (2·5-10 kGy) drastically and irreversibly stopped development of embryonic lateral root primordia (LRPs) in the radicle by inhibiting both mitotic activity and cell growth. In 20% of the LRPs of DDP-treated roots, cells did not divide at all. Dividing cells completed no more than two cell cycles. These effects were specific because when DDP was available to the roots only at the onset of cell division, cell proliferation and cell growth were similar to that produced by constant incubation. Neither DDP nor γ-irradiation affected non-meristematic cell elongation. It was concluded that cell growth of meristematic cells is closely related to cell division. However, non-meristematic cell growth is independent of DNA damage. This suggests DDP as a tool to reveal these autonomous processes in plants development and to detect tissue compartments in mature plant embryos which contain potentially non-meristematic cells. (author)}
doi = {10.1006/anbo.1993.1092}
journal = []
issue = {2}
volume = {72}
journal type = {AC}
place = {FAO}
year = {1993}
month = {Jul}
}