Abstract
An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å. Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation at BL24XU of SPring-8.
Morita, Hiroyuki;
[1]
Kondo, Shin;
Kato, Ryohei;
[2]
Wanibuchi, Kiyofumi;
Noguchi, Hiroshi;
[3]
Sugio, Shigetoshi;
[2]
Abe, Ikuro;
[3]
PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)];
Kohno, Toshiyuki
[1]
- Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511 (Japan)
- Innovation Center Yokohama, Mitsubishi Chemical Corporation, 1000 Kamoshida, Aoba, Yokohama, Kanagawa 227-8502 (Japan)
- School of Pharmaceutical Sciences, University of Shizuoka and the COE21 Program, Shizuoka 422-8526 (Japan)
Citation Formats
Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)], and Kohno, Toshiyuki.
Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata.
United Kingdom: N. p.,
2007.
Web.
doi:10.1107/S1744309107026164.
Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)], & Kohno, Toshiyuki.
Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata.
United Kingdom.
https://doi.org/10.1107/S1744309107026164
Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)], and Kohno, Toshiyuki.
2007.
"Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata."
United Kingdom.
https://doi.org/10.1107/S1744309107026164.
@misc{etde_22360370,
title = {Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata}
author = {Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)], and Kohno, Toshiyuki}
abstractNote = {An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å. Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation at BL24XU of SPring-8.}
doi = {10.1107/S1744309107026164}
journal = []
issue = {Pt 7}
volume = {63}
journal type = {AC}
place = {United Kingdom}
year = {2007}
month = {Jul}
}
title = {Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata}
author = {Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)], and Kohno, Toshiyuki}
abstractNote = {An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å. Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation at BL24XU of SPring-8.}
doi = {10.1107/S1744309107026164}
journal = []
issue = {Pt 7}
volume = {63}
journal type = {AC}
place = {United Kingdom}
year = {2007}
month = {Jul}
}