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Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis

Abstract

P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution. A recombinant form of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis has been crystallized by the hanging-drop vapour-diffusion method using potassium sodium tartrate as a precipitating agent. The crystals belong to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 149.4, c = 159.7 Å. The crystals are most likely to contain one subunit of a dimer in the asymmetric unit, with a V{sub M} value of 3.14 Å{sup 3} Da{sup −1}. Diffraction data were collected to 2.1 Å resolution using synchrotron radiation at the BL5 station of the Photon Factory.
Authors:
Nakajima, Yoshitaka; Ito, Kiyoshi; Xu, Yue; Yamada, Nozomi; Onohara, Yuko; Ito, Takashi; Yoshimoto, Tadashi [1] 
  1. Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521 (Japan)
Publication Date:
Dec 01, 2005
Product Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 61; Journal Issue: Pt 12; Other Information: PMCID: PMC1978158; PMID: 16511231; PUBLISHER-ID: ll5040; OAI: oai:pubmedcentral.nih.gov:1978158; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA)
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; DIFFUSION; DIMERS; PHOTONS; POTASSIUM; RESOLUTION; SODIUM; SPACE GROUPS; SYNCHROTRON RADIATION
OSTI ID:
22356205
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1744-3091; CODEN: ACSFCL; Other: PII: S1744309105035712; TRN: US15$0622061379
Availability:
Available from http://dx.doi.org/10.1107/S1744309105035712; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978158
Submitting Site:
USN
Size:
page(s) 1046-1048
Announcement Date:
Jul 17, 2015

Citation Formats

Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi. Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis. United Kingdom: N. p., 2005. Web. doi:10.1107/S1744309105035712.
Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, & Yoshimoto, Tadashi. Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis. United Kingdom. doi:10.1107/S1744309105035712.
Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi. 2005. "Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis." United Kingdom. doi:10.1107/S1744309105035712. https://www.osti.gov/servlets/purl/10.1107/S1744309105035712.
@misc{etde_22356205,
title = {Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis}
author = {Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi}
abstractNote = {P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution. A recombinant form of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis has been crystallized by the hanging-drop vapour-diffusion method using potassium sodium tartrate as a precipitating agent. The crystals belong to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 149.4, c = 159.7 Å. The crystals are most likely to contain one subunit of a dimer in the asymmetric unit, with a V{sub M} value of 3.14 Å{sup 3} Da{sup −1}. Diffraction data were collected to 2.1 Å resolution using synchrotron radiation at the BL5 station of the Photon Factory.}
doi = {10.1107/S1744309105035712}
journal = {Acta Crystallographica. Section F}
issue = {Pt 12}
volume = {61}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Dec}
}