Abstract
P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution. A recombinant form of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis has been crystallized by the hanging-drop vapour-diffusion method using potassium sodium tartrate as a precipitating agent. The crystals belong to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 149.4, c = 159.7 Å. The crystals are most likely to contain one subunit of a dimer in the asymmetric unit, with a V{sub M} value of 3.14 Å{sup 3} Da{sup −1}. Diffraction data were collected to 2.1 Å resolution using synchrotron radiation at the BL5 station of the Photon Factory.
Nakajima, Yoshitaka;
Ito, Kiyoshi;
Xu, Yue;
Yamada, Nozomi;
Onohara, Yuko;
Ito, Takashi;
Yoshimoto, Tadashi
[1]
- Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521 (Japan)
Citation Formats
Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi.
Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis.
United Kingdom: N. p.,
2005.
Web.
doi:10.1107/S1744309105035712.
Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, & Yoshimoto, Tadashi.
Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis.
United Kingdom.
https://doi.org/10.1107/S1744309105035712
Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi.
2005.
"Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis."
United Kingdom.
https://doi.org/10.1107/S1744309105035712.
@misc{etde_22356205,
title = {Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis}
author = {Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi}
abstractNote = {P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution. A recombinant form of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis has been crystallized by the hanging-drop vapour-diffusion method using potassium sodium tartrate as a precipitating agent. The crystals belong to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 149.4, c = 159.7 Å. The crystals are most likely to contain one subunit of a dimer in the asymmetric unit, with a V{sub M} value of 3.14 Å{sup 3} Da{sup −1}. Diffraction data were collected to 2.1 Å resolution using synchrotron radiation at the BL5 station of the Photon Factory.}
doi = {10.1107/S1744309105035712}
journal = []
issue = {Pt 12}
volume = {61}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Dec}
}
title = {Crystallization and preliminary X-ray characterization of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis}
author = {Nakajima, Yoshitaka, Ito, Kiyoshi, Xu, Yue, Yamada, Nozomi, Onohara, Yuko, Ito, Takashi, and Yoshimoto, Tadashi}
abstractNote = {P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution. A recombinant form of prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis has been crystallized by the hanging-drop vapour-diffusion method using potassium sodium tartrate as a precipitating agent. The crystals belong to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 149.4, c = 159.7 Å. The crystals are most likely to contain one subunit of a dimer in the asymmetric unit, with a V{sub M} value of 3.14 Å{sup 3} Da{sup −1}. Diffraction data were collected to 2.1 Å resolution using synchrotron radiation at the BL5 station of the Photon Factory.}
doi = {10.1107/S1744309105035712}
journal = []
issue = {Pt 12}
volume = {61}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Dec}
}