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Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube

Abstract

Superhydrophilic vertically aligned carbon nanotubes (VACNT-O{sub 2}) were used for the first time as scaffolds for photodynamic therapy (PDT) to induce inhibition of cell division in eukaryotic cells. VACNT-O{sub 2} scaffolds were produced on Ti substrates using plasma enhanced chemical vapor deposition technique and functionalized by oxygen plasma. Scanning electron microscopy (SEM) analysis was performed to characterize the surface changes of the protozoan and interaction with VACNT-O{sub 2}. Characterization of lipid and total protein expression was performed with protozoa that were or not treated with PDT. Quantification of protein was conducted using Qubit fluorometer and separated on a polyacrylamide gel. SEM analysis showed the release of lipid vesicles by protozoa after the PDT. These vesicles were characterized by the PKH26 fluorescent probe. The results demonstrated a greater amount of protein released after PDT than in the control. When analyzing the protein material in polyacrylamide gel, a significant protein expression of approximately 65 kDa was found. A model identified the programmed death of Tritrichomonas foetus after the PDT was also proposed. - Highlights: • VAMWCNT-O{sub 2} used for the first time as scaffolds for study in parasitic protozoan. • VAMWCNT-O{sub 2} films applied to understand spreading mechanisms of parasitic protozoan. •  More>>
Publication Date:
Mar 01, 2014
Product Type:
Journal Article
Resource Relation:
Journal Name: Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems; Journal Volume: 36; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY; ALUMINIUM; CARBON NANOTUBES; CATTLE; CELL DIVISION; CHEMICAL VAPOR DEPOSITION; CONTROL; FILMS; INTERACTIONS; LIPIDS; OXYGEN; PHTHALOCYANINES; PLASMA; PROTOZOA; SCANNING ELECTRON MICROSCOPY; SUBSTRATES
OSTI ID:
22344650
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0928-4931; Other: PII: S0928-4931(13)00667-X; TRN: NL14R9882050984
Availability:
Available from http://dx.doi.org/10.1016/j.msec.2013.12.004
Submitting Site:
NLN
Size:
page(s) 180-186
Announcement Date:
Jun 19, 2015

Citation Formats

Machado, Susane Moreira, Pacheco-Soares, Cristina, Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)], Marciano, Fernanda Roberta, Lobo, Anderson Oliveira, Soares da Silva, Newton, E-mail: nsoares@univap.br [Laboratory of Tissue and Cell Biology, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil), and Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)]. Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube. Netherlands: N. p., 2014. Web. doi:10.1016/J.MSEC.2013.12.004.
Machado, Susane Moreira, Pacheco-Soares, Cristina, Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)], Marciano, Fernanda Roberta, Lobo, Anderson Oliveira, Soares da Silva, Newton, E-mail: nsoares@univap.br [Laboratory of Tissue and Cell Biology, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil), & Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)]. Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube. Netherlands. doi:10.1016/J.MSEC.2013.12.004.
Machado, Susane Moreira, Pacheco-Soares, Cristina, Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)], Marciano, Fernanda Roberta, Lobo, Anderson Oliveira, Soares da Silva, Newton, E-mail: nsoares@univap.br [Laboratory of Tissue and Cell Biology, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil), and Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)]. 2014. "Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube." Netherlands. doi:10.1016/J.MSEC.2013.12.004. https://www.osti.gov/servlets/purl/10.1016/J.MSEC.2013.12.004.
@misc{etde_22344650,
title = {Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube}
author = {Machado, Susane Moreira, Pacheco-Soares, Cristina, Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)], Marciano, Fernanda Roberta, Lobo, Anderson Oliveira, Soares da Silva, Newton, E-mail: nsoares@univap.br [Laboratory of Tissue and Cell Biology, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil), and Laboratory of Dynamics of Cellular Compartments, Development and Research Institute (IP and D), Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000 São José dos Campos, SP (Brazil)]}
abstractNote = {Superhydrophilic vertically aligned carbon nanotubes (VACNT-O{sub 2}) were used for the first time as scaffolds for photodynamic therapy (PDT) to induce inhibition of cell division in eukaryotic cells. VACNT-O{sub 2} scaffolds were produced on Ti substrates using plasma enhanced chemical vapor deposition technique and functionalized by oxygen plasma. Scanning electron microscopy (SEM) analysis was performed to characterize the surface changes of the protozoan and interaction with VACNT-O{sub 2}. Characterization of lipid and total protein expression was performed with protozoa that were or not treated with PDT. Quantification of protein was conducted using Qubit fluorometer and separated on a polyacrylamide gel. SEM analysis showed the release of lipid vesicles by protozoa after the PDT. These vesicles were characterized by the PKH26 fluorescent probe. The results demonstrated a greater amount of protein released after PDT than in the control. When analyzing the protein material in polyacrylamide gel, a significant protein expression of approximately 65 kDa was found. A model identified the programmed death of Tritrichomonas foetus after the PDT was also proposed. - Highlights: • VAMWCNT-O{sub 2} used for the first time as scaffolds for study in parasitic protozoan. • VAMWCNT-O{sub 2} films applied to understand spreading mechanisms of parasitic protozoan. • A release of a protein of approximately 65kDa of protozoan was also observed.}
doi = {10.1016/J.MSEC.2013.12.004}
journal = {Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems}
volume = {36}
journal type = {AC}
place = {Netherlands}
year = {2014}
month = {Mar}
}