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The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates

Abstract

This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine, Decapinol, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37 degree C for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse  More>>
Authors:
Hunter, Allison; Kalathingal, Sajitha; Shrout, Michael; Plummer, Kevin; Looney, Stephen [1] 
  1. Georgia Regents University, College of Dental Medicine, Augusta (United States)
Publication Date:
Jun 15, 2014
Product Type:
Journal Article
Resource Relation:
Journal Name: Imaging Science in Dentistry; Journal Volume: 44; Journal Issue: 2; Other Information: 23 refs, 1 fig, 1 tab
Subject:
60 APPLIED LIFE SCIENCES; BACTERIA; BLOOD; CONTAMINATION; CONTROL; PHOSPHORS; PLATES
OSTI ID:
22319876
Country of Origin:
Korea, Republic of
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 2233-7822; TRN: KR1500622026078
Submitting Site:
KRN
Size:
page(s) 149-154
Announcement Date:
Mar 31, 2015

Citation Formats

Hunter, Allison, Kalathingal, Sajitha, Shrout, Michael, Plummer, Kevin, and Looney, Stephen. The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates. Korea, Republic of: N. p., 2014. Web. doi:10.5624/isd.2014.44.2.149.
Hunter, Allison, Kalathingal, Sajitha, Shrout, Michael, Plummer, Kevin, & Looney, Stephen. The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates. Korea, Republic of. https://doi.org/10.5624/isd.2014.44.2.149
Hunter, Allison, Kalathingal, Sajitha, Shrout, Michael, Plummer, Kevin, and Looney, Stephen. 2014. "The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates." Korea, Republic of. https://doi.org/10.5624/isd.2014.44.2.149.
@misc{etde_22319876,
title = {The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates}
author = {Hunter, Allison, Kalathingal, Sajitha, Shrout, Michael, Plummer, Kevin, and Looney, Stephen}
abstractNote = {This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine, Decapinol, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37 degree C for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.}
doi = {10.5624/isd.2014.44.2.149}
journal = []
issue = {2}
volume = {44}
journal type = {AC}
place = {Korea, Republic of}
year = {2014}
month = {Jun}
}