Abstract
In-vitro mutagenesis followed by micropropagation via axillary bud proliferation in shoot tips of guava (Psidium guajava L.) cultivar Safeda was carried out. Shoot tips were irradiated with 15 to 90Gy gamma rays radiations using {sup 60}Co gamma cell source and cultured on MS medium containing 3.0% sucrose, BAP and glutamine. Shoots proliferation was observed 7 weeks after culture initiation. Higher shoot proliferation rates were recorded on MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutamine. Rooting was observed on half-strength MS medium supplemented with IAA and IBA. Radio sensitivity test was assessed by determining the percentage of shoot tips survival and shoot proliferation rates. The LD50 (The dose at which 50% of the population killed) was observed on 45 Gy. The doses above 75 Gy were found to be lethal to all explants. Similarly, seeds and bud woods of guava Cv. Safeda were exposed to gamma rays at 0.05 to 0.3 kGy and 20 to 100 Gy respectively using {sup 60}Co gamma source. The LD50 for seeds was determined at 0.19 kGy, and 0.3 kGy was found to be lethal. Seeds irradiated with low doses germinated earlier as compared to those which received higher doses of
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Zamir, R.;
[1]
Ali, N.;
Shah, S. Tariq;
Mohammad, T.;
[2]
Ahmad, J.
[3]
- Nuclear Institute for Food and Agriculture (NIFA), Peshawar (Pakistan)
- N.W.F.P. Agricultural University Peshawar, Peshawar (Pakistan)
- Center of Biotechnology, University of Peshawar, Peshawar (Pakistan)
Citation Formats
Zamir, R., Ali, N., Shah, S. Tariq, Mohammad, T., and Ahmad, J.
Guava (Psidium guajava L) improvement using in vivo and in vitro induced mutagenesis.
IAEA: N. p.,
2009.
Web.
Zamir, R., Ali, N., Shah, S. Tariq, Mohammad, T., & Ahmad, J.
Guava (Psidium guajava L) improvement using in vivo and in vitro induced mutagenesis.
IAEA.
Zamir, R., Ali, N., Shah, S. Tariq, Mohammad, T., and Ahmad, J.
2009.
"Guava (Psidium guajava L) improvement using in vivo and in vitro induced mutagenesis."
IAEA.
@misc{etde_22309258,
title = {Guava (Psidium guajava L) improvement using in vivo and in vitro induced mutagenesis}
author = {Zamir, R., Ali, N., Shah, S. Tariq, Mohammad, T., and Ahmad, J.}
abstractNote = {In-vitro mutagenesis followed by micropropagation via axillary bud proliferation in shoot tips of guava (Psidium guajava L.) cultivar Safeda was carried out. Shoot tips were irradiated with 15 to 90Gy gamma rays radiations using {sup 60}Co gamma cell source and cultured on MS medium containing 3.0% sucrose, BAP and glutamine. Shoots proliferation was observed 7 weeks after culture initiation. Higher shoot proliferation rates were recorded on MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutamine. Rooting was observed on half-strength MS medium supplemented with IAA and IBA. Radio sensitivity test was assessed by determining the percentage of shoot tips survival and shoot proliferation rates. The LD50 (The dose at which 50% of the population killed) was observed on 45 Gy. The doses above 75 Gy were found to be lethal to all explants. Similarly, seeds and bud woods of guava Cv. Safeda were exposed to gamma rays at 0.05 to 0.3 kGy and 20 to 100 Gy respectively using {sup 60}Co gamma source. The LD50 for seeds was determined at 0.19 kGy, and 0.3 kGy was found to be lethal. Seeds irradiated with low doses germinated earlier as compared to those which received higher doses of irradiation. The LD50 for surviving bud woods grafted on the rootstocks in the nursery was determined at 60 Gy. There was constant and continuous reduction in the survival rates of buds as the doses increased. The bud woods of guava Cv. Safeda were more radiosensitive than seeds of the same cultivar. The most favourable doses were established between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40), highest fruit size (59mm) and highest fruit weight of (128.38g). The highest number of seeds (300) and 100 seed-weight (2.29g) were recorded at 0.05KGy while the lowest number of seeds (167) and the lowest 100 seed-weight of 1.56g was observed in the 0.30KGy treatments. (author)}
place = {IAEA}
year = {2009}
month = {May}
}
title = {Guava (Psidium guajava L) improvement using in vivo and in vitro induced mutagenesis}
author = {Zamir, R., Ali, N., Shah, S. Tariq, Mohammad, T., and Ahmad, J.}
abstractNote = {In-vitro mutagenesis followed by micropropagation via axillary bud proliferation in shoot tips of guava (Psidium guajava L.) cultivar Safeda was carried out. Shoot tips were irradiated with 15 to 90Gy gamma rays radiations using {sup 60}Co gamma cell source and cultured on MS medium containing 3.0% sucrose, BAP and glutamine. Shoots proliferation was observed 7 weeks after culture initiation. Higher shoot proliferation rates were recorded on MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutamine. Rooting was observed on half-strength MS medium supplemented with IAA and IBA. Radio sensitivity test was assessed by determining the percentage of shoot tips survival and shoot proliferation rates. The LD50 (The dose at which 50% of the population killed) was observed on 45 Gy. The doses above 75 Gy were found to be lethal to all explants. Similarly, seeds and bud woods of guava Cv. Safeda were exposed to gamma rays at 0.05 to 0.3 kGy and 20 to 100 Gy respectively using {sup 60}Co gamma source. The LD50 for seeds was determined at 0.19 kGy, and 0.3 kGy was found to be lethal. Seeds irradiated with low doses germinated earlier as compared to those which received higher doses of irradiation. The LD50 for surviving bud woods grafted on the rootstocks in the nursery was determined at 60 Gy. There was constant and continuous reduction in the survival rates of buds as the doses increased. The bud woods of guava Cv. Safeda were more radiosensitive than seeds of the same cultivar. The most favourable doses were established between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40), highest fruit size (59mm) and highest fruit weight of (128.38g). The highest number of seeds (300) and 100 seed-weight (2.29g) were recorded at 0.05KGy while the lowest number of seeds (167) and the lowest 100 seed-weight of 1.56g was observed in the 0.30KGy treatments. (author)}
place = {IAEA}
year = {2009}
month = {May}
}