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Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines

Abstract

Plant regeneration through somatic embryogenesis from immature zygotic embryos and embryo cultures from mature fruits were achieved in select avocado accession ‘Semil’ and other seedling trees in the Philippines. Embryogenic cultures were induced from immature zygotic embryos of eight (8) avocado genotypes using either SE1 medium (MS + 30 g/l sucrose + 5 mg/l 2, 4-D + 0.5 mg/l BAP) or SE2 medium (MS + 30 g/l sucrose + 0.1 mg/l picloram). Embryogenic cultures of 2 genotypes namely ‘Semil’ and ‘Mainit’ developed into somatic embryos after repeated subcultures in SE2, SE3 (MS + 30 g/l sucrose + 0.1 mg/l TDZ + 0.5 mg/l GA{sub 3}) and SE4 (MS + 30 g/l sucrose + 2 mg/l BAP + 1 mg/l IBA) media. Plant/shoot regeneration from ‘Semil’ somatic embryos was recorded in 3 trials at 16.3, 23.0 and 20.7%, and was affected by culture age, light treatment and media used. R4 regeneration medium (B5 macro salts + MS minor salts and vitamins + 60 g/l sucrose + 400 g/l glu + 2 mg/l BAP + 4.5 g/l Phytagel was found to be the best. Gamma irradiation (10 to 30 Gy) of embryogenic cultures of ‘Semil’ resulted in reduced proliferation and formation of  More>>
Authors:
Avenido, R. A.; [1]  Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)]; Galvez, H. F.; Dimaculangan, J. G.; Welgas, J. N.; Frankie, R. B.; Damasco, O. P. [2] 
  1. Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños (Philippines)
  2. Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)
Publication Date:
May 15, 2009
Product Type:
Technical Report
Report Number:
IAEA-TECDOC-1615
Resource Relation:
Other Information: 34 refs., 13 tabs., 2 figs.; Related Information: In: Induced mutation in tropical fruit trees| 170 p.
Subject:
60 APPLIED LIFE SCIENCES; AVOCADOS; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; CROPS; EMBRYOS; GAMMA RADIATION; GENOTYPE; GERMINATION; GREENHOUSES; IN VITRO; IRRADIATION; MOTHS; MUTATIONS; PHILIPPINES; PLANT BREEDING; RADIATION INDUCED MUTANTS; REGENERATION; ROOTS; SACCHAROSE; SALTS; SCREENING; SEEDLINGS; TISSUE CULTURES; TREES; VITAMINS
OSTI ID:
22309254
Research Organizations:
International Atomic Energy Agency, Plant Breeding and Genetics Section, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISBN 978-92-0-102709-2; ISSN 1011-4289; TRN: XA14M7142015427
Availability:
Available from INIS in electronic form. Also available on-line: http://www-pub.iaea.org/MTCD/Publications/PDF/TE_1615_web.pdf; Enquiries should be addressed to IAEA, Marketing and Sales Unit, Publishing Section, E-mail: sales.publications@iaea.org; Web site: http://www.iaea.org/books
Submitting Site:
INIS
Size:
page(s) 47-69
Announcement Date:
Mar 02, 2015

Citation Formats

Avenido, R. A., Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)], Galvez, H. F., Dimaculangan, J. G., Welgas, J. N., Frankie, R. B., and Damasco, O. P. Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines. IAEA: N. p., 2009. Web.
Avenido, R. A., Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)], Galvez, H. F., Dimaculangan, J. G., Welgas, J. N., Frankie, R. B., & Damasco, O. P. Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines. IAEA.
Avenido, R. A., Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)], Galvez, H. F., Dimaculangan, J. G., Welgas, J. N., Frankie, R. B., and Damasco, O. P. 2009. "Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines." IAEA.
@misc{etde_22309254,
title = {Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines}
author = {Avenido, R. A., Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)], Galvez, H. F., Dimaculangan, J. G., Welgas, J. N., Frankie, R. B., and Damasco, O. P.}
abstractNote = {Plant regeneration through somatic embryogenesis from immature zygotic embryos and embryo cultures from mature fruits were achieved in select avocado accession ‘Semil’ and other seedling trees in the Philippines. Embryogenic cultures were induced from immature zygotic embryos of eight (8) avocado genotypes using either SE1 medium (MS + 30 g/l sucrose + 5 mg/l 2, 4-D + 0.5 mg/l BAP) or SE2 medium (MS + 30 g/l sucrose + 0.1 mg/l picloram). Embryogenic cultures of 2 genotypes namely ‘Semil’ and ‘Mainit’ developed into somatic embryos after repeated subcultures in SE2, SE3 (MS + 30 g/l sucrose + 0.1 mg/l TDZ + 0.5 mg/l GA{sub 3}) and SE4 (MS + 30 g/l sucrose + 2 mg/l BAP + 1 mg/l IBA) media. Plant/shoot regeneration from ‘Semil’ somatic embryos was recorded in 3 trials at 16.3, 23.0 and 20.7%, and was affected by culture age, light treatment and media used. R4 regeneration medium (B5 macro salts + MS minor salts and vitamins + 60 g/l sucrose + 400 g/l glu + 2 mg/l BAP + 4.5 g/l Phytagel was found to be the best. Gamma irradiation (10 to 30 Gy) of embryogenic cultures of ‘Semil’ resulted in reduced proliferation and formation of cotyledonary stage somatic embryos. However, shoot regeneration from somatic embryos from gamma-irradiated cultures was comparable or even higher (17.8 to 26.9%) as compared to the control (18.3%). Over 200 somatic embryo-derived putative variant/mutant lines from tissue culture and gamma irradiation experiments are being maintained as shoot cultures. Due to slow growth and other related problems, micrografting and in vitro rooting were used to rescue and ensure the greenhouse establishment of putative mutant shoots, and fast-track mutant confirmation by genetic analysis. Preliminary genetic analyses by SSR revealed that (a) the 3 asexually propagated ‘Semil’ mother trees are genetically similar, and (b) mutations marked by the generation of a new allele (band) at the SSR locus was evident among the somatic embryo-derived regenerants from non-irradiated embryogenic cultures. Mass screening for mutations will be done on all successfully potted out regenerants. A second protocol involving embryo culture and gamma irradiation was also done. Mature embryos of 9 genotypes were cultured using liquid MS basal medium with 30 g/l sucrose and 2 mg/l BAP, with 100% germination. Germinating zygotic embryos of ‘San Felix’ were also gamma irradiated and the LD{sub 50} between 20 to 30 Gy was established. M{sub 1}V{sub 4} shoots were generated from gamma-irradiated seedlings after subsequent micropropagation cycles. This is the first successful application of tissue culture and gamma irradiation technologies towards the improvement of a woody perennial fruit crop in the Philippines. (author)}
place = {IAEA}
year = {2009}
month = {May}
}