Abstract
Embryogenic mango cultures of three cultivars on semi solid medium were irradiated at 100 Gy: monoembryonic ‘Tommy Atkins’ and ‘Keitt’ and polyembryonic ‘Hindi be Sennara’. Two weeks after irradiation, cultures were inoculated into liquid maintenance medium containing 10% (v/v) culture filtrate of Colletotrichum gloeosporioides Penz. Following two weeks of exposure to culture filtrate, the embryogenic cultures were sub-cultured onto semi solid maintenance medium. Living pro-embryonic masses were manually separated from necrotic tissue four weeks later and were transferred onto semi solid maintenance medium. Somatic embryos were recovered and their shoots have been rescued by ex vitro grafting. Field plantings were established in early 2005; however, the results are inconclusive at the time of writing. (author)
Litz, R. E.
[1]
- Tropical Research and Education Center, University of Florida, Homestead, FL (United States)
Citation Formats
Litz, R. E.
Recovery of mango plants with antrachnose resistance following mutation induction and selection in vitro with the culture filtrate of Colletotrichum gloesporoides Penz.
IAEA: N. p.,
2009.
Web.
Litz, R. E.
Recovery of mango plants with antrachnose resistance following mutation induction and selection in vitro with the culture filtrate of Colletotrichum gloesporoides Penz.
IAEA.
Litz, R. E.
2009.
"Recovery of mango plants with antrachnose resistance following mutation induction and selection in vitro with the culture filtrate of Colletotrichum gloesporoides Penz."
IAEA.
@misc{etde_22309250,
title = {Recovery of mango plants with antrachnose resistance following mutation induction and selection in vitro with the culture filtrate of Colletotrichum gloesporoides Penz}
author = {Litz, R. E.}
abstractNote = {Embryogenic mango cultures of three cultivars on semi solid medium were irradiated at 100 Gy: monoembryonic ‘Tommy Atkins’ and ‘Keitt’ and polyembryonic ‘Hindi be Sennara’. Two weeks after irradiation, cultures were inoculated into liquid maintenance medium containing 10% (v/v) culture filtrate of Colletotrichum gloeosporioides Penz. Following two weeks of exposure to culture filtrate, the embryogenic cultures were sub-cultured onto semi solid maintenance medium. Living pro-embryonic masses were manually separated from necrotic tissue four weeks later and were transferred onto semi solid maintenance medium. Somatic embryos were recovered and their shoots have been rescued by ex vitro grafting. Field plantings were established in early 2005; however, the results are inconclusive at the time of writing. (author)}
place = {IAEA}
year = {2009}
month = {May}
}
title = {Recovery of mango plants with antrachnose resistance following mutation induction and selection in vitro with the culture filtrate of Colletotrichum gloesporoides Penz}
author = {Litz, R. E.}
abstractNote = {Embryogenic mango cultures of three cultivars on semi solid medium were irradiated at 100 Gy: monoembryonic ‘Tommy Atkins’ and ‘Keitt’ and polyembryonic ‘Hindi be Sennara’. Two weeks after irradiation, cultures were inoculated into liquid maintenance medium containing 10% (v/v) culture filtrate of Colletotrichum gloeosporioides Penz. Following two weeks of exposure to culture filtrate, the embryogenic cultures were sub-cultured onto semi solid maintenance medium. Living pro-embryonic masses were manually separated from necrotic tissue four weeks later and were transferred onto semi solid maintenance medium. Somatic embryos were recovered and their shoots have been rescued by ex vitro grafting. Field plantings were established in early 2005; however, the results are inconclusive at the time of writing. (author)}
place = {IAEA}
year = {2009}
month = {May}
}