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Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis

Abstract

The study was conducted to determine the effect of 405-nm diode laser irradiation on periodontopathic bacteria such as Porphyromonas gingivalis in vitro. A diluted suspension of P. gingivalis was irradiated directly with a 405-nm diode laser under conditions of 100 mW-10 sec, 100 mW-20 sec, 200 mW-5 sec, 200 mW-10 sec, 200 mW-20 sec, 400 mW-5 sec, 400 mW-10 sec, and 400 mW-20 sec. The energy density ranged from 2.0 to 16.0 J/cm{sup 2}. The irradiated bacterial suspension was spread on a blood agar plate and growth of the colonies was examined after an anaerobic culture for 7 days. Bacterial growth was inhibited under all irradiation conditions, but the bactericidal effect of the 405-nm diode laser depended on the energy density. More than 97% of bacterial growth was inhibited with irradiation at an energy density > 4.0 J/cm{sup 2}. The mechanism of the bactericidal effect is photochemical, rather than photothermal. These findings suggest that a 405-nm diode laser has a high bactericidal effect on P. gingivalis.
Authors:
Kotoku, Y; Kato, J; Akashi, G; Hirai, Y; [1]  Ishihara, K [2] 
  1. Department of Operative Dentistry, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502 (Japan)
  2. Department of Microbiology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502 (Japan)
Publication Date:
May 15, 2009
Product Type:
Journal Article
Resource Relation:
Journal Name: Laser physics letters (Internet); Journal Volume: 6; Journal Issue: 5; Other Information: Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; AGAR; BACTERIA; BIOLOGICAL RADIATION EFFECTS; BLOOD; ENERGY DENSITY; IN VITRO; IRRADIATION; LASER RADIATION; OPTICS; PHOTOCHEMISTRY; SOLID STATE LASERS; SUSPENSIONS
OSTI ID:
22303038
Country of Origin:
Russian Federation
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1612-202X; TRN: RU13I8363009210
Availability:
Available from http://dx.doi.org/10.1002/lapl.200910011
Submitting Site:
INIS
Size:
page(s) 388-392
Announcement Date:
Jan 30, 2015

Citation Formats

Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K. Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis. Russian Federation: N. p., 2009. Web. doi:10.1002/LAPL.200910011.
Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, & Ishihara, K. Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis. Russian Federation. doi:10.1002/LAPL.200910011.
Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K. 2009. "Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis." Russian Federation. doi:10.1002/LAPL.200910011. https://www.osti.gov/servlets/purl/10.1002/LAPL.200910011.
@misc{etde_22303038,
title = {Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis}
author = {Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K}
abstractNote = {The study was conducted to determine the effect of 405-nm diode laser irradiation on periodontopathic bacteria such as Porphyromonas gingivalis in vitro. A diluted suspension of P. gingivalis was irradiated directly with a 405-nm diode laser under conditions of 100 mW-10 sec, 100 mW-20 sec, 200 mW-5 sec, 200 mW-10 sec, 200 mW-20 sec, 400 mW-5 sec, 400 mW-10 sec, and 400 mW-20 sec. The energy density ranged from 2.0 to 16.0 J/cm{sup 2}. The irradiated bacterial suspension was spread on a blood agar plate and growth of the colonies was examined after an anaerobic culture for 7 days. Bacterial growth was inhibited under all irradiation conditions, but the bactericidal effect of the 405-nm diode laser depended on the energy density. More than 97% of bacterial growth was inhibited with irradiation at an energy density > 4.0 J/cm{sup 2}. The mechanism of the bactericidal effect is photochemical, rather than photothermal. These findings suggest that a 405-nm diode laser has a high bactericidal effect on P. gingivalis.}
doi = {10.1002/LAPL.200910011}
journal = {Laser physics letters (Internet)}
issue = {5}
volume = {6}
journal type = {AC}
place = {Russian Federation}
year = {2009}
month = {May}
}