Abstract
The study was conducted to determine the effect of 405-nm diode laser irradiation on periodontopathic bacteria such as Porphyromonas gingivalis in vitro. A diluted suspension of P. gingivalis was irradiated directly with a 405-nm diode laser under conditions of 100 mW-10 sec, 100 mW-20 sec, 200 mW-5 sec, 200 mW-10 sec, 200 mW-20 sec, 400 mW-5 sec, 400 mW-10 sec, and 400 mW-20 sec. The energy density ranged from 2.0 to 16.0 J/cm{sup 2}. The irradiated bacterial suspension was spread on a blood agar plate and growth of the colonies was examined after an anaerobic culture for 7 days. Bacterial growth was inhibited under all irradiation conditions, but the bactericidal effect of the 405-nm diode laser depended on the energy density. More than 97% of bacterial growth was inhibited with irradiation at an energy density > 4.0 J/cm{sup 2}. The mechanism of the bactericidal effect is photochemical, rather than photothermal. These findings suggest that a 405-nm diode laser has a high bactericidal effect on P. gingivalis.
Kotoku, Y;
Kato, J;
Akashi, G;
Hirai, Y;
[1]
Ishihara, K
[2]
- Department of Operative Dentistry, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502 (Japan)
- Department of Microbiology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502 (Japan)
Citation Formats
Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K.
Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis.
Russian Federation: N. p.,
2009.
Web.
doi:10.1002/LAPL.200910011.
Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, & Ishihara, K.
Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis.
Russian Federation.
https://doi.org/10.1002/LAPL.200910011
Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K.
2009.
"Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis."
Russian Federation.
https://doi.org/10.1002/LAPL.200910011.
@misc{etde_22303038,
title = {Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis}
author = {Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K}
abstractNote = {The study was conducted to determine the effect of 405-nm diode laser irradiation on periodontopathic bacteria such as Porphyromonas gingivalis in vitro. A diluted suspension of P. gingivalis was irradiated directly with a 405-nm diode laser under conditions of 100 mW-10 sec, 100 mW-20 sec, 200 mW-5 sec, 200 mW-10 sec, 200 mW-20 sec, 400 mW-5 sec, 400 mW-10 sec, and 400 mW-20 sec. The energy density ranged from 2.0 to 16.0 J/cm{sup 2}. The irradiated bacterial suspension was spread on a blood agar plate and growth of the colonies was examined after an anaerobic culture for 7 days. Bacterial growth was inhibited under all irradiation conditions, but the bactericidal effect of the 405-nm diode laser depended on the energy density. More than 97% of bacterial growth was inhibited with irradiation at an energy density > 4.0 J/cm{sup 2}. The mechanism of the bactericidal effect is photochemical, rather than photothermal. These findings suggest that a 405-nm diode laser has a high bactericidal effect on P. gingivalis.}
doi = {10.1002/LAPL.200910011}
journal = []
issue = {5}
volume = {6}
journal type = {AC}
place = {Russian Federation}
year = {2009}
month = {May}
}
title = {Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis}
author = {Kotoku, Y, Kato, J, Akashi, G, Hirai, Y, and Ishihara, K}
abstractNote = {The study was conducted to determine the effect of 405-nm diode laser irradiation on periodontopathic bacteria such as Porphyromonas gingivalis in vitro. A diluted suspension of P. gingivalis was irradiated directly with a 405-nm diode laser under conditions of 100 mW-10 sec, 100 mW-20 sec, 200 mW-5 sec, 200 mW-10 sec, 200 mW-20 sec, 400 mW-5 sec, 400 mW-10 sec, and 400 mW-20 sec. The energy density ranged from 2.0 to 16.0 J/cm{sup 2}. The irradiated bacterial suspension was spread on a blood agar plate and growth of the colonies was examined after an anaerobic culture for 7 days. Bacterial growth was inhibited under all irradiation conditions, but the bactericidal effect of the 405-nm diode laser depended on the energy density. More than 97% of bacterial growth was inhibited with irradiation at an energy density > 4.0 J/cm{sup 2}. The mechanism of the bactericidal effect is photochemical, rather than photothermal. These findings suggest that a 405-nm diode laser has a high bactericidal effect on P. gingivalis.}
doi = {10.1002/LAPL.200910011}
journal = []
issue = {5}
volume = {6}
journal type = {AC}
place = {Russian Federation}
year = {2009}
month = {May}
}