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Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area

Abstract

Animal productions (i.e. fish, eggs, milk and dairy products) represent the major source of exposure to dioxins, furans, and dioxin-like (DL) polychlorobiphenyls for humans. The negative effects of these highly toxic and persistent pollutants are mediated by the activation of the aryl hydrocarbon receptor (AHR) that elicits the transcriptional induction of several genes, including those involved in xenobiotic metabolism. Previously we demonstrated the presence and functioning of the AHR signaling pathway in primary cultures of bovine blood lymphocytes. The aim of the present study was to investigate by real time PCR the expression and the inducibility of selected target genes (i.e. AHR, AHR nuclear translocator (ARNT), AHR repressor, CYP1A1 and CYP1B1) in uncultured cells from dairy cows naturally exposed to DL-compounds. The study was carried out on two groups of animals bred in a highly polluted area and characterized by a different degree of contamination, as assessed by bulk milk TEQ values, and a control group reared in an industry free area. Bovine lymphocytes expressed only AHR, ARNT and CYP1B1 genes to a detectable level; moreover, only CYP1B1 expression appeared to be correlated to TEQ values, being higher in the most contaminated group, and decreasing along with animal decontamination. Finally,  More>>
Authors:
Girolami, Flavia; [1]  Spalenza, Veronica; [2]  Carletti, Monica; [1]  Sacchi, Paola; [2]  Rasero, Roberto; [2]  Nebbia, Carlo [1] 
  1. Department of Animal Pathology, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco (Italy)
  2. Department of Animal Production, Epidemiology and Ecology, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco (Italy)
Publication Date:
Apr 15, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Science of the Total Environment; Journal Volume: 450-451; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; COWS; DIOXIN; DMSO; HYDROCARBONS; LYMPHOCYTES; MILK; POLYCHLORINATED BIPHENYLS; POLYMERASE CHAIN REACTION; RECEPTORS
OSTI ID:
22277149
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0048-9697; CODEN: STENDL; Other: PII: S0048-9697(13)00157-5; TRN: NL14S6371098504
Availability:
Available from http://dx.doi.org/10.1016/j.scitotenv.2013.01.095
Submitting Site:
NLN
Size:
page(s) 7-12
Announcement Date:
Nov 18, 2014

Citation Formats

Girolami, Flavia, Spalenza, Veronica, Carletti, Monica, Sacchi, Paola, Rasero, Roberto, and Nebbia, Carlo. Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area. Netherlands: N. p., 2013. Web. doi:10.1016/J.SCITOTENV.2013.01.095.
Girolami, Flavia, Spalenza, Veronica, Carletti, Monica, Sacchi, Paola, Rasero, Roberto, & Nebbia, Carlo. Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area. Netherlands. doi:10.1016/J.SCITOTENV.2013.01.095.
Girolami, Flavia, Spalenza, Veronica, Carletti, Monica, Sacchi, Paola, Rasero, Roberto, and Nebbia, Carlo. 2013. "Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area." Netherlands. doi:10.1016/J.SCITOTENV.2013.01.095. https://www.osti.gov/servlets/purl/10.1016/J.SCITOTENV.2013.01.095.
@misc{etde_22277149,
title = {Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area}
author = {Girolami, Flavia, Spalenza, Veronica, Carletti, Monica, Sacchi, Paola, Rasero, Roberto, and Nebbia, Carlo}
abstractNote = {Animal productions (i.e. fish, eggs, milk and dairy products) represent the major source of exposure to dioxins, furans, and dioxin-like (DL) polychlorobiphenyls for humans. The negative effects of these highly toxic and persistent pollutants are mediated by the activation of the aryl hydrocarbon receptor (AHR) that elicits the transcriptional induction of several genes, including those involved in xenobiotic metabolism. Previously we demonstrated the presence and functioning of the AHR signaling pathway in primary cultures of bovine blood lymphocytes. The aim of the present study was to investigate by real time PCR the expression and the inducibility of selected target genes (i.e. AHR, AHR nuclear translocator (ARNT), AHR repressor, CYP1A1 and CYP1B1) in uncultured cells from dairy cows naturally exposed to DL-compounds. The study was carried out on two groups of animals bred in a highly polluted area and characterized by a different degree of contamination, as assessed by bulk milk TEQ values, and a control group reared in an industry free area. Bovine lymphocytes expressed only AHR, ARNT and CYP1B1 genes to a detectable level; moreover, only CYP1B1 expression appeared to be correlated to TEQ values, being higher in the most contaminated group, and decreasing along with animal decontamination. Finally, lymphocytes from exposed cows displayed a lower inducibility of both CYP1A1 and CYP1B1 after the in vitro treatment with a specific AHR ligand. In conclusion, our results indicate that DL-compound contaminated cows may display significant changes in AHR-target gene expression of circulating lymphocytes. - Highlights: ► The expression of AHR-target genes in blood bovine lymphocytes was evaluated. ► The lymphocyte CYP1B1 expression appears to be related to bulk milk TEQ values. ► Blood lymphocytes from dairy cows might represent a matrix for dioxin biomonitoring.}
doi = {10.1016/J.SCITOTENV.2013.01.095}
journal = {Science of the Total Environment}
volume = {450-451}
journal type = {AC}
place = {Netherlands}
year = {2013}
month = {Apr}
}