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Detection of individual radiosensitivity by radiation–induced micronuclei in human peripheral blood lymphocytes and polymorphisms in DNA repair genes

Abstract

Aim: To investigate the association of two polymorphisms – in XRCC1 gene (Arg399Gln) and in APE1 gene (Asp148Glu) and the radiation induced frequency of micronuclei in human peripheral blood lymphocytes. Material and methods: Genomic DNA from 34 cancer patients and 52 controls were genotyped using PCR–RFLP technique. Micronucleus test (MNT) was performed on 15 cancer patients and 15 controls, before and after in vitro irradiation with 2Gy gamma rays. Results: The data showed that cancer patients had a significantly higher spontaneous frequency of cells with micronuclei than controls (P=0.009). No statistical difference was registered when comparing the mean frequency of cells with micronuclei after in vitro irradiation between these groups. Four subjects were selected as radiosensitive after applying cut–off of the mean frequency of radiation induced micronuclei. Three of them are carriers of the XRCC1 399Gln allele and two of them are carriers of the APE1 148Glu allele. (author)
Authors:
Staynova, A.; Hadjidekova, V.; Popova, L.; Hristova, R.; [1]  Savov, A. [2] 
  1. Radiation Genetics Laboratory, National Centre of Radiobology and Radiation Protection, Sofia (Bulgaria)
  2. National Genetic Laboratory, University Hospital of Obstetrics and Gynecology, Sofia (Bulgaria)
Publication Date:
Jul 01, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Radiation Protection Journal; Journal Volume: 1; Other Information: 2 figs., 3 tabs., 16 refs.
Subject:
61 RADIATION PROTECTION AND DOSIMETRY; EMERGENCY PLANS; GAMMA RADIATION; IN VITRO; KOZLODUY-5 REACTOR; KOZLODUY-6 REACTOR; LYMPHOCYTES; NEOPLASMS; NUCLEOTIDES; PATIENTS
OSTI ID:
22257318
Country of Origin:
Bulgaria
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1314-9199; TRN: BG1400462078516
Availability:
Also available at: http://www.barrp.org/files/spisanie-barrp.pdf
Submitting Site:
INIS
Size:
page(s) 27-31
Announcement Date:
Aug 16, 2014

Citation Formats

Staynova, A., Hadjidekova, V., Popova, L., Hristova, R., and Savov, A. Detection of individual radiosensitivity by radiation–induced micronuclei in human peripheral blood lymphocytes and polymorphisms in DNA repair genes. Bulgaria: N. p., 2013. Web.
Staynova, A., Hadjidekova, V., Popova, L., Hristova, R., & Savov, A. Detection of individual radiosensitivity by radiation–induced micronuclei in human peripheral blood lymphocytes and polymorphisms in DNA repair genes. Bulgaria.
Staynova, A., Hadjidekova, V., Popova, L., Hristova, R., and Savov, A. 2013. "Detection of individual radiosensitivity by radiation–induced micronuclei in human peripheral blood lymphocytes and polymorphisms in DNA repair genes." Bulgaria.
@misc{etde_22257318,
title = {Detection of individual radiosensitivity by radiation–induced micronuclei in human peripheral blood lymphocytes and polymorphisms in DNA repair genes}
author = {Staynova, A., Hadjidekova, V., Popova, L., Hristova, R., and Savov, A.}
abstractNote = {Aim: To investigate the association of two polymorphisms – in XRCC1 gene (Arg399Gln) and in APE1 gene (Asp148Glu) and the radiation induced frequency of micronuclei in human peripheral blood lymphocytes. Material and methods: Genomic DNA from 34 cancer patients and 52 controls were genotyped using PCR–RFLP technique. Micronucleus test (MNT) was performed on 15 cancer patients and 15 controls, before and after in vitro irradiation with 2Gy gamma rays. Results: The data showed that cancer patients had a significantly higher spontaneous frequency of cells with micronuclei than controls (P=0.009). No statistical difference was registered when comparing the mean frequency of cells with micronuclei after in vitro irradiation between these groups. Four subjects were selected as radiosensitive after applying cut–off of the mean frequency of radiation induced micronuclei. Three of them are carriers of the XRCC1 399Gln allele and two of them are carriers of the APE1 148Glu allele. (author)}
journal = {Radiation Protection Journal}
volume = {1}
journal type = {AC}
place = {Bulgaria}
year = {2013}
month = {Jul}
}